大黄灵仙颗粒调控IL-6、EGR-1表达治疗胆石症的实验研究

发布时间：2019-06-19 12:58

【摘要】：目的:明确大黄灵仙颗粒对慢性肝损伤胆石病兔模型的疗效,研究中药复方对关键致石因素IL-6、EGR-1表达的调控作用。方法:(1)将50只雄性新西兰大白兔随机分为3组:正常对照组、肝纤维化胆石病组、肝硬化胆石病组,其中正常对照组10只,其余两组各20只,在金博的慢性肝损伤兔模型构建方法的基础上,同时予高热量、高胆固醇致石饲料,建立慢性肝损伤胆石病兔模型。(2)造模12周后,病理明确诊断肝损伤形成后,将各组剩余实验兔分别分为对照组、治疗组,其中治疗组每天灌胃中药制剂大黄灵仙颗粒0.4 g/(kg·d),其余各组灌胃等量生理盐水,各组大白兔均正常饲料饲养。用药第13周,麻醉后取肝脏组织,分别使用实时荧光定量PCR、免疫组化等方法进行检测。结果:(1)常规病理:与对照组相比,用药各组光镜下可见遭到破坏的肝小叶逐渐修复,部分肝细胞形态恢复正常,炎性细胞及纤维组织减少,区域呈点状、片状坏死明显减少;(2)免疫组化:与对照组相比,喂食大黄灵仙颗粒剂的各组肝窦、汇管区的肝细胞胞浆或胞膜上棕褐色颗粒沉积表达明显减少,小胆管胆汁淤积减少,说明用药后肝损伤得到改善;(3)q PCR检测结果:与对照各组相比,用药各组的IL-6、EGR-1 mRNA的表达量均有不同程度降低(P0.05),说明该药可有效减轻肝损伤。结论:本研究结果表明大黄灵仙颗粒对慢性肝损伤性胆石病动物模型具有较明显的改善和防治作用。
[Abstract]:Objective: to investigate the efficacy of rhubarb Lingxian granule in the treatment of cholelithiasis rabbit model of chronic liver injury and to study the regulatory effect of traditional Chinese medicine (TCM) on the expression of IL-6,EGR-1, a key lithogenic factor. Methods: (1) Fifty male New Zealand white rabbits were randomly divided into three groups: normal control group, hepatic fibrosis cholelithiasis group and cirrhotic cholelithiasis group, including normal control group (n = 10) and other two groups (n = 20). On the basis of Jinbo's model of chronic liver injury, rabbits with chronic liver injury were given high calorie and high cholesterol diet at the same time to establish chronic liver injury cholelithiasis rabbit model. (2) after 12 weeks, After pathological diagnosis of liver injury, the remaining experimental rabbits in each group were divided into control group and treatment group, in which the treatment group was fed with 0.4 g / (kg 路d),) of rhubarb Lingxian granule every day, and the rabbits in each group were fed with normal diet. At the 13th week of administration, the liver tissues were taken after anesthesia and detected by real-time fluorescence quantitative PCR, (real-time fluorescence quantitative PCR). Results: (1) routine pathology: compared with the control group, the damaged hepatic lobules were gradually repaired under light microscope, the morphology of some hepatocytes returned to normal, inflammatory cells and fiber tissue decreased, the area was punctate and flake necrosis was obviously decreased. (2) Immunochemistry: compared with the control group, the expression of brown particles in the cytoplasm or membrane of hepatocytes and cholestasis of small bile duct in each group fed with rhubarb Lingxian granule was significantly decreased, which indicated that the liver injury was improved after administration of rhubarb Lingxian granule. (3) the results of) q PCR detection: compared with the control group, the expression of IL-6,EGR-1 mRNA in each group decreased in varying degrees (P 0.05), which indicated that the drug could effectively reduce liver injury. Conclusion: rhubarb Lingxian granule can obviously improve and prevent the animal model of cholelithiasis induced by chronic liver injury.
【作者单位】：广西中医药大学第一附属医院;右江民族医学院;广西中医药大学;
【基金】：国家自然科学基金面上项目(81470198) 2014年广西科学研究与技术开发计划项目(桂科能14123006-3)
【分类号】：R285.5

【参考文献】