FK1706促进周围神经再生作用的实验研究
发布时间：2018-09-05 15:47
【摘要】：目前已知FK506是一种免疫抑制剂，具有显著促进神经再生作用，但其免疫抑制作用限制了其在周围神经损伤修复中的应用。按照去除其免疫抑制结构设计的衍生物FK1706的是否有促进神经再生作用，尚未明了。本研究通过建立大鼠坐骨神经损伤神经移植与神经旁路移植模型，术后应用FK1706，探讨其对修复神经的再生作用。实验分为以下2个部分。 第一部分神经移植术后FK1706促进神经再生作用的实验研究 目的： 探讨神经移植后FK1706促进神经再生作用。 方法： SD大鼠20只，雄性，250-300g，随机分为实验组（FK1706组）和对照组。两组麻醉后，取左侧桡神经10mm备用，显露左侧坐骨神经干，于梨状肌下0.5cm处，用刀片整齐切除8mm长的坐骨神经，将桡神经移植于坐骨神经上，两断端分别行端端吻合。实验组术后当天开始左下肢局部肌肉注射FK1706（0.32mg/kg），持续8周。对照组神经移植术后不做干预，常规喂养。8周后行大鼠左坐骨神经神经电生理检测、左坐骨神经再生有髓神经纤维数及截面积测定、左腓肠肌肌湿重及肌纤维截面积测定。 结果： 1.实验组复合运动动作电位（CMAP）的波幅7.34±1.22mV，对照组6.07±1.18mV，相比差异有显著性（P0.05）。实验组运动神经传导速度（MNCV）6.07±0.95m/s，对照组3.78±0.46m/s，相比差异有显著性（P0.01）。 2.实验组有髓神经纤维数近端1199.53±113.73根，远端701.17±78.40根，远端纤维截面积22.53±6.52μm2，对照组有髓神经纤维数近端1164.73±71.39根，远端532.78±100.68根，远端纤维截面积14.39±3.13μm2，近端有髓神经纤维数相比差异无显著性（P＞0.05），远心端有髓神经纤维数、远端纤维截面积相比差异有显著性（P0.01）。 3.实验组再生有髓神经纤维的髓鞘厚度（mt）0.1523±0.0520μm，再生纤维的直径比（d/D）0.6364±0.1199，对照组再生有髓神经纤维的髓鞘厚度（mt）0.1017±0.0773，再生纤维的直径比（d/D）0.4969±0.1396，相比差异有显著性（P0.05）。 4.实验组腓肠肌肌湿重805.91±98.82mg，对照组腓肠肌肌湿重702.26±78.46mg，相比差异有显著性（P0.05）。实验组肌纤维截面积323.81±34.51μm2，对照组肌纤维截面积213.68±45.77μm2，相比差异有显著性（P0.01）。结论：FK1706能显著改善大鼠神经移植术后运动神经传导速度，增加吻合口远端再生有髓神经纤维数及截面积，增加患肢腓肠肌肌湿重及肌纤维截面积。FK1706具有显著促进大鼠神经移植术后神经再生作用。 第二部分神经旁路移植术后FK1706促进神经再生的实验研究 目的： 探讨神经旁路移植后FK1706促进神经再生作用。方法： SD大鼠20只，雄性，250-300g，随机分为实验组（FK1706组）和对照组。两组麻醉后，取左侧桡神经10mm备用，显露左侧坐骨神经干，用显微镜持针钳钳夹其中点10s，于钳夹点近、远端3mm处神经外膜上，分别开一张直径为1.5mm的窗口，将桡神经与两窗口作端侧吻合。实验组术后当天开始左下肢局部肌肉注射FK1706（0.32mg/kg），持续8周。对照组神经旁路移植术后不做干预，常规喂养。8周后行大鼠左坐骨神经神经电生理检测、左坐骨神经再生有髓神经纤维数及截面积测定、左腓肠肌肌湿重及肌纤维截面积测定。 结果： 1.实验组复合运动动作电位（CMAP）的波幅6.69±0.89mV，对照组5.32±0.80mV，相比差异有显著性（P0.01）。实验组运动神经传导速度（MNCV）5.98±1.14m/s，对照组4.32±0.53m/s，相比差异有显著性（P0.01）。 2.实验组有髓神经纤维数近端1131.67±45.28根，远端708.52±39.85根，远端纤维截面积20.68±6.95μm2，对照组有髓神经纤维数近端1020.70±55.22根，远端468.87±119.67根，远端纤维截面积13.29±2.39μm2，近端有髓神经纤维数相比差异无显著性（P＞0.05），，远心端有髓神经纤维数、远端纤维截面积相比差异有显著性（P0.01）。 3.实验组再生有髓神经纤维的髓鞘厚度（mt）0.1566±0.0659μm，再生纤维的直径比（d/D）0.6269±0.1094，对照组再生有髓神经纤维的髓鞘厚度（mt）0.1022±0.0458，再生纤维的直径比（d/D）0.5101±0.1294，相比差异有显著性（P0.05）。 4.实验组腓肠肌肌湿重753.54±105.91mg，对照组腓肠肌肌湿重598.02±65.02mg，相比差异有显著性（P0.01）。实验组（FK1706组）肌纤维截面积246.55±50.41μm2，对照组肌纤维截面积178.90±33.59μm2，相比差异有显著性（P0.01）。 结论： FK1706能改善大鼠神经旁路移植术后运动神经传导速度，增加吻合口远端再生有髓神经纤维数及截面积、腓肠肌肌湿重及肌纤维截面积。FK1706具有促进大鼠神经旁路移植术后移植神经再生的作用。
[Abstract]:It is known that FK506 is an immunosuppressive agent which can significantly promote nerve regeneration, but its immunosuppressive effect limits its application in the repair of peripheral nerve injury. The regeneration of injured nerve graft and nerve bypass graft was studied with FK1706 after operation. The experiment was divided into the following two parts.
Part one experimental study on the effect of FK1706 on nerve regeneration after nerve transplantation
Objective:
Objective to investigate the effect of FK1706 on nerve regeneration after nerve transplantation.
Method:
20 male SD rats, 250-300 g, were randomly divided into experimental group (FK1706 group) and control group. After anesthesia, the left radial nerve was reserved for 10 mm and the left sciatic nerve trunk was exposed. At 0.5 cm below the piriformis muscle, the sciatic nerve of 8 mm length was resected with a knife blade, and the radial nerve was transplanted to the sciatic nerve. End-to-end anastomosis was performed at both ends of the sciatic nerve. FK1706 (0.32 mg/kg) was injected into the left lower limb for 8 weeks. The control group received no intervention after nerve transplantation and was fed routinely for 8 weeks.
Result:
1. The amplitude of compound motor action potential (CMAP) in the experimental group was 7.34 (+ 1.22 mV) and that in the control group was 6.07 (+ 1.18 mV) with a significant difference (P 0.05). The motor nerve conduction velocity (MNCV) in the experimental group was 6.07 (+ 0.95 m/s) and that in the control group was 3.78 (+ 0.46 m/s), with a significant difference (P 0.01).
2. The number of myelinated nerve fibers in the proximal end was 1199.53+113.73 in the experimental group, 701.17+78.40 in the distal end, 22.53+6.52 micron 2 in the distal end. The number of myelinated nerve fibers in the control group was 1164.73+71.39 in the proximal end, 532.78+100.68 in the distal end, 14.39+3.13 micron 2 in the distal end. There was no significant difference in the number of myelinated nerve fibers in the proximal end (P > 0.05). There was a significant difference in the number of myelinated nerve fibers at the heart end and the distal fiber cross-sectional area (P0.01).
3. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1523 (+ 0.0520) micron in the experimental group, and the diameter ratio of regenerated nerve fibers was 0.6364 (+ 0.1199) in the control group. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1017 (+ 0.0773) in the control group, and the diameter ratio of regenerated nerve fibers was 0.4969 (+ 0.1396) in the experimental group.
4. The wet weight of gastrocnemius muscle in experimental group was 805.91 65507 The nerve conduction velocity increased the number and cross-sectional area of the regenerated myelinated nerve fibers at the distal end of the anastomotic stoma, increased the wet weight of the gastrocnemius muscle and the cross-sectional area of the muscle fibers. FK1706 significantly promoted the nerve regeneration after nerve transplantation in rats.
The second part is an experimental study of FK1706 promoting nerve regeneration after nerve bypass grafting.
Objective:
Objective: To investigate the effect of FK1706 on nerve regeneration after nerve bypass grafting.
Twenty male SD rats, 250-300 g, were randomly divided into experimental group (FK1706 group) and control group. After anesthesia, the left radial nerve was reserved for 10 mm to expose the left sciatic nerve trunk. The left sciatic nerve trunk was clamped with a microscope needle-holding forceps for 10 seconds, and a 1.5 mm diameter window was opened on the epineurium near the forceps and 3 mm distal, respectively. End-to-side anastomosis. FK1706 (0.32mg/kg) was injected into the left lower limb muscle on the day after operation for 8 weeks. No intervention was given to the control group after nerve bypass grafting. The left sciatic nerve was electrophysiologically examined, the number and cross-sectional area of myelinated nerve fibers regenerated from the left sciatic nerve were measured, and the wet weight of left gastrocnemius muscle and muscle fibers were measured. Determination of sectional area.
Result:
1. The amplitude of compound motor action potential (CMAP) in the experimental group was 6.69 (+ 0.89mV) and that in the control group was 5.32 (+ 0.80mV) with a significant difference (P 0.01). The motor nerve conduction velocity (MNCV) in the experimental group was 5.98 (+ 1.14m/s) and that in the control group was 4.32 (+ 0.53m/s), with a significant difference (P 0.01).
2. The number of myelinated nerve fibers in experimental group was 1131.67 (+ 45.28) in the proximal end, 708.52 (+ 39.85) in the distal end and 20.68 (+ 6.95) in the distal end. The number of myelinated nerve fibers in control group was 1020.70 (+ 55.22) in the proximal end, 468.87 (+ 119.67) in the distal end, 13.29 (+ 2.39) in the distal end. There was no significant difference in the number of myelinated nerve fibers in the distal end (P > 0.05). There was a significant difference in the number of myelinated nerve fibers at the end and the distal fiber cross-sectional area (P0.01).
3. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1566 (+ 0.0659) micron and the diameter ratio of regenerated nerve fibers was 0.6269 (+ 0.1094) in the experimental group and 0.1022 (+ 0.0458) in the control group. The diameter ratio of regenerated nerve fibers was 0.5101 (+ 0.1294) in the experimental group and the difference was significant (P 0.05).
4. The wet weight of gastrocnemius muscle in experimental group was 753.54+105.91 mg, while that of control group was 598.02+65.02 mg (P 0.01).
Conclusion:
FK1706 can improve motor nerve conduction velocity, increase the number and cross-sectional area of regenerated myelinated nerve fibers, wet weight of gastrocnemius muscle and cross-sectional area of muscle fibers. FK1706 can promote nerve regeneration after nerve bypass grafting in rats.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R651.3
本文编号：2224728
[Abstract]:It is known that FK506 is an immunosuppressive agent which can significantly promote nerve regeneration, but its immunosuppressive effect limits its application in the repair of peripheral nerve injury. The regeneration of injured nerve graft and nerve bypass graft was studied with FK1706 after operation. The experiment was divided into the following two parts.
Part one experimental study on the effect of FK1706 on nerve regeneration after nerve transplantation
Objective:
Objective to investigate the effect of FK1706 on nerve regeneration after nerve transplantation.
Method:
20 male SD rats, 250-300 g, were randomly divided into experimental group (FK1706 group) and control group. After anesthesia, the left radial nerve was reserved for 10 mm and the left sciatic nerve trunk was exposed. At 0.5 cm below the piriformis muscle, the sciatic nerve of 8 mm length was resected with a knife blade, and the radial nerve was transplanted to the sciatic nerve. End-to-end anastomosis was performed at both ends of the sciatic nerve. FK1706 (0.32 mg/kg) was injected into the left lower limb for 8 weeks. The control group received no intervention after nerve transplantation and was fed routinely for 8 weeks.
Result:
1. The amplitude of compound motor action potential (CMAP) in the experimental group was 7.34 (+ 1.22 mV) and that in the control group was 6.07 (+ 1.18 mV) with a significant difference (P 0.05). The motor nerve conduction velocity (MNCV) in the experimental group was 6.07 (+ 0.95 m/s) and that in the control group was 3.78 (+ 0.46 m/s), with a significant difference (P 0.01).
2. The number of myelinated nerve fibers in the proximal end was 1199.53+113.73 in the experimental group, 701.17+78.40 in the distal end, 22.53+6.52 micron 2 in the distal end. The number of myelinated nerve fibers in the control group was 1164.73+71.39 in the proximal end, 532.78+100.68 in the distal end, 14.39+3.13 micron 2 in the distal end. There was no significant difference in the number of myelinated nerve fibers in the proximal end (P > 0.05). There was a significant difference in the number of myelinated nerve fibers at the heart end and the distal fiber cross-sectional area (P0.01).
3. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1523 (+ 0.0520) micron in the experimental group, and the diameter ratio of regenerated nerve fibers was 0.6364 (+ 0.1199) in the control group. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1017 (+ 0.0773) in the control group, and the diameter ratio of regenerated nerve fibers was 0.4969 (+ 0.1396) in the experimental group.
4. The wet weight of gastrocnemius muscle in experimental group was 805.91 65507 The nerve conduction velocity increased the number and cross-sectional area of the regenerated myelinated nerve fibers at the distal end of the anastomotic stoma, increased the wet weight of the gastrocnemius muscle and the cross-sectional area of the muscle fibers. FK1706 significantly promoted the nerve regeneration after nerve transplantation in rats.
The second part is an experimental study of FK1706 promoting nerve regeneration after nerve bypass grafting.
Objective:
Objective: To investigate the effect of FK1706 on nerve regeneration after nerve bypass grafting.
Twenty male SD rats, 250-300 g, were randomly divided into experimental group (FK1706 group) and control group. After anesthesia, the left radial nerve was reserved for 10 mm to expose the left sciatic nerve trunk. The left sciatic nerve trunk was clamped with a microscope needle-holding forceps for 10 seconds, and a 1.5 mm diameter window was opened on the epineurium near the forceps and 3 mm distal, respectively. End-to-side anastomosis. FK1706 (0.32mg/kg) was injected into the left lower limb muscle on the day after operation for 8 weeks. No intervention was given to the control group after nerve bypass grafting. The left sciatic nerve was electrophysiologically examined, the number and cross-sectional area of myelinated nerve fibers regenerated from the left sciatic nerve were measured, and the wet weight of left gastrocnemius muscle and muscle fibers were measured. Determination of sectional area.
Result:
1. The amplitude of compound motor action potential (CMAP) in the experimental group was 6.69 (+ 0.89mV) and that in the control group was 5.32 (+ 0.80mV) with a significant difference (P 0.01). The motor nerve conduction velocity (MNCV) in the experimental group was 5.98 (+ 1.14m/s) and that in the control group was 4.32 (+ 0.53m/s), with a significant difference (P 0.01).
2. The number of myelinated nerve fibers in experimental group was 1131.67 (+ 45.28) in the proximal end, 708.52 (+ 39.85) in the distal end and 20.68 (+ 6.95) in the distal end. The number of myelinated nerve fibers in control group was 1020.70 (+ 55.22) in the proximal end, 468.87 (+ 119.67) in the distal end, 13.29 (+ 2.39) in the distal end. There was no significant difference in the number of myelinated nerve fibers in the distal end (P > 0.05). There was a significant difference in the number of myelinated nerve fibers at the end and the distal fiber cross-sectional area (P0.01).
3. The myelin sheath thickness (mt) of regenerated myelinated nerve fibers was 0.1566 (+ 0.0659) micron and the diameter ratio of regenerated nerve fibers was 0.6269 (+ 0.1094) in the experimental group and 0.1022 (+ 0.0458) in the control group. The diameter ratio of regenerated nerve fibers was 0.5101 (+ 0.1294) in the experimental group and the difference was significant (P 0.05).
4. The wet weight of gastrocnemius muscle in experimental group was 753.54+105.91 mg, while that of control group was 598.02+65.02 mg (P 0.01).
Conclusion:
FK1706 can improve motor nerve conduction velocity, increase the number and cross-sectional area of regenerated myelinated nerve fibers, wet weight of gastrocnemius muscle and cross-sectional area of muscle fibers. FK1706 can promote nerve regeneration after nerve bypass grafting in rats.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R651.3
【共引文献】
相关期刊论文 前10条
1 曹川,李世荣,尹锐,孙志成,王珍祥,吴军;外用环孢素A对异体移植鼠耳郎格罕细胞的影响及延长存活的实验研究[J];重庆医学;2002年04期
2 常保国;武文臣;;前臂上段背侧锐器伤诊治失误原因分析[J];长治医学院学报;2011年03期
3 裴国献,朱立军;人肢体异体移植最新进展[J];当代医学;2001年07期
4 张少成,修先伦,黄平,李庆涛,姚依平;神经束间侧侧吻合治疗局限性痉挛性脑瘫[J];第二军医大学学报;2000年04期
5 魏在荣;孙广峰;唐修俊;王达利;王玉明;;尺动脉近侧穿支蒂前臂内侧皮神经营养血管皮瓣修复肘部皮肤缺损[J];第三军医大学学报;2010年06期
6 曲辉,陈绍宗,刘明锁,李跃军,李学拥;兔神经端侧吻合再生轴突荧光素双标记研究[J];第四军医大学学报;2000年12期
7 马忠立,裴国献,朱立军,石玉生,陈龙华,张敬良,王珂,陈滨,魏宽海;X线照射对同种异体肢体移植排斥反应的影响[J];第一军医大学学报;2002年06期
8 胡庆柳,邹飞,朴英杰;人发角蛋白导管修复周围神经缺损的实验研究[J];第一军医大学学报;2002年09期
9 王旭f;陈学英;殷少芳;李宝兴;赖炽香;;去细胞神经移植后再生神经纤维超微结构的观察[J];电子显微学报;2009年03期
10 伍成奇,黄立新,李志安,任书信,张自清;应用显微技术修复周围神经损伤[J];临床医学;2001年08期
本文编号：2224728
本文链接：https://www.wllwen.com/yixuelunwen/mazuiyixuelunwen/2224728.html
最近更新
教材专著
