异氟醚对新生大鼠脑胶质纤维酸性蛋白和兴奋性氨基酸转运体2表达的影响

发布时间：2019-04-25 22:03

【摘要】：目的研究长时间亚麻醉浓度异氟醚暴露对新生大鼠脑星形胶质细胞胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和兴奋性氨基酸转运体2(excitatory amino acid transporter2,EAAT2)表达的影响。方法出生后7 d的Wistar大鼠40只,采用随机数字表法随机分为两组(n=20),异氟醚组(Iso)和对照组(Con),分别暴露于1.1%异氟醚(0.5 MAC)和30%氧气与氮气混合气体中维持6 h。在处理后12、24 h、3和7 d时,两组均分别取乳鼠新鲜脑组织(n=5),冰冻组织切片后,用GFAP抗体和EAAT2抗体进行免疫荧光双标染色,测得额叶皮质区和海马区的星形胶质细胞GFAP、GFAP和EAAT2共染区的荧光强度。结果相比Con组额叶皮质区,Iso组GFAP的荧光强度在处理后12、24 h显著降低(P0.01),而处理后3、7 d两组差异无统计学意义;海马区比较,Iso组GFAP的荧光强度在处理后12、24 h、3 d相比Con组显著下降(P0.01),处理后7 d差异无统计学意义。Iso组额叶皮质区和海马区GFAP和EAAT2共染区域的荧光强度在处理后12、24 h、3和7 d均显著降低,与Con组差异有统计学意义(P0.01)。结论 1.1%异氟醚暴露会暂时性减少新生大鼠额叶皮质区和海马区GFAP表达,延迟星形胶质细胞的骨架发育;而对星形胶质细胞EAAT2表达抑制是持续性的,这可能是异氟醚对新生大鼠产生脑毒性的机制之一。
[Abstract]:Aim to study the effects of isoflurane on the expression of glial fibrillary acidic protein (glial fibrillary acidic protein,GFAP) and excitatory amino acid transporter 2 (2-(excitatory amino acid transporter2,EAAT2) in astrocytes of neonatal rats exposed to isoflurane for a long time. Methods 40 Wistar rats were randomly divided into two groups (n = 20), isoflurane group (Iso) and control group (Con),). They were exposed to 1.1% isoflurane (0.5 MAC) and 30% oxygen-nitrogen mixture for 6 h, respectively. At 12 h, 24 h, 3 d and 7 d after treatment, the fresh brain tissues of neonatal rats were taken respectively. After frozen sections, the GFAP, of astrocytes in frontal cortex and hippocampus were detected by double immunofluorescence staining with GFAP antibody and EAAT2 antibody. Fluorescence intensity of GFAP and EAAT2 co-staining region. Results compared with the frontal cortex of Con group, the fluorescence intensity of GFAP in Iso group decreased significantly at 12 h and 24 h after treatment (P0.01), but there was no significant difference between the two groups at 3 d and 7 d after treatment. Compared with Con group, the fluorescence intensity of GFAP in Iso group was significantly lower than that in Con group at 12 h, 24 h, and 3 d after treatment (P0.01). The fluorescence intensity of GFAP and EAAT2 co-stained area in frontal cortex and hippocampus in Iso group was significantly lower than that in Con group at 12 h, 24 h, 3 d and 7 d after treatment, and there was significant difference between Iso group and Iso group (P0.01). 3 days and 7 days after treatment, the fluorescence intensity of Iso group was significantly lower than that of Con group (P0.01). Conclusion 1. 1% isoflurane exposure can temporarily reduce the expression of GFAP in frontal cortex and hippocampus and delay the development of cytoskeleton of astrocytes in neonatal rats. The inhibition of EAAT2 expression in astrocytes is persistent, which may be one of the mechanisms of brain toxicity induced by isoflurane in neonatal rats.
【作者单位】：广州医科大学附属第三医院麻醉科;广东省佛山市禅城区中心医院麻醉科;广东省中医院麻醉科;
【基金】：广东省自然科学基金资助项目(S2011010004786)
【分类号】：R722.1

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