不同麻醉状态下Wistar大鼠图形视觉诱发电位稳定性研究

发布时间：2018-10-25 09:59

【摘要】：目的探索不同麻醉状态对Wistar大鼠图形视觉诱发电位(pattern visual evoked potential,PVEP)稳定性的影响。方法 40只8~10周龄Wistar大鼠,麻醉后于初级视皮层对应位置硬脑膜表面埋置记录电极,于鼻骨处埋置参考电极。对麻醉状态下的大鼠采用时间频率为2 Hz和空间频率为0.04 c·d~(-1)的黑白翻转棋盘格刺激,记录相同时间段内的PVEP及基线脑电(electroencephalogram,EEG)。根据EEG功率频谱分析将麻醉分为多个阶段,比较不同阶段大鼠PVEP振幅及峰值期的稳定性。结果麻醉状态下大鼠的EEG可以根据功率频谱图分为delta波为主的慢波睡眠期(0.1~3.0 Hz)、过渡期(3.1~4.4Hz)、theta波为主的浅睡眠期(4.5~6.0 Hz)。三个阶段的PVEP振幅分别为(20.55±8.32)μV、(32.25±4.86)μV、(28.12±4.79)μV,振幅的变异系数分别为40.49%、15.08%、17.04%。结论不同的麻醉状态下记录到的PVEP的振幅及峰值期稳定性不同。记录麻醉动物的PVEP时,应当同时监测实验动物的EEG活动情况,并且建议在EEG活动表现为浅麻醉状态时进行。
[Abstract]:Objective to explore the effects of different anesthetic states on the stability of graphic visual evoked potential (pattern visual evoked potential,PVEP) in Wistar rats. Methods 40 8-week-old Wistar rats were anesthetized. The recording electrodes were implanted on the surface of the dura mater at the corresponding position of the primary visual cortex and the reference electrodes were placed at the nasal bone. In anesthetized rats, PVEP and baseline EEG (electroencephalogram,EEG) were recorded by black-and-white flip checkerboard stimulation with time frequency of 2 Hz and spatial frequency of 0.04 c d-1. According to EEG power spectrum analysis, anesthesia was divided into several stages, and the stability of PVEP amplitude and peak period in different stages were compared. Results the EEG of anesthetized rats could be divided into slow wave sleep period (0.1 ~ 3.0 Hz), transition period) and light sleep period (4.5 ~ 6.0 Hz).) based on power spectrum. The amplitudes of PVEP in the three stages were (20.55 卤8.32) 渭 V, (32.25 卤4.86) 渭 V, (28.12 卤4.79) 渭 V, respectively. The coefficients of variation of the amplitudes were 40.49 and 15.08 渭 V, respectively. Conclusion the amplitude and peak period stability of PVEP recorded under different anesthetic conditions are different. When recording the PVEP of anesthetized animals, the EEG activity of laboratory animals should be monitored at the same time, and it is suggested that the EEG activities should be performed when the EEG activities show superficial anaesthesia.
【作者单位】：天津市人民医院眼科;天津市眼科医院;
【基金】：国家自然科学基金资助(编号:81100694,81371049)~~
【分类号】：R339.14

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