枸杞多糖对己烯雌酚暴露成年仓鼠睾丸Caspase-8、Caspase-9及p53凋亡蛋白的影响
发布时间：2019-02-24 11:39
【摘要】：66只成年雄性仓鼠随机分为空白对照组(A)、DES组(B)、1 mg/kg LBP组(C)、10 mg/kg LBP组(D)、50mg/kg LBP组(E)及VC+VE组(F)。除对照组外,其余5组均皮下注射1mg/kg己烯雌酚,对照组注射等剂量的橄榄油,连续用药7d,同时C、D、E组分别灌服1、10、50mg/kg的LBP,F组灌服100mg/kg的VC及200IU/kg的VE,A、B组灌服等剂量的生理盐水。于末次给药24h后,乙醚麻醉致死仓鼠,取睾丸进行免疫组化检测,确定Caspase-8、Caspase-9和p53的表达。结果显示,通过免疫组化法检测证实,DES引起成年仓鼠睾丸内生精细胞大量凋亡,显著提高Caspase-9的表达(P0.01);给予不同剂量的LBP后,Caspase-9及p53的表达呈剂量依赖性降低,以LBP高剂量组(50mg/kg)表达最低(P0.01),同时LBP高剂量组的Caspase-8阳性表达与对照组相比差异不显著。结果表明,枸杞多糖可以降低己烯雌酚所致成年仓鼠生精细胞中凋亡蛋白Caspase-8、Caspase-9和p53的表达,且以50mg/kg剂量作用最为显著。
[Abstract]:66 adult male hamsters were randomly divided into blank control group, (A), DES group, (B), 1 mg/kg LBP group, (C), 10 mg/kg LBP group, (D), 50mg/kg LBP group (E) and VC VE group (F). In addition to the control group, the other five groups were subcutaneously injected with 1mg/kg diethylstilbestrol. The control group was injected with olive oil of the same dose for 7 days. Meanwhile, the control group was given the VC of 100mg/kg and the VE, of 200IU/kg of 50 mg / kg LBP,F. Group A and B were given the same dose of normal saline. 24 hours after the last administration, the testis were anesthetized to kill hamsters. The expressions of Caspase-8,Caspase-9 and p53 were determined by immunohistochemistry. The results showed that DES induced a large number of apoptosis of spermatogenic cells in the testis of adult hamsters and significantly increased the expression of Caspase-9 (P0.01). After different doses of LBP, the expression of Caspase-9 and p53 decreased in a dose-dependent manner, especially in the high dose LBP group (50mg/kg) (P0.01), and the expression of Caspase-8 in the LBP high dose group was not significantly different from that in the control group. The results showed that Lycium barbarum polysaccharides could decrease the expression of apoptotic protein Caspase-8,Caspase-9 and p53 in adult hamster spermatogenic cells induced by diethylstilbestrol, and 50mg/kg was the most significant one.
【作者单位】： 河北农业大学动物医学院;
【基金】：国家自然科学基金资助项目(31072164) 河北省自然科学基金资助项目(C2010000662)
【分类号】：S859.8
[Abstract]:66 adult male hamsters were randomly divided into blank control group, (A), DES group, (B), 1 mg/kg LBP group, (C), 10 mg/kg LBP group, (D), 50mg/kg LBP group (E) and VC VE group (F). In addition to the control group, the other five groups were subcutaneously injected with 1mg/kg diethylstilbestrol. The control group was injected with olive oil of the same dose for 7 days. Meanwhile, the control group was given the VC of 100mg/kg and the VE, of 200IU/kg of 50 mg / kg LBP,F. Group A and B were given the same dose of normal saline. 24 hours after the last administration, the testis were anesthetized to kill hamsters. The expressions of Caspase-8,Caspase-9 and p53 were determined by immunohistochemistry. The results showed that DES induced a large number of apoptosis of spermatogenic cells in the testis of adult hamsters and significantly increased the expression of Caspase-9 (P0.01). After different doses of LBP, the expression of Caspase-9 and p53 decreased in a dose-dependent manner, especially in the high dose LBP group (50mg/kg) (P0.01), and the expression of Caspase-8 in the LBP high dose group was not significantly different from that in the control group. The results showed that Lycium barbarum polysaccharides could decrease the expression of apoptotic protein Caspase-8,Caspase-9 and p53 in adult hamster spermatogenic cells induced by diethylstilbestrol, and 50mg/kg was the most significant one.
【作者单位】： 河北农业大学动物医学院;
【基金】：国家自然科学基金资助项目(31072164) 河北省自然科学基金资助项目(C2010000662)
【分类号】：S859.8
【参考文献】
相关期刊论文 前7条
1 王志成;赵红光;龚守良;;生精细胞凋亡的线粒体调控[J];吉林大学学报(医学版);2007年01期
2 刘光伟,龚守良;电离辐射诱导的细胞G_2期阻滞[J];国外医学(放射医学核医学分册);2002年04期
3 黄晓兰,杨明亮,吴晓e，
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