丰富环境对七氟醚吸入诱发新生大鼠远期认知功能障碍的影响

发布时间：2019-05-13 13:56

【摘要】：目的观察丰富环境对七氟醚吸入诱发新生大鼠远期认知功能障碍的影响。方法 6日龄新生雄性SD大鼠30只随机均分为三组:O2+标准环境组(OS组)、七氟醚麻醉+标准环境组(SS组)和七氟醚麻醉+丰富环境组(SE组)。SS、SE组分别在日龄6、7、8d时,每天接受1次2h的3%七氟醚麻醉,氧浓度100%;O2组在相应日龄吸入100%氧气。出生后第8天起至行为学测试前分别进行标准环境和丰富环境饲养,连续4周。第35天行旷场实验。第36天行场景性、条件性恐惧实验训练。第37、38天行场景性、条件性恐惧实验测试。行为学测试结束后2h,每组各取4只大鼠海马组织,采用Western blot法检测Synapsin-1、PSD-95、c-fos及BDNF含量。结果旷场实验测试中,三组探索路程和中央格停留时间差异无统计学意义。场景性恐惧实验测试中,与SS组比较,OS、SE组僵直反应明显增加(P0.05)。条件性恐惧实验测试中,三组僵直反应差异无统计学意义。与SS组比较,OS、SE组海马Synapsin-1、PSD-95、c-fos和BDNF含量明显增加(P0.05)。结论七氟醚麻醉可致新生大鼠远期海马依赖性认知功能受损,丰富环境可改善此种损伤,其机制可能与增加突触可塑性相关蛋白表达有关。
[Abstract]:Objective to observe the effect of rich environment on long-term cognitive impairment induced by sevoflurane inhalation in neonatal rats. Methods 30 6-day-old male SD rats were randomly divided into three groups: O2 standard environment group (OS group), sevoflurane anesthesia standard environment group (SS group) and sevoflurane anesthesia rich environment group (SE group). SS and SE groups were at 6 and 7 days of age, respectively. For 8 days, he was anesthetized with 3% sevoflurane once a day for 2 hours with an oxygen concentration of 100%. The O2 group inhaled 100% oxygen at the corresponding age. From the 8th day after birth to the behavioral test, the standard environment and rich environment were raised for 4 weeks. The open field experiment was carried out on the 35th day. On the 36th day, the scene and conditional fear experiment was trained. On the 37th and 38th day, the scene and conditional fear were tested. At 2 hours after behavioral test, 4 rats in each group were taken from hippocampus, and the contents of Synapsin-1,PSD-95,c-fos and BDNF were detected by Western blot method. Results there was no significant difference between the three groups in the distance of exploration and the residence time of the central lattice in the open field test. In the scene fear test, compared with the SS group, the rigid response in the OS,SE group was significantly increased (P 0.05). There was no significant difference in rigid response among the three groups in conditional fear experiment. Compared with SS group, the contents of Synapsin-1,PSD-95,c-fos and BDNF in hippocampus of OS,SE group were significantly increased (P 0.05). Conclusion sevoflurane anesthesia can damage the long-term cognitive function of hippocampal dependence in neonatal rats, and rich environment can improve the damage. The mechanism may be related to the increase of synaptic plasticity related protein expression.
【作者单位】：南京大学医学院临床学院南京军区南京总医院麻醉科;徐州医学院江苏省麻醉学重点实验室;
【基金】：国家自然科学基金(81271216,81300946,81471105)
【分类号】：R614

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