不同高度丝素蛋白支架体内原位修复兔膝关节骨软骨缺损的研究
发布时间：2019-03-22 07:43
【摘要】：目的:通过研究不同高度丝素蛋白支架体内原位修复兔膝关节骨软骨缺损的修复机制,为临床应用提供研究基础。方法:将11只新西兰大白兔随机分为2组,包括高支架组(LONG,材料平关节面)、低支架组(SHORT,材料低于软骨下骨板)。在双侧膝关节股骨滑车处制造骨软骨缺损(深度3.5~4.0 mm,直径4.0 mm),将2种支架分别植入双侧膝骨软骨缺损处。术后6周和12周时麻醉并处死动物,行大体形态观察、组织学观察及力学测试。结果:经过结构和功能的综合观察评估,低支架组中骨软骨缺损的修复效果优于高支架组。低支架所修复的关节面更平整,软骨下骨板生长更厚实且力学性能更优越。结论:低于软骨下骨板的丝素蛋白支架能较好地促进关节骨软骨缺损的修复,一定程度上提示着骨软骨支架新的应用方式。
[Abstract]:Aim: to study the mechanism of in situ repair of rabbit knee osteochondral defects with silk fibroin scaffolds in order to provide a basis for clinical application. Methods: eleven New Zealand white rabbits were randomly divided into two groups, including elevated stent group (LONG, material flat articular surface) and low stent group (SHORT, material lower than subchondral bone plate). Osteochondral defects were made at the femoral trochlear of both knee joints (depth 3.5 脳 4.0 mm, diameter 4.0 mm),). Two kinds of scaffolds were implanted into bilateral knee osteochondral defects respectively. The animals were anesthetized and killed at 6 and 12 weeks after operation. Gross morphology observation, histological observation and mechanical test were performed. Results: the repairing effect of osteochondral defect in the low stent group was better than that in the elevated stent group by the comprehensive observation and evaluation of the structure and function. The articular surface repaired by low scaffolds is flatter, the subchondral bone plate grows thicker and the mechanical properties are better. Conclusion: the silk fibroin scaffolds lower than the subchondral plate can promote the repair of articular osteochondral defects, suggesting a new application of osteochondral scaffolds to a certain extent.
【作者单位】： 北京体育大学运动康复系;浙江体育职业技术学院;浙江大学;
【分类号】：R873;G804.2
，
本文编号：2445403
[Abstract]:Aim: to study the mechanism of in situ repair of rabbit knee osteochondral defects with silk fibroin scaffolds in order to provide a basis for clinical application. Methods: eleven New Zealand white rabbits were randomly divided into two groups, including elevated stent group (LONG, material flat articular surface) and low stent group (SHORT, material lower than subchondral bone plate). Osteochondral defects were made at the femoral trochlear of both knee joints (depth 3.5 脳 4.0 mm, diameter 4.0 mm),). Two kinds of scaffolds were implanted into bilateral knee osteochondral defects respectively. The animals were anesthetized and killed at 6 and 12 weeks after operation. Gross morphology observation, histological observation and mechanical test were performed. Results: the repairing effect of osteochondral defect in the low stent group was better than that in the elevated stent group by the comprehensive observation and evaluation of the structure and function. The articular surface repaired by low scaffolds is flatter, the subchondral bone plate grows thicker and the mechanical properties are better. Conclusion: the silk fibroin scaffolds lower than the subchondral plate can promote the repair of articular osteochondral defects, suggesting a new application of osteochondral scaffolds to a certain extent.
【作者单位】： 北京体育大学运动康复系;浙江体育职业技术学院;浙江大学;
【分类号】：R873;G804.2
，
本文编号：2445403
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