丙泊酚对高脂血症大鼠空间学习记忆功能的影响及机制
发布时间：2019-05-13 14:49
【摘要】：目的:研究丙泊酚对高脂血症大鼠空间学习记忆功能和海马神经元Tau蛋白磷酸化表达水平及老年斑沉积的影响。方法:将成模大鼠随机分成4组:P10、P30、P75组分别腹腔注射丙泊酚(10,30,75mg/kg·d-1),对照组(G组)给予生理盐水75mg/kg·d-1,连续注射5d。Morris水迷宫测试大鼠空间学习记忆功能。取海马组织行刚果红和免疫组织化学染色,ipwin32软件分析Tau蛋白磷酸化表达阳性细胞平均光密度值。结果:定位航行实验及附加实验:P30、P75组逃避潜伏期较P10、G组明显延长(P0.05);空间搜索实验:P30、P75组穿越平台次数较P10、G组明显减少(P0.05);免疫组织化学染色:P30、P75组平均光密度值与P10、G组比较,差异有统计学意义(P0.05)。刚果红染色:P30、P75组可见老年斑沉积物。结论:镇静或麻醉剂量丙泊酚可损害高脂血症大鼠空间学习记忆能力。其机制可能与海马老年斑沉积增多和Tau蛋白过度磷酸化相关。
[Abstract]:Aim: to study the effects of propofol on spatial learning and memory function, phosphorylation of Tau protein in hippocampal neurons and plaque deposition in hyperlipidemia rats. Methods: the model rats were randomly divided into four groups: group P10, group P30, group P75 was injected with propofol (10, 30, 75 mg / kg 路d-1), and the control group (group G) was treated with saline 75mg/ kg 路d-1. The spatial learning and memory function of rats was measured by continuous injection of 5d.Morris water maze. The hippocampal tissues were stained with Congo red and immunohistochemistry, and the average optical density of Tau protein phosphorylation positive cells was analyzed by ipwin32 software. Results: the escape latency of P75 group was significantly longer than that of P10, and the escape latency of P75 group was significantly longer than that of P10 group (P 0.05), and the number of platform crossing in P75 group was significantly lower than that of P10 group (P 0.05), and the escape latency of P75 group was significantly longer than that of P10 group (P 0.05). The average optical density of P30 and P75 groups was significantly different from that of P10 and G groups (P 0.05). Congo red staining: P30, P75 group can see the deposit of old age spot. Conclusion: sedation or anaesthesia dose propofol can damage the spatial learning and memory ability of hyperlipidemia rats. The mechanism may be related to the increase of hippocampal plaque deposition and hyperphosphorylation of Tau protein.
【作者单位】： 广西桂林医学院;北京师范大学心理学院;中国人民解放军第303医院;广西医科大学第一附属医院呼吸内科;
【基金】：广西自然科学基金资助项目(No.桂科自0728253)
【分类号】：R614
本文编号：2475974
[Abstract]:Aim: to study the effects of propofol on spatial learning and memory function, phosphorylation of Tau protein in hippocampal neurons and plaque deposition in hyperlipidemia rats. Methods: the model rats were randomly divided into four groups: group P10, group P30, group P75 was injected with propofol (10, 30, 75 mg / kg 路d-1), and the control group (group G) was treated with saline 75mg/ kg 路d-1. The spatial learning and memory function of rats was measured by continuous injection of 5d.Morris water maze. The hippocampal tissues were stained with Congo red and immunohistochemistry, and the average optical density of Tau protein phosphorylation positive cells was analyzed by ipwin32 software. Results: the escape latency of P75 group was significantly longer than that of P10, and the escape latency of P75 group was significantly longer than that of P10 group (P 0.05), and the number of platform crossing in P75 group was significantly lower than that of P10 group (P 0.05), and the escape latency of P75 group was significantly longer than that of P10 group (P 0.05). The average optical density of P30 and P75 groups was significantly different from that of P10 and G groups (P 0.05). Congo red staining: P30, P75 group can see the deposit of old age spot. Conclusion: sedation or anaesthesia dose propofol can damage the spatial learning and memory ability of hyperlipidemia rats. The mechanism may be related to the increase of hippocampal plaque deposition and hyperphosphorylation of Tau protein.
【作者单位】： 广西桂林医学院;北京师范大学心理学院;中国人民解放军第303医院;广西医科大学第一附属医院呼吸内科;
【基金】：广西自然科学基金资助项目(No.桂科自0728253)
【分类号】：R614
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