草苁蓉提取物对油酸型大鼠急性呼吸窘迫综合征的保护作用
发布时间：2018-10-05 17:10
【摘要】：目的： 1.观察不同剂量的草苁蓉提取物对ARDS大鼠血清中TNF-α、IL-10水平的影响； 2.研究草苁蓉提取物对ARDS大鼠肺组织中磷酸化的p38MAPK (p-p38MAPK)蛋白的表达； 3.探讨草苁蓉提取物对ARDS的保护作用。 方法： 将50只平均体重为(200±20)g的SD雄性大鼠适应性饲养一周后随机分为5组,每组10只,分别为空白组、模型组、草苁蓉低剂量组、草苁蓉中剂量组、草苁蓉高剂量组。实验开始第1-6天每天按1ml/100g分别给空白组和模型组大鼠生理盐水灌胃,同时给草苁蓉低、中、高剂量3个组分别按125mg/kg、250mg/kg、500mg/kg剂量连续灌胃草苁蓉提取物。在实验第6天将所有动物行乌拉坦(1.0g/kg)腹腔麻醉后,空白组尾静脉注射生理盐水5ml/kg,模型组、草苁蓉低剂量组、草苁蓉中剂量组、草苁蓉高剂量组尾静脉注射油酸0.1ml/kg。3小时后,观察大鼠一般状态,处死大鼠。左肺计算肺组织湿/干重(W/D)比值；用HE染色法观察右肺组织病理变化；用ELISA法检测血清中TNF-α和IL-10的浓度；用免疫印迹法检测右肺组织中p-p38MAPK蛋白的表达。 结果： 1.与空白组比较,模型组大鼠左肺的W/D明显升高(P0.05)；与模型组比较,草苁蓉中、高剂量组大鼠左肺的W/D明显降低(P0.05)。 2.组织病理学HE染色结果显示：光镜下,空白组大鼠肺组织结构清晰,细支气管粘膜上皮完整,假复层柱状上皮细胞排列有序,肺泡腔内清晰,间隔完整无水肿,无明显炎性渗出；模型组大鼠肺组织结构不完整,且肺泡萎陷,肺泡腔内见以中性粒细胞为主的炎性细胞浸润,以及大量渗出性蛋白水肿液,肺间质毛细血管扩张、充血,肺泡间隔明显增厚、水肿；草苁蓉各剂量组大鼠肺组织结构基本完整,肺泡腔内水肿、中性粒细胞浸润以及肺泡间隔增厚较模型组减轻。草苁蓉高剂量组炎性病变较低、中剂量组轻。 3.与空白组比较,模型组大鼠血清中的TNF-α、IL-10浓度明显升高(P0.05)；与模型组比较,草苁蓉中、高剂量组大鼠的TNF-α、IL-10浓度明显降低(P0.05)。 4.空白组大鼠肺组织中p-p38MAPK蛋白的表达很弱,模型组中p-p38MAPK蛋白的表达明显增加,草苁蓉各剂量组中p-p38MAPK的表达较模型组明显降低。 结论： 草苁蓉提取物在ARDS发生时对正常肺组织有明显的保护作用。 1.草苁蓉提取物抑制血清中TNF-α、IL-10的表达； 2.草苁蓉提取物抑制肺组织中p-p38MAPK的表达,其原因可能与草苁蓉对MAPK通路的调节有关； 3.草苁蓉提取物为125-500mg/kg剂量之间时,在ARDS发生时对正常肺组织的保护作用与剂量关系成正比。
[Abstract]:Objective: 1. To observe the effect of different doses of Cistanche deserticola extract on the level of TNF- 伪 -IL-10 in serum of ARDS rats. To study the effect of Cistanche deserticola extract on the expression of phosphorylated p38MAPK (p-p38MAPK) protein in lung tissue of ARDS rats. To investigate the protective effect of Cistanche deserticola extract on ARDS. Methods: fifty male SD rats with average body weight of (200 卤20) g were randomly divided into 5 groups, 10 rats in each group, which were blank group, model group, Cistanche deserticola low dose group and middle dose group. High dose group of Cistanche deserticola. At the beginning of 1-6 days, the rats in the control group and the model group were given 1ml/100g daily with normal saline, while Cistanche deserticola was administered with low, medium and high doses of 125 mg / kg 250 mg / kg ~ 500 mg / kg Cistanche deserticola extract respectively by intragastric administration of Cistanche deserticola extract at the dose of 125 mg / kg ~ 250 mg 路kg ~ (-1) 路kg ~ (-1) ~ (-1). On the 6th day of the experiment, all the animals were anesthetized intraperitoneally with 1.0g/kg, the rats in the blank group were injected with saline 5 ml / kg via tail vein, the model group, the low dose group of Cistanche deserticola, the middle dose group of Cistanche deserticola, and the high dose group of Cistanche deserticola were injected with oleic acid 0.1ml/kg.3 hours later. The general state of the rats was observed and the rats were killed. The wet / dry weight (W / D) ratio of the left lung was calculated, the pathological changes of the right lung were observed by HE staining, the serum TNF- 伪 and IL-10 levels were detected by ELISA method, and the expression of p-p38MAPK protein in the right lung was detected by Western blotting. Results: 1. Compared with the blank group, the W / D of the left lung in the model group was significantly higher than that in the model group (P0.05); compared with the model group, the W / D of the left lung in the high dose group was significantly lower than that in the model group (P0.05). 2. The results of histopathological HE staining showed that the lung tissue structure was clear, the bronchiolar mucosal epithelium was intact, the pseudostratified columnar epithelial cells were arranged orderly, the alveolar cavity was clear and the septum was intact without edema. There was no obvious inflammatory exudation in the model group, the pulmonary tissue structure was incomplete and alveolar collapse was found in the model group, the infiltration of inflammatory cells with neutrophil in the alveolar cavity, as well as a large amount of exudative protein edema fluid, pulmonary interstitial capillary dilatation and hyperemia were observed in the alveolar cavity. The alveolar septum was thickened and edema, the lung tissue structure of Cistanche deserticola group was basically intact, the alveolar cavity edema, neutrophil infiltration and alveolar septal thickening were less than those in the model group. The inflammatory lesion of Cistanche deserticola in high dose group was lower than that in middle dose group. 3. Compared with the blank group, the serum TNF- 伪 -IL-10 concentration in the model group was significantly higher (P0.05); compared with the model group, the concentration of TNF- 伪 -IL-10 in the Cistanche deserticola group was significantly lower than that in the high dose group (P0.05). 4. The expression of p-p38MAPK protein in lung tissue of blank group was very weak, the expression of p-p38MAPK protein in model group was obviously increased, and the expression of p-p38MAPK in each dose group of Cistanche deserticola was significantly lower than that in model group. Conclusion: Cistanche deserticola extract has obvious protective effect on normal lung tissue during ARDS. 1. The extract of Cistanche deserticola inhibited the expression of TNF- 伪 -IL-10 in serum. The extract of Cistanche deserticola inhibited the expression of p-p38MAPK in lung tissue, which may be related to the regulation of MAPK pathway by Cistanche deserticola. 3. The protective effect of Cistanche deserticola extract on normal lung tissue was proportional to the dose when the dose of Cistanche deserticola was between the doses of 125-500mg/kg.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5
本文编号：2254189
[Abstract]:Objective: 1. To observe the effect of different doses of Cistanche deserticola extract on the level of TNF- 伪 -IL-10 in serum of ARDS rats. To study the effect of Cistanche deserticola extract on the expression of phosphorylated p38MAPK (p-p38MAPK) protein in lung tissue of ARDS rats. To investigate the protective effect of Cistanche deserticola extract on ARDS. Methods: fifty male SD rats with average body weight of (200 卤20) g were randomly divided into 5 groups, 10 rats in each group, which were blank group, model group, Cistanche deserticola low dose group and middle dose group. High dose group of Cistanche deserticola. At the beginning of 1-6 days, the rats in the control group and the model group were given 1ml/100g daily with normal saline, while Cistanche deserticola was administered with low, medium and high doses of 125 mg / kg 250 mg / kg ~ 500 mg / kg Cistanche deserticola extract respectively by intragastric administration of Cistanche deserticola extract at the dose of 125 mg / kg ~ 250 mg 路kg ~ (-1) 路kg ~ (-1) ~ (-1). On the 6th day of the experiment, all the animals were anesthetized intraperitoneally with 1.0g/kg, the rats in the blank group were injected with saline 5 ml / kg via tail vein, the model group, the low dose group of Cistanche deserticola, the middle dose group of Cistanche deserticola, and the high dose group of Cistanche deserticola were injected with oleic acid 0.1ml/kg.3 hours later. The general state of the rats was observed and the rats were killed. The wet / dry weight (W / D) ratio of the left lung was calculated, the pathological changes of the right lung were observed by HE staining, the serum TNF- 伪 and IL-10 levels were detected by ELISA method, and the expression of p-p38MAPK protein in the right lung was detected by Western blotting. Results: 1. Compared with the blank group, the W / D of the left lung in the model group was significantly higher than that in the model group (P0.05); compared with the model group, the W / D of the left lung in the high dose group was significantly lower than that in the model group (P0.05). 2. The results of histopathological HE staining showed that the lung tissue structure was clear, the bronchiolar mucosal epithelium was intact, the pseudostratified columnar epithelial cells were arranged orderly, the alveolar cavity was clear and the septum was intact without edema. There was no obvious inflammatory exudation in the model group, the pulmonary tissue structure was incomplete and alveolar collapse was found in the model group, the infiltration of inflammatory cells with neutrophil in the alveolar cavity, as well as a large amount of exudative protein edema fluid, pulmonary interstitial capillary dilatation and hyperemia were observed in the alveolar cavity. The alveolar septum was thickened and edema, the lung tissue structure of Cistanche deserticola group was basically intact, the alveolar cavity edema, neutrophil infiltration and alveolar septal thickening were less than those in the model group. The inflammatory lesion of Cistanche deserticola in high dose group was lower than that in middle dose group. 3. Compared with the blank group, the serum TNF- 伪 -IL-10 concentration in the model group was significantly higher (P0.05); compared with the model group, the concentration of TNF- 伪 -IL-10 in the Cistanche deserticola group was significantly lower than that in the high dose group (P0.05). 4. The expression of p-p38MAPK protein in lung tissue of blank group was very weak, the expression of p-p38MAPK protein in model group was obviously increased, and the expression of p-p38MAPK in each dose group of Cistanche deserticola was significantly lower than that in model group. Conclusion: Cistanche deserticola extract has obvious protective effect on normal lung tissue during ARDS. 1. The extract of Cistanche deserticola inhibited the expression of TNF- 伪 -IL-10 in serum. The extract of Cistanche deserticola inhibited the expression of p-p38MAPK in lung tissue, which may be related to the regulation of MAPK pathway by Cistanche deserticola. 3. The protective effect of Cistanche deserticola extract on normal lung tissue was proportional to the dose when the dose of Cistanche deserticola was between the doses of 125-500mg/kg.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5
【参考文献】
相关期刊论文 前10条
1 王秀丽;宋志坚;;草苁蓉研究进展[J];黑龙江科技信息;2009年20期
2 陆月明;油酸致急性肺损伤动物模型析评[J];中华急诊医学杂志;2005年01期
3 叶汉兰,潘可欣;中西医结合治疗成人呼吸窘迫综合征20例[J];北京中医药大学学报;1998年01期
4 刘旭;胡克;;亚低温对急性肺损伤大鼠NF-κB、TNF-α影响的研究[J];临床肺科杂志;2009年04期
5 邱海波,代静泓,燕艳丽,杨毅,周韶霞;肿瘤坏死因子和白细胞介素-1基因多态性与急性呼吸窘迫综合征的相关性研究[J];东南大学学报(医学版);2004年01期
6 周代星;祝伟;钟强;李树生;杨光田;;小潮气量通气对创伤性ARDS的疗效分析[J];内科急危重症杂志;2008年02期
7 薛嫱;王彤;田巍;倪莉;;MMP-9与TIMP-1在油酸制备大鼠急性肺损伤模型中的表达特点与临床意义探讨[J];首都医科大学学报;2010年06期
8 叶伶;金美玲;徐晓波;高磊;白春学;朱蕾;;急性肺损伤/急性呼吸窘迫综合征患者凝血纤溶系统的变化[J];复旦学报(医学版);2008年05期
9 孙仓,常桂英,张桂荣;草苁蓉现代研究进展[J];特产研究;1997年02期
10 杨树春,于海峰,王雪松,郭宝英,宋新生,李长河;草苁蓉资源的开发利用与保护[J];特种经济动植物;1998年01期
，本文编号：2254189
本文链接：https://www.wllwen.com/yixuelunwen/mazuiyixuelunwen/2254189.html
最近更新
教材专著
