肿节风在放射性肺损伤中的防护作用

发布时间：2018-10-19 19:53

【摘要】：目的:探讨肿节风对60COγ射线照射后在小型猪放射性肺损伤中的防护作用和机制。方法:60头雄性巴马小型猪随机分为正常组、照射组和肿节风组,在麻醉条件下对动物行右胸单次15 Gy的照射,正常组不予照射。肿节风组于放疗前1周开始经口腔给予肿节风配方颗粒溶液(30 mg·kg-1),正常组和单照组给予等量的生理盐水,直至实验结束。在照射后4,8,12,24周,从3个组中分别随机取5头小型猪采集右肺组织,行羟脯氨酸测定检测肺组织中胶原含量;免疫印迹检测转化生长因子-β1(TGF-β1),基质金属蛋白酶-2(MMP-2)和MMP-9的蛋白表达;明胶酶谱法测定MMP-2和MMP-9的酶活性。结果:在照射后不同时间点,照射组中羟脯氨酸含量,TGF-β1,MMP-2和MMP-9的蛋白表达,MMP-2,MMP-9的酶活性较正常组明显升高(P0.05);与照射组比较,肿节风组的羟脯氨酸含量,TGF-β1,MMP-2和MMP-9的蛋白表达,MMP-2,MMP-9的酶活性明显降低,差异具有统计学意义(P0.05)。结论:肿节风可通过降低TGF-β1,MMP-2和MMP-9的蛋白表达和后者的酶活性,减缓放射性肺纤维化的发展,从而发挥放射防护作用。
[Abstract]:Objective: to investigate the protective effect and mechanism of TJE on radiation induced lung injury in miniature pigs after 60CO 纬-irradiation. Methods: 60 male Bama miniature pigs were randomly divided into three groups: normal group, irradiation group and swollen Jiefeng group. The animals were exposed to the right chest for 15 Gy under anesthesia, but the normal group was not irradiated. One week before radiotherapy, the patients in the control group were given the same amount of normal saline solution in oral cavity (30 mg kg-1), and the control group and the single irradiation group were given the same amount of normal saline until the end of the experiment. At 24 weeks after irradiation, the right lung tissues were collected from 5 miniature pigs randomly, and the collagen content in lung tissue was measured by hydroxyproline assay, and the protein expression of transforming growth factor 尾 1 (TGF- 尾 1), matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected by Western blotting. The enzyme activities of MMP-2 and MMP-9 were determined by gelatinase method. Results: at different time points after irradiation, the content of hydroxyproline, the protein expression of TGF- 尾 1, MMP-2 and MMP-9, and the activity of MMP-2,MMP-9 in the irradiated group were significantly higher than those in the normal group (P0.05). The content of hydroxyproline, the protein expression of TGF- 尾 1, MMP-2 and MMP-9, and the activity of MMP-2,MMP-9 were significantly decreased in the control group (P0.05). Conclusion: TJD can slow down the development of radiation-induced pulmonary fibrosis by decreasing the protein expression of TGF- 尾 _ 1-MMP-2 and MMP-9 and the enzyme activity of the latter, thus exerting the radiation-protective effect.
【作者单位】：广西医科大学第一附属医院;广西壮族自治区妇幼保健院;
【基金】：广西科技攻关项目(1140003A-24)
【分类号】：R285.5

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