SD大鼠股骨骨折合并脊髓损伤的实验性研究

发布时间：2018-12-06 14:19

【摘要】：目的：建立一种既能成功维持长时间存活,并符合临床特征,又简便易行的实验动物模型。方法：将48只SD雄性大鼠随即分为单纯股骨骨折组(A组,24只)、股骨骨折合并脊髓损伤组(B组,24只),股骨骨折模型通过双侧小切口开放截骨术造成股骨中端横行骨折并植入内固定,参考Allen法,使用自制打击装置造成大鼠T10段脊髓急性挫伤性损伤,将骨折模型和脊髓损伤模型两种方法融合制造股骨骨折合并脊髓损伤模型,分别在相同条件下饲养4周,对两组SD大鼠在制模成功后不同时间点进行大体观察、4周时观察骨折断端愈合情况,观察其并发症,并计算造模成功率。结果与结论：术后骨折合并脊髓损伤动物模型均成活,双下肢感觉及运动功能丧失,但可利用双上肢缓慢匍匐向前移动,术后前3天进食少,活动少,夜间采用尾端悬吊后,骨折患肢未出现缺血坏死,到达第四周时,A组除麻醉死亡一只外均成活,B组共死亡4只,成活率为83.33%,髓腔内固定未见脱出,此时A、B组骨折断端均有连续骨痂生成,从体积上来看,B组骨痂组织明显大于A组。证实将改良Allen法与小切口股骨外侧开放截骨法结合后,简单易行,可以成功制作股骨骨折合并脊髓损伤动物模型,采用术后特殊护理,可以成功让该模型成活至第4周。
[Abstract]:Objective: to establish an experimental animal model which can successfully maintain long-time survival, accord with clinical characteristics and be easy to carry out. Methods: Forty-eight male SD rats were randomly divided into group A (n = 24) and group B (n = 24). The fracture model of femur was made by open bilateral small incision osteotomy and internal fixation was implanted. Referring to Allen method, the acute contusion injury of T10 segment spinal cord in rats was caused by self-made hitting device. The fracture model and spinal cord injury model were fused to make the femur fracture combined with spinal cord injury model. The two groups of SD rats were fed under the same conditions for 4 weeks. The two groups of SD rats were observed at different time points after the model making. At 4 weeks, the healing of fracture end was observed, the complications were observed, and the success rate of modeling was calculated. Results and conclusion: all the animal models of fracture complicated with spinal cord injury survived, the sensory and motor functions of both lower limbs were lost, but the upper limbs could be slowly creeping forward, the food intake was less and the activity was less in the first three days after operation, and the tail was suspended at night. There was no ischemic necrosis in the fractured limbs. At the end of the week, all but one of the injured limbs survived in group A, 4 in group B, with a survival rate of 83.33, and no prolapse of intramedullary fixation. Continuous callus formation was found at the fracture end in group B, and the callus tissue in group B was significantly larger than that in group A in volume. It was proved that the combination of the modified Allen method and the small incision open osteotomy of the lateral femur was simple and easy, and the animal model of femoral fracture with spinal cord injury could be made successfully. The special nursing care after operation could successfully keep the model alive until the 4th week.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R683.42;R651.2

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