七氟烷预处理对大鼠肺缺血再灌注损伤的保护作用

发布时间：2018-09-10 15:42

【摘要】：目的：探讨七氟烷预处理是否可以保护大鼠肺缺血再灌注损伤及其作用机制,从而为临床麻醉实施中麻醉药物的选择提供实验和理论依据。方法：成年雄性SD大鼠54只,体重为250-320g左右,采用随机数字表法,将其随机分为3组(n=18)假手术组(S组)：仅游离左肺门,但不阻断；肺缺血再灌注组(I/R组)：采用阻断左肺门45min后再恢复灌注的方法制备大鼠肺缺血再灌注损伤模型；七氟烷预处理组(SP组)：吸入浓度为2.1%的七氟烷30min后制备肺缺血再灌注模型。各组分别于再灌注30、60和120min时随机取6只大鼠,处死后取肺组织,测定其湿/干重比值(W/D比),采用比色法测定肺组织髓过氧化物酶(MPO)活性,采用RT-PCR测肺组织ACE mRNA的表达水平,光镜下观察肺组织病理变化。结果：与S组比较,I/R组和SP组再灌注各时间点肺组织W/D比、MPO活性和ACE mRNA表达水平升高,差异具有统计学意义(P0.05)；I/R组和SP组随着再灌注时间的延长肺组织W/D比、MPO活性、ACE mRNA表达水平增高,但在每个再灌注时间点,SP组的肺组织W/D比、MPO活性和ACE mRNA表达水平明显低于I/R组(P0.05)；SP组的肺组织损伤较I/R组减轻。结论：七氟烷预处理可能通过下调ACE mRNA的表达和抑制MPO活性从而减轻肺缺血再灌注损伤。
[Abstract]:Aim: to investigate whether sevoflurane pretreatment can protect lung ischemia-reperfusion injury and its mechanism in rats, and provide experimental and theoretical basis for the selection of anesthetic drugs in clinical anesthesia. Methods: 54 adult male SD rats, weighing about 250-320g, were randomly divided into 3 groups (n = 18): sham operation group (S group): only left hilum was free, but not blocked. Lung ischemia-reperfusion group (I / R group): the model of lung ischemia-reperfusion injury was established by blocking left pulmonary hilus 45min after reperfusion, and that of sevoflurane preconditioning group (SP group) was induced by inhalation of 2.1% sevoflurane 30min. In each group, 6 rats were randomly selected during reperfusion (30 ~ 60) and 120min. Lung tissues were killed and their wet / dry weight ratio (W / D) was measured. The activity of myeloperoxidase (MPO) in lung tissue was measured by colorimetric method, and the expression of ACE mRNA in lung tissue was measured by RT-PCR. The pathological changes of lung tissue were observed under light microscope. Results: compared with S group, the lung tissue WR activity and ACE mRNA expression level of I / R group and SP group were significantly higher than those of SP group (P0.05). The expression of W- / D and ACE mRNA in lung tissue of group I / R and SP were significantly lower than that of group I / R (P0.05) with the prolongation of reperfusion time, but the expression of W- / D was significantly lower than that of group I / R at each time point after reperfusion (P < 0.05), but the expression of W- / D was significantly lower than that of group I / R (P < 0.05). Lung tissue injury in SP group was lighter than that in I / R group. Conclusion: sevoflurane preconditioning may reduce lung ischemia-reperfusion injury by down-regulating the expression of ACE mRNA and inhibiting the activity of MPO.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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