TGF-β介导盆腔淋巴管放射性纤维化作用机制的研究

发布时间：2019-07-19 15:46

【摘要】：目的:探讨转化生长因子-β(TGF-β)介导盆腔淋巴管放射性纤维化作用的机制。方法:20只雌性新西兰大白兔随机分成空白对照组(Sham组)、照射后4周组、照射后12周组和照射后16周组。除Sham组外,其余各组大白兔均给予放射线单次、大剂量照射。实验第4周、第12周和第16周末用水合氯醛麻醉各组大白兔,解剖,取盆腔淋巴管,染色光镜下分别观察盆腔淋巴管组织病理变化。提取盆腔淋巴管组织,采用蛋白印迹法检测TGF-β、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)的表达变化。结果:Sham组大白兔盆腔淋巴管组织中细胞排列整齐,无炎性细胞浸润;照射后4周可见少量炎性细胞浸润和水肿;照射后12周可见红细胞和巨噬细胞聚集,大量炎性细胞浸润、水肿显著;照射后16周可见管壁增厚、炎性细胞浸润明显,胶原增生明显。Azan染色Sham组盆腔淋巴管组织中未见纤维化表达,照射后4周大白兔盆腔淋巴管组织中少见蓝色纤维化表达,照射后12周和16周直肠组织中可见大量纤维化表达。盆腔淋巴管组织中TGF-β蛋白表达随照射后时间的延长表达增加,核内HIF-1α蛋白以及细胞浆内VEGF蛋白表达也随照射后时间的延长表达增加。结论:照射增加TGF-β蛋白表达可能与HIF-1α和VEGF蛋白相关,TGF-β可能通过影响HIF-1α从而调控盆腔淋巴管放射性纤维化。
[Abstract]:Objective: to investigate the mechanism of radiation fibrosis of pelvic lymphoid vessels mediated by transforming growth factor-尾 (TGF- 尾). Methods: twenty female New Zealand white rabbits were randomly divided into blank control group (Sham group), 4 weeks after irradiation group, 12 weeks after irradiation and 16 weeks after irradiation. Except Sham group, all the rabbits in the other groups were treated with single radiation and high dose irradiation. At the 4th week, 12th week and 16th weekend, the rabbits in each group were anesthetized with chloraldehyde hydrate. The pelvic lymphatic vessels were dissected and the pathological changes of pelvic lymphoid vessels were observed under staining light microscope. The expression of TGF- 尾, hypoxia inducible factor-1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) were detected by Western imprinting. Results: in Sham group, the cells in pelvic lymphoid tissue of rabbits were neatly arranged and no inflammatory cells infiltrated, a small amount of inflammatory cell infiltration and edema were observed at 4 weeks after irradiation, and red blood cells and macrophages were aggregated at 12 weeks after irradiation, a large number of inflammatory cells infiltrated and edema was significant. At 16 weeks after irradiation, the wall thickened, inflammatory cells infiltrated obviously, collagen proliferation was obvious. Azan staining Sham group showed no fibrosis expression in pelvic lymphoid tissue, blue fibrosis expression was rare in pelvic lymphoid tissue of rabbits 4 weeks after irradiation, and a large number of fibrosis expression was found in rectal tissue 12 weeks and 16 weeks after irradiation. The expression of TGF- 尾 protein in pelvic lymphoid tissue increased with the prolongation of irradiation time, and the expression of HIF-1 伪 protein in nucleus and VEGF protein in cytoplasm also increased with the prolongation of irradiation time. Conclusion: the increase of TGF- 尾 protein expression by irradiation may be related to HIF-1 伪 and VEGF protein. TGF- 尾 may regulate pelvic lymphatic radiation fibrosis by affecting HIF-1 伪.
【作者单位】：新疆医科大学第五附属医院;
【基金】：国家自然科学基金(编号:81260355)
【分类号】：R737.3

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