邵氏“五针法”对哮喘模型大鼠a-SMA及FN表达影响的实验研究
发布时间：2018-10-14 20:12
【摘要】：目的 本课题通过针刺哮喘模型大鼠“肺俞”、“大椎”、“风门”穴，观察邵氏“五针法”对各组哮喘模型大鼠肺组织和血清中平滑肌肌动蛋白-a（a-SMA）及纤维连接蛋白（FN）表达的影响以及对肺组织病理变化的影响，探讨邵氏“五针法”治疗哮喘的机理及其对哮喘模型大鼠气道重塑的作用机制，为针灸治疗哮喘提供科学的实验依据。 方法 选取健康雄性清洁级SD大鼠40只（体重140±20g），随机分成空白对照组、模型对照组、药物治疗组和针刺治疗组，每组10只。采用腹腔注射卵蛋白（OVA）致敏，雾化吸入1%的OVA溶液（每日1次，共7次）激发建立哮喘大鼠模型。针刺治疗组于每日激发后针刺哮喘大鼠“肺俞”(双)、“大椎”、“风门”(双)穴治疗；药物治疗组于每日激发后腹腔注射0.05%地塞米松磷酸钠溶液治疗；模型对照组激发后不做特殊处理；空白对照组腹腔注射及雾化吸入时，用生理盐水代替卵蛋白进行操作。末次治疗后6h内水合氯醛麻醉大鼠，取眼球血后脱颈椎处死大鼠，取右中下肺组织，石蜡包埋，制备病理切片，行苏木精-伊红（HE）染色及a-SMA、FN肺组织免疫组化染色和血清ELISA检测，观察大鼠肺组织形态学改变以及a-SMA、FN在哮喘模型大鼠肺组织和血清中的表达。 结果 1.各组大鼠行为体征观察 模型对照组大鼠与空白对照组比较，出现喷嚏，呛咳，流涕，喘息，呼吸急促，精神不振，反应迟钝；空白对照组大鼠行动敏捷，未见异常；药物治疗组、针刺治疗组大鼠喷嚏，呛咳，喘息等症状较模型对照组明显减轻，反应灵敏。 2.各组大鼠肺组织形态学观察 模型对照组大鼠气道黏膜上皮受损脱落，黏膜下层和平滑肌层增生、变形；气道壁增厚，管腔变窄，管腔内大量分泌物；肺间质大量炎症细胞浸润，肺泡壁破裂变形，肺泡间隔结构紊乱；血管增生，血管管壁增厚。药物治疗组、针刺治疗组气道结构基本完整，肺间质炎症细胞浸润较少，肺组织结构基本正常。空白对照组大鼠气道结构完整，无明显脱落和变形，肺间质未见炎症细胞浸润，肺组织形态结构正常。 3.各组大鼠肺组织a-SMA的比较 空白对照组大鼠肺组织中少见胞浆呈棕黄色染色的a-SMA阳性细胞；模型对照组大鼠肺组织内阳性表达明显增多，与空白对照组比较，差异具有统计学意义(P＜0.01)；针刺治疗组及药物治疗组与模型对照组比较，肺组织内阳性表达明显减少，差异具有统计学意义(P＜0.01)，但仍高于空白对照组；针刺治疗组与药物治疗组比较，差异具有统计学意义(P＜0.01)。 4.各组大鼠肺组织FN的比较 空白对照组大鼠肺组织中少见胞浆呈棕黄色染色的FN阳性细胞；模型对照组大鼠肺组织内阳性表达明显增多，与空白对照组比较，，差异具有统计学意义(P＜0.01)；针刺治疗组及药物治疗组与模型对照组比较，肺组织内阳性表达明显减少，差异具有统计学意义(P＜0.01)，但仍高于空白对照组；针刺治疗组与药物治疗组比较，差异具有统计学意义(P＜0.01)。 5.各组大鼠血清a-SMA的比较 模型对照组与空白对照组相比，大鼠血清中a-SMA浓度显著增高，差异具有统计学意义(P＜0.01)；针刺治疗组及药物治疗组与模型对照组比较，a-SMA浓度明显降低，差异具有统计学意义(P＜0.01)，但仍高于空白对照组；针刺治疗组与药物治疗组比较，差异具有统计学意义(P＜0.01)。 6.各组大鼠血清FN的比较 模型对照组与空白对照组相比，大鼠血清中FN浓度显著增高，差异具有统计学意义(P＜0.01)；针刺治疗组及药物治疗组与模型对照组比较，FN浓度明显降低，差异具有统计学意义(P＜0.01)，但仍高于空白对照组；针刺治疗组与药物治疗组比较，差异具有统计学意义(P＜0.01)。 结论 1.邵氏“五针法”能够有效改善哮喘模型大鼠的症状和行为体征。 2.邵氏“五针法”能够有效改善哮喘模型大鼠的肺组织形态学改变。 3.邵氏“五针法”能够降低哮喘模型大鼠肺组织中a-SMA和FN的阳性表达。 4.邵氏“五针法”能够降低哮喘模型大鼠血清中a-SMA和FN的阳性表达，且二者在各组哮喘模型大鼠肺组织和血清中的表达具有一致性，并呈正相关。 邵氏“五针法”能明显降低哮喘模型大鼠肺组织和血清中a-SMA及FN的阳性表达，改善气道重塑，说明降低a-SMA、FN的阳性表达是邵氏“五针法”治疗哮喘的作用途径之一。
[Abstract]:Purpose The objective of this study is to adopt acupuncture and asthma model. Model Rats lung "," Big Vertebra "," Air Door "Point, View Czechsler's Five Effect of Acupuncture on the Expression of Smooth Muscle Actin-a (a-SMA) and Fibronectin (FN) in Lung Tissue and Serum of Rats with Asthma Model Discussion on the Five-year-old Five-year-Five-Year Plan The mechanism of acupuncture therapy for asthma and the mechanism of airway remodeling in asthma model rats. It provides scientific experiments for acupuncture and moxibustion treatment of asthma. Basis Methods Forty SD rats were randomly divided into control group, model control group, drug treatment group and acupuncture treatment group. Treatment group, 10 rats in each group, using intraperitoneal injection of ovalbumin (OVA) to induce sensitivity, atomizing inhalation of 1% solution (once daily, 7 times) Model of asthma rats was established. The acupuncture treatment group was administered daily. Post-excitation needle Staggle asthma rat's lungs" (double)," Dazhui "," air door "(double) acupoint treatment; drug treatment group injected intraperitoneally with 0.05% dexamethasone sodium phosphate solution after daily excitation; no special treatment after excitation of the model control group; when the blank control group was injected and atomized in the abdominal cavity, saline was used for the treatment. The ovalbumin was operated at the end of last treatment. After the last treatment, the rats were anesthetized with chloral hydrate, the rats were sacrificed after taking the eyeball blood, the lung tissue in the right was removed, paraffin was embedded, pathological sections were prepared, hematoxylin-eosin (HE) staining, and a-SMA, FN lung tissue immunohistochemical staining and blood were prepared. The morphological changes of lung tissue of rats and the pulmonary groups of a-SMA and FN in asthmatic rats were observed by ELISA. Weaving and Expression in serum. Results 1. The rats in the control group were compared with the blank control group in the control group of the signs of behavioral signs in each group. The rats were quick and abnormal in action; the drug treatment group, the acupuncture group, the acupuncture group, the group of acupuncture and treatment group, the chronic cough, the wheezing, etc. The symptoms were significantly reduced compared with the model control group, It should be sensitive. 2. The model of lung tissue morphology in each group of rats was observed in the control group, and the mucosal epithelium of the airway was damaged and shed, the submucosa and smooth muscle layer proliferated and deformed, the airway wall was thickened, the tube cavity became narrow, and the tube cavity was large. Keywords secretion; pulmonary interstitial massive inflammatory cell infiltration; alveolar wall rupture Deformation, alveolar space structure disorder, vascular proliferation, vascular wall thickening, drug treatment group, acupuncture treatment group airway structure basically completed There was little infiltration of inflammatory cells in interstitial inflammatory cells, and the structure of lung tissue was basically normal. No inflammatory cell infiltration, lung tissue was found in the interstitial lung Morphologic structure was normal. 3. Compared with the control group of lung tissue a-SMA in each group, the rare cells in the lung tissues of the rats were brown-yellow stained a-SMA positive cells, and the positive expression in the lung tissues of the model control group was obvious. Compared with the blank control group, the difference was statistically significant (P <0.01). Compared with the model control group, the positive expression in the lung tissue was significantly reduced and the difference was statistically significant (P <0.01), but still higher than that of the blank control group. Comparison between treatment group and drug treatment group, difference There was a statistically significant difference (P <0.01). 4. Compared with the control group of lung tissue FN in each group, there were no FN positive cells stained with brown yellow in the lung tissues of the control group; the model control group was in the lung tissue of the rats. There was a significant increase in the positive expression in the lung tissue compared with the control group (P <0.01). Compared with the control group, the positive expression in the lung tissue was significantly reduced, and the difference was statistically significant (P <0.01), but still higher than that in the blank group. Control group; acupuncture treatment group and drug treatment group ratio Compared with the control group, the levels of a-SMA in the serum of rats were significantly higher than that in the control group, and the difference was statistically significant. (P <0.01), the concentration of a-SMA decreased significantly in the acupuncture group and the drug treatment group compared with the model control group (P <0.01). but still higher than blank control group; acupuncture treatment Compared with the control group, the concentration of FN in the serum of rats was significantly higher than that in the control group. The difference was statistically significant (P <0.01); in acupuncture treatment group and drug treatment group, FN concentration decreased significantly compared with the model control group, and the difference was statistically significant (P <0.01). 0. 01), but still higher than empty Comparison and difference between acupuncture treatment group and drug treatment group. with statistics Learning significance (P <0.01)............................................................... the law" can effective improvement of nasal polyps Symptoms and signs of behavior in asthma model rats. effective improvement Asthma model is large Morphological change of lung tissue in rats. The five-stitch "five stitches" can reduce the positive expression of a-SMA and FN in the lung tissues of asthmatic model rats. Law can reduce the temperature and humidity The expression of a-SMA and FN in serum of asthmatic model rats was consistent and positively correlated with the expression of a-SMA and FN in asthmatic model rats. Wu's Five-pin The method can obviously reduce the asthma model
【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R245
[Abstract]:Purpose The objective of this study is to adopt acupuncture and asthma model. Model Rats lung "," Big Vertebra "," Air Door "Point, View Czechsler's Five Effect of Acupuncture on the Expression of Smooth Muscle Actin-a (a-SMA) and Fibronectin (FN) in Lung Tissue and Serum of Rats with Asthma Model Discussion on the Five-year-old Five-year-Five-Year Plan The mechanism of acupuncture therapy for asthma and the mechanism of airway remodeling in asthma model rats. It provides scientific experiments for acupuncture and moxibustion treatment of asthma. Basis Methods Forty SD rats were randomly divided into control group, model control group, drug treatment group and acupuncture treatment group. Treatment group, 10 rats in each group, using intraperitoneal injection of ovalbumin (OVA) to induce sensitivity, atomizing inhalation of 1% solution (once daily, 7 times) Model of asthma rats was established. The acupuncture treatment group was administered daily. Post-excitation needle Staggle asthma rat's lungs" (double)," Dazhui "," air door "(double) acupoint treatment; drug treatment group injected intraperitoneally with 0.05% dexamethasone sodium phosphate solution after daily excitation; no special treatment after excitation of the model control group; when the blank control group was injected and atomized in the abdominal cavity, saline was used for the treatment. The ovalbumin was operated at the end of last treatment. After the last treatment, the rats were anesthetized with chloral hydrate, the rats were sacrificed after taking the eyeball blood, the lung tissue in the right was removed, paraffin was embedded, pathological sections were prepared, hematoxylin-eosin (HE) staining, and a-SMA, FN lung tissue immunohistochemical staining and blood were prepared. The morphological changes of lung tissue of rats and the pulmonary groups of a-SMA and FN in asthmatic rats were observed by ELISA. Weaving and Expression in serum. Results 1. The rats in the control group were compared with the blank control group in the control group of the signs of behavioral signs in each group. The rats were quick and abnormal in action; the drug treatment group, the acupuncture group, the acupuncture group, the group of acupuncture and treatment group, the chronic cough, the wheezing, etc. The symptoms were significantly reduced compared with the model control group, It should be sensitive. 2. The model of lung tissue morphology in each group of rats was observed in the control group, and the mucosal epithelium of the airway was damaged and shed, the submucosa and smooth muscle layer proliferated and deformed, the airway wall was thickened, the tube cavity became narrow, and the tube cavity was large. Keywords secretion; pulmonary interstitial massive inflammatory cell infiltration; alveolar wall rupture Deformation, alveolar space structure disorder, vascular proliferation, vascular wall thickening, drug treatment group, acupuncture treatment group airway structure basically completed There was little infiltration of inflammatory cells in interstitial inflammatory cells, and the structure of lung tissue was basically normal. No inflammatory cell infiltration, lung tissue was found in the interstitial lung Morphologic structure was normal. 3. Compared with the control group of lung tissue a-SMA in each group, the rare cells in the lung tissues of the rats were brown-yellow stained a-SMA positive cells, and the positive expression in the lung tissues of the model control group was obvious. Compared with the blank control group, the difference was statistically significant (P <0.01). Compared with the model control group, the positive expression in the lung tissue was significantly reduced and the difference was statistically significant (P <0.01), but still higher than that of the blank control group. Comparison between treatment group and drug treatment group, difference There was a statistically significant difference (P <0.01). 4. Compared with the control group of lung tissue FN in each group, there were no FN positive cells stained with brown yellow in the lung tissues of the control group; the model control group was in the lung tissue of the rats. There was a significant increase in the positive expression in the lung tissue compared with the control group (P <0.01). Compared with the control group, the positive expression in the lung tissue was significantly reduced, and the difference was statistically significant (P <0.01), but still higher than that in the blank group. Control group; acupuncture treatment group and drug treatment group ratio Compared with the control group, the levels of a-SMA in the serum of rats were significantly higher than that in the control group, and the difference was statistically significant. (P <0.01), the concentration of a-SMA decreased significantly in the acupuncture group and the drug treatment group compared with the model control group (P <0.01). but still higher than blank control group; acupuncture treatment Compared with the control group, the concentration of FN in the serum of rats was significantly higher than that in the control group. The difference was statistically significant (P <0.01); in acupuncture treatment group and drug treatment group, FN concentration decreased significantly compared with the model control group, and the difference was statistically significant (P <0.01). 0. 01), but still higher than empty Comparison and difference between acupuncture treatment group and drug treatment group. with statistics Learning significance (P <0.01)............................................................... the law" can effective improvement of nasal polyps Symptoms and signs of behavior in asthma model rats. effective improvement Asthma model is large Morphological change of lung tissue in rats. The five-stitch "five stitches" can reduce the positive expression of a-SMA and FN in the lung tissues of asthmatic model rats. Law can reduce the temperature and humidity The expression of a-SMA and FN in serum of asthmatic model rats was consistent and positively correlated with the expression of a-SMA and FN in asthmatic model rats. Wu's Five-pin The method can obviously reduce the asthma model
【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R245
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