氯胺酮对未成年大鼠学习记忆与海马Egr-1的影响

发布时间：2019-06-25 13:39

【摘要】：目的：探讨未成年SD大鼠行氯胺酮注射麻醉后对大鼠学习和记忆功能以及海马即刻早期基因Egr-1表达的影响。方法：选择20只雄性SD未成年大鼠（21日龄），随机分为2组，，每组10只，即氯胺酮（Ket）组、生理盐水对照（Ns）组。未成年SD大鼠在实验时间以外可以自由地取食和饮水。Ket组大鼠腹腔注射Ket50mg/kg （Ns稀释至1ml），Ns组则给予等容量的生理盐水，持续6天。在药物注射期间注意观察监测并维持大鼠的生命体征正常。于给药后24小时行Morris水迷宫定位航行实验，测定其学习和记忆能力。于末次Morris水迷宫空间探索实验结束后1h，腹腔注射麻醉剂（10%水合氯醛400mg/kg）后快速断头冰上取脑。采用实时荧光定量PCR法检测未成年SD大鼠海马Egr-1mRNA的表达情况，免疫印迹Western blotting法检测海马Egr-1蛋白的表达。结果：（1）与Ns组比较， Ket组大鼠在定位航行和空间探索实验中隐蔽平台逃避潜伏期显著延长（P 0.05）；活动总路径缩短，穿越平台次数明显减少（P 0.05）。（2）两实验组海马组织Egr-1mRNA均有一定量的表达，但与Ns组相比，Ket组Egr-1mRNA表达量下调，差异具有明显的有统计学意义（P 0.05）。（3）Ket组大鼠海马即刻早期基因Egr-1蛋白表达水平明显低于Ns组（P 0.05）。结论：未成年SD大鼠反复给予亚麻醉剂量的氯胺酮注射后，可降低大鼠在Morris水迷宫中的学习和记忆能力，并且同时也导致未成年SD大鼠海马即刻早期基因Egr-1表达的降低。氯胺酮对未成年SD大鼠海马即刻早期基因Egr-1表达的影响可能是其导致大鼠行为学改变的机制。
[Abstract]:Aim: to investigate the effects of ketamine injection anesthesia on learning and memory function and immediate early gene Egr-1 expression in hippocampus of juvenile SD rats. Methods: twenty male SD underage rats (21 days old) were randomly divided into two groups: ketamine (Ket) group (n = 10) and saline control group (Ns) group). The underage SD rats were free to eat and drink outside the experimental time. The rats in the Ket group were injected intraperitoneally with Ket50mg/kg (Ns diluted to 1ml), Ns group were given the same volume of saline for 6 days). Pay attention to observe, monitor and maintain the vital signs of rats during drug injection. The learning and memory ability of Morris water maze navigation experiment was measured 24 hours after administration. At the end of the last Morris water maze space exploration experiment, the brain was quickly decapitated on ice after intraabdominal injection of anesthetic (10% chloral hydrate 400mg/kg). The expression of Egr-1mRNA in hippocampus of juvenile SD rats was detected by real-time fluorescence quantitative PCR, and the expression of Egr-1 protein in hippocampus was detected by immunoblotting Western blotting. Results: (1) compared with Ns group, the escape latency of hidden platform in Ket group was significantly longer than that in Ket group. The expression of Egr-1mRNA in hippocampal tissue of both groups was significantly lower than that of Ns group, but the expression of Egr-1mRNA in Ket group was significantly lower than that in Ns group (P 0.05). (3). The expression of Egr-1 protein in hippocampus of Ket group was significantly lower than that of Ns group (P 0.05). Conclusion: repeated injection of ketamine into juvenile SD rats can decrease the learning and memory ability of rats in Morris water maze, and also lead to the decrease of immediate early gene Egr-1 expression in hippocampus of juvenile SD rats. The effect of ketamine on the expression of immediate early gene Egr-1 in hippocampus of juvenile SD rats may be the mechanism of behavioral changes in rats.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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