经胃内镜及腹腔镜腹腔探查对雄兔急性炎性反应的影响

发布时间：2018-10-08 21:32

【摘要】：目的建立经胃内镜及腹腔镜腹腔探查的雄兔模型,对比两者对急性炎性反应的影响。方法雄性日本大耳白兔随机分为经胃内镜(N组,n=8)及腹腔镜(L组,n=8)腹腔探查组,假手术组(S组)5只,酶联免疫吸附试验(ELISA)检测术前、术后4、24及72 h血清肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6及IL-10的水平。结果动物模型建立成功,N组手术时间较L组长,前者出现胃造口漏1例;TNF-α、IL-6及IL-10水平在术前各组间及S组麻醉前后差异无统计学意义;N、L组TNF-α术后4 h明显上升,N组上升滞后,但维持时间长,术后4 h L组高于N组,术后24 h相反;N、L组IL-6术后24 h明显上升,N组术后72 h下降,L组不明显,术后4 h L组低于N组,术后72 h相反;N组术后IL-10较术前变化不明显,L组术后24 h明显下降,持续至72h,术后24及72 h N组均高于L组。结论经胃内镜及腹腔镜腹腔探查的雄兔模型是可行的,前者耗时较长,胃造口漏是严重的并发症;两者均引发全身促炎反应,但出现及维持时间存在差异,前者抗炎反应变化不明显,后者术后24及72 h持续低下。
[Abstract]:Objective to establish a male model of intraperitoneal exploration by endoscopy and laparoscopy, and to compare the effects of the two models on acute inflammatory reaction. Methods male Japanese large ear white rabbits were randomly divided into two groups: intraperitoneal exploration group (group N) and laparoscopy (group L), and sham operation group (group S). (ELISA) was detected by Elisa before operation. Serum levels of tumor necrosis factor- 伪 (TNF- 伪), interleukin (IL) 6 and IL-10 at 24 and 72 hours after operation. Results the operation time of group N was longer than that of group L, the level of TNF- 伪 IL-6 and IL-10 in 1 case with gastric leakage was not significantly different between each group before operation and before and after anesthesia in group S. The increase of TNF- 伪 in group N was significantly higher than that in group L 4 h after operation. However, the maintenance time was longer, 4 h after operation in L group was higher than that in N group, on the contrary, 24 h after operation, the IL-6 in group N increased significantly, and the decrease of IL-6 in group N at 72 h after operation was not obvious, but that in group L was lower than that in group N at 4 h after operation. On the contrary, the IL-10 of group N was significantly lower than that of group L at 24 h after operation, and lasted for 72 h, and that of group N was higher than that of group L at 24 h and 72 h postoperatively. Conclusion it is feasible to explore the male rabbit model by gastroendoscopy and laparoscopy, the former takes a long time and the gastric leakage is a serious complication, both of them cause systemic inflammatory reaction, but there are differences in the time of appearance and maintenance. The former had no obvious change in anti-inflammatory reaction, while the latter had a low level at 24 and 72 hours after operation.
【作者单位】：南昌大学第二附属医院肝胆外科;
【基金】：江西省卫生厅科技计划项目(编号:20094005)
【分类号】：R57

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