雷公藤总生物碱对粘虫幼虫神经系统酶活性及神经递质含量的影响

发布时间：2018-09-07 14:29

【摘要】：【目的】明确雷公藤Tripterygium wilfordii Hook.f.生物碱对粘虫Mythimna separata(Walker)神经系统的影响,为阐明其杀虫作用机制提供依据。【方法】采用载毒叶片法测定粘虫5龄幼虫经雷公藤总生物碱处理后体内乙酰胆碱酯酶(ACh E)、Na+,K+-ATPase、Ca2+,Mg2+-ATPase、谷丙转氨酶(GPT)和谷氨酸脱羧酶(GAD)等重要神经系统酶活性及乙酰胆碱(ACh)、谷氨酸(Glu)和γ-氨基丁酸(GABA)等神经递质的含量。【结果】雷公藤总生物碱处理对粘虫5龄幼虫ACh E无明显影响,麻醉期处理粘虫幼虫体内ACh相对含量与同期对照无显著差异。处理粘虫幼虫在轻度麻醉期、深度麻醉期和复苏期体内GABA和Glu含量显著升高,GABA含量分别升高了89.86%,49.28%和20.29%,Glu含量分别升高了24.55%,23.33%和8.13%。处理粘虫幼虫GPT活性明显受到抑制,而GAD活性无明显变化。处理明显抑制粘虫幼虫头部Na+,K+-ATPase和Ca2+,Mg2+-ATPase活性,但对中肠两种ATPase活性影响不大。【结论】研究结果有助于了解雷公藤生物碱对昆虫神经系统的影响,也为进一步阐明其作用靶标奠定了基础。
[Abstract]:[objective] to clarify Tripterygium wilfordii Hook.f. of Tripterygium wilfordii Effects of alkaloids on Mythimna separata (Walker) nervous system of Myxomycetes To provide the basis for elucidating the insecticidal mechanism. [methods] to determine the acetylcholinesterase (ACh E), glutamyl transaminase (GPT) and glutamate removal in the body of the 5th instar larvae treated with total alkaloids of Tripterygium wilfordii by means of leaf method. Carboxylase (GAD) and other important nervous system enzymes, and the contents of neurotransmitters such as acetylcholine (ACh), glutamate (Glu) and 纬 -aminobutyric acid (GABA). [results] Total alkaloids of Tripterygium wilfordii had no significant effect on ACh E of 5th instar larvae. There was no significant difference in the relative content of ACh between the anaesthesia period and the control. The contents of GABA and Glu in the larvae of armyworm during mild anesthesia, deep anesthesia and resuscitation were increased by 89.86%, 49.28% and 20.29%, respectively, and the contents of GABA and Glu were increased by 24.553.33% and 8.13%, respectively. The activity of GPT was inhibited obviously, but the activity of GAD was not changed. Treatment significantly inhibited the activities of Na K ATPase and Ca2 mg 2 ATPase in the head of the larvae, but had little effect on the activities of ATPase in the midgut. [conclusion] the results of this study are helpful to understand the effect of tripterygium wilfordii alkaloids on the nervous system of insects. It also lays a foundation for further elucidation of the target.
【作者单位】：河南农业大学植物保护学院河南省新型农药创制与应用重点实验室;西北农林科技大学无公害农药研究服务中心陕西省生物农药工程技术研究中心;
【基金】：国家“十五”科技攻关项目(2004BA516A04) 公益性行业(农业)科研专项(200903052) 河南省重点科技攻关项目(082102350033)
【分类号】：S482.39

【参考文献】