盐酸羟考酮超前镇痛对福尔马林致痛模型大鼠疼痛行为和大鼠血液NO及P物质浓度的影响

发布时间：2018-12-14 04:00

【摘要】：通过刺激前应用盐酸羟考酮观察福尔马林致痛模型大鼠的镇痛效果和血液中NO及P物质浓度的变化,探讨盐酸羟考酮超前镇痛的作用机制。选取健康成年Wistar大鼠32只,雌雄各半,体质量250~300 g,随机分为4组(每组8只),正常空白对照组(A组)腹腔注射0.9%氯化钠溶液2 m L;福尔马林模型组(B组)用微量注射器抽取5%福尔马林100μL注入大鼠右侧后爪掌心皮下致痛;盐酸羟考酮致痛前给药组(C组),福尔马林致痛前30 min腹腔注射盐酸羟考酮0.2 mg·kg-1;盐酸羟考酮致痛后给药组(D组),福尔马林致痛后30 min腹腔注射盐酸羟考酮0.2 mg·kg-1。致痛后1,10,20,30,45,60,90 min观察动物疼痛行为改变,以累积疼痛评分评定疼痛行为。致痛后90 min用水合氯醛麻醉大鼠,心脏采血3 m L测定其NO及P物质浓度的变化。根据大鼠行为学改变及疼痛评分的结果显示,本试验造模成功。疼痛评分值:致痛后10 min,C组比B组明显减小(P0.05);60~90 min,C、D组与B组比较明显减小(P0.05)。血液标本中NO浓度C、D组与B组比较明显降低,差异有统计学意义(P0.01),C组和D组比较,差异也有统计学意义(P0.05);血液中P物质浓度C、D组与B组比较明显降低,差异也有统计学意义(P0.001),C组和D组比较,差异也有统计学意义(P0.05)。结果表明,大鼠福尔马林致痛模型致痛前和致痛后30 min腹腔注射盐酸羟考酮均有明显的镇痛作用,镇痛效果与降低血液中NO及P物质的浓度相关,二者比较,致痛前30min给药即超前镇痛作用更显著。
[Abstract]:The analgesic effect of formalin induced pain model rats and the changes of NO and substance P concentrations in blood were observed before stimulation, and the mechanism of preemptive analgesia with hydroxycodone hydrochloride was investigated. Thirty-two healthy adult Wistar rats were randomly divided into 4 groups (8 rats in each group) and 0.9% sodium chloride solution (2 mL) intraperitoneally in normal control group (group A). In group B, 5% formalin 100 渭 L was injected into the right posterior paw of rats to cause subcutaneous pain. Group C (group C) before pain induced by hydroxycodone hydrochloride, 0.2 mg kg-1; intraperitoneal injection of hydroxycodone hydrochloride 30 min before formalin induced pain Group D (group D) after pain induced by hydroxycodone hydrochloride, 0.2 mg kg-1. intraperitoneal injection of hydroxycodone hydrochloride 30 min after pain caused by formalin The pain behavior of animals was evaluated by cumulative pain score after pain was induced at 1: 10, 20, 30, 45, 6090 min. The rats were anesthetized with chloral hydrate for 90 min after pain. The concentration of NO and substance P were measured by 3 mL blood collection from the heart. According to the results of behavioral changes and pain scores in rats, the model was established successfully. Pain score value: 10 min,C after pain group than B group significantly decreased (P0.05), 60 min,C,D group and B group significantly decreased (P0.05). The concentration of NO in group D was significantly lower than that in group B (P0.01), C and group D, the difference was statistically significant (P0.05). The concentration of substance P in blood in group C and C was significantly lower than that in group B, and the difference was statistically significant (P0. 001), C and group D, respectively) (P0.05). The results showed that the analgesic effect of intraperitoneal injection of hydroxycodone hydrochloride 30 min before and 30 min after pain induced by formalin was correlated with the decrease of NO and substance P in blood. The preemptive analgesic effect of 30min was more significant before pain.
【作者单位】：吉林大学第一医院麻醉科;
【基金】：吉林省卫生厅科研基金资助项目(2008Z017)
【分类号】：S859.791

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