线粒体保护剂对异氟醚麻醉老年小鼠海马线粒体功能和突触可塑性的影响

发布时间：2018-11-20 10:21

【摘要】：目的观察线粒体保护剂SS-31对异氟醚麻醉老年小鼠线粒体功能和突触可塑性的影响。方法老年雄性C57BL/6小鼠48只随机均分为四组(n=12):氧气+生理盐水组(CN组)、氧气+SS-31组(CS组)、异氟醚+生理盐水组(IN组)和异氟醚+SS-31组(IS组)。根据分组,于氧气或异氟醚吸入前30min腹腔注射等容生理盐水(CN、IN组)或SS-31(5mg/kg,CS、IS组),于气体吸入2h后取小鼠海马组织。提取线粒体检测电子传递链复合物Ⅰ~Ⅳ酶活性、三磷酸腺苷(ATP)和线粒体膜电位(MMP)水平,组织匀浆检测突触可塑性相关蛋白突触蛋白1(synapsin-1)、突触后密度蛋白95(PSD-95)、NMDA受体2A(NMDAR2A)、NMDAR2B、钙调蛋白依赖的蛋白激酶Ⅱα(CaMKⅡα)和CaMKⅡβ的含量。结果与IN组比较,IS组复合物Ⅰ酶活性、ATP和MMP水平升高,synapsin-1和PSD-95含量增加,NMDAR2B、CaMKⅡα和CaMKⅡβ含量降低(P0.05),而复合物Ⅱ~Ⅳ酶活性及NMDAR2A含量差异无统计学意义。结论线粒体保护剂SS-31可改善异氟醚麻醉所致老年小鼠海马线粒体及神经元突触可塑性损伤。
[Abstract]:Aim to observe the effects of mitochondrial protectant SS-31 on mitochondrial function and synaptic plasticity in aged mice anesthetized with isoflurane. Methods 48 old male C57BL/6 mice were randomly divided into four groups: oxygen saline group (CN group), oxygen SS-31 group (CS group), isoflurane saline group (IN group) and isoflurane SS-31 group (IS group). According to the group, 30min was injected intraperitoneally with isovolumic saline (CN,IN group) or SS-31 (5mg / kg CSIS group) before inhalation of oxygen or isoflurane. Mitochondria were extracted to detect the enzyme activity of electron transfer chain complex 鈪，

本文编号：2344647