星状神经节阻滞对SD大鼠离体心功能的影响
发布时间：2019-06-18 23:42
【摘要】：目的：SD大鼠行星状神经节阻滞，观察星状神经节阻滞对Langendorff离体心脏灌流装置离体心功能的影响，为星状神经节阻滞用于临床改善心功能减少围手术期心血管事件发生提供理论依据。 方法：将24只8～10周龄体重180～220克的雄性SD大鼠采用随机数字表法随机分为3组，每组8只：(1)左侧星状神经节阻滞组(LS组)；(2)右侧星状神经节阻滞组(RS组)；(3)手术对照组（C组）。大鼠腹腔内注射完成麻醉后，钝性分开左右胸头肌，沿食管旁找到星状神经节，埋管并缝皮，7天后行星状神经节阻滞实验，观察是否出现霍纳综合征，并连续给药两周。星状神经节阻滞模型建立成功后，应用Langendorff离体心脏灌流装置建立SD大鼠离体心脏灌流模型。大鼠腹腔注射肝素后，仰卧位固定，迅速开胸，取出心脏，置于4℃冷的K-H液中分离主动脉，悬挂于Langendorff离体心脏灌流装置上。K-H液灌注15～20分钟，达到平衡末时测定冠脉流量（CF），记录相关的心功能指标：心率（HR）左室发展压（LVDP）左室舒张末压（LVEDP）收缩期心室内压力升高速率（+dp/dt）舒张期心室内压力升高速率（-dp/dt）。同时取下心脏离心后取上清，经过加标准品加样加抗体洗涤以及加底物等步骤后，用ELISA法检测心肌组织中肾上腺素（EPI）去甲肾上腺素（NE）神经肽Y（NPY）和血管活性肠肽（VIP）的浓度。 结果：1.与C组相比，RSGB组LVDP+dp/dtmax-dp/dtmaxCF及HR值均升高有统计学意义(P＜0.05或0.01)，RSGB组的LVEDP值降低有统计学意义（P＜0.01）；与C组相比，LSGB组LVDP+dp/dtmax-dp/dtmax及CF值均升高有统计学意义(P＜0.05或0.01)，LSGB组的LVEDP值降低有统计学意义（P＜0.05）。与LSGB组相比，RSGB组LVDP+dp/dtmax及-dp/dtmax值升高有统计学意义（P＜0.05），RSGB组LVEDP值降低有统计学意义（P＜0.05）。2.与C组相比，RSGB组和LSGB组EPINENPY及VIP值均升高有统计学意义(P＜0.05或0.01)。与LSGB组相比，RSGB组EPINE及NPY值均升高有统计学意义（P＜0.05）。结论：1.星状神经节阻滞通过增强心肌的收缩和舒张功能，对SD大鼠离体心功能有促进作用；2.左右两侧星状神经节对心脏的交感神经支配区域不同，，两侧星状神经节阻滞对大鼠离体心功能的影响存在差异，右侧星状神经节阻滞增强心功能的效果更佳。
[Abstract]:Objective: To observe the effect of stellate ganglion block on the isolated cardiac function of the isolated cardiac perfusion device in the rat of SD rat. So as to provide a theoretical basis for reducing the occurrence of cardiovascular events in the perioperative period. Method:24 to 10 weeks Age? Male SD rats weighing 180 ~ 220 g were randomly divided into 3 groups by random digital method.8 of each group: (1) left stellate ganglion block (LS group); (2) right stellate ganglion block (RS group); (3) operation control group (group C (1) After the intraperitoneal injection of the rat was completed, the left and right pectoral muscles were separated, and the stellate ganglion, the buried tube and the skin were found along the esophagus. After 7 days, the planetary ganglion block was used to observe the presence of the Horner's syndrome. Week. After the establishment of the stellate ganglion block model, the isolated cardiac perfusion model of SD rats was established by the Langendorff in-vitro cardiac perfusion device. Type: After the rats were injected with heparin, the rats were fixed in the supine position, the chest was quickly opened, the heart was removed, the aorta was isolated from the K-H solution cold at 4 鈩
本文编号：2501886
[Abstract]:Objective: To observe the effect of stellate ganglion block on the isolated cardiac function of the isolated cardiac perfusion device in the rat of SD rat. So as to provide a theoretical basis for reducing the occurrence of cardiovascular events in the perioperative period. Method:24 to 10 weeks Age? Male SD rats weighing 180 ~ 220 g were randomly divided into 3 groups by random digital method.8 of each group: (1) left stellate ganglion block (LS group); (2) right stellate ganglion block (RS group); (3) operation control group (group C (1) After the intraperitoneal injection of the rat was completed, the left and right pectoral muscles were separated, and the stellate ganglion, the buried tube and the skin were found along the esophagus. After 7 days, the planetary ganglion block was used to observe the presence of the Horner's syndrome. Week. After the establishment of the stellate ganglion block model, the isolated cardiac perfusion model of SD rats was established by the Langendorff in-vitro cardiac perfusion device. Type: After the rats were injected with heparin, the rats were fixed in the supine position, the chest was quickly opened, the heart was removed, the aorta was isolated from the K-H solution cold at 4 鈩
本文编号：2501886
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