NMDA受体在小鼠小脑皮层浦肯野细胞自发性活动中的作用机制

发布时间：2021-03-06 21:39

　　[目的]N-甲基-D-天冬氨酸受体是离子型谷氨酸受体,它参与神经元突触后反应以及调控突触前神经递质的释放。在中枢神经系统中,它对突触传递的调控起着关键作用。众所周知,NMDA受体在哺乳动物的小脑皮层、颗粒细胞膜、平行纤维以及分子层中间神经元上广泛表达。在离体条件下,NMDA受体的活化增强浦肯野细胞的抑制性突触后电流。然而,在在体条件下,NMDA对小脑神经元的活动影响还不清楚。因此,在我们的实验中,应用电生理膜片钳记录和药理学方法,探索外源性NMDA对乌拉坦麻醉小鼠小脑皮层浦肯野细胞自发性活动的影响,了解NMDA对小鼠小脑皮层浦肯野细胞自发性简单峰电位放电的影响机制。[方法]实验选取25只6-8周龄成年ICR小鼠,并对其进行腹腔注射乌拉坦麻醉（1.3 g/kg）。待40分钟麻醉结束后,小鼠行气管插管术,使其呼吸道保持通畅,并将小鼠固定在脑立体定位装置上。在小脑部位制作一个圆形的灌流槽,对小鼠小脑蚓部行开颅手术,打孔直径约为1-1.5 mm,暴露小脑表面的记录区域,并轻轻地揭掉硬脑膜,使用蠕动泵在小脑表面持续不断地灌流（0.4 ml/min）含95%02和5% CO2的人工脑脊液。用体温维... 

【文章来源】：延边大学吉林省 211工程院校

【文章页数】：88 页

【学位级别】：博士

【文章目录】：
ABBREVIATION
Chapter 1 Effect of NMD A on spontaneous simple spikes of cerebellar Purkinje cellsin vivo in mice
    Abstract
    摘要
    1.1 Introduction
    1.2 Materials and Methods
        1.2.1 Animals and Anesthesia
        1.2.2 Experiment Equipment
        1.2.3 Surgical procedures
        1.2.4 Electrophysiological recordings
        1.2.5 Drug administration
        1.2.6 Statistical analysis
    1.3 Results
        1.3.1 Cerebellar surface application of NMDA decreased SS firing of PCs
A receptor">        1.3.2 NMDA reduced PC SS firing via the activation of GABA_A receptor
        1.3.3 Cerebellar surface application of NMDA evoked the excitation of MLIs
    1.4 Discussion
        1.4.1 NMDA onduced inhibition rather than excitation of cerebellar PCs
        1.4.2 NMDA mediated the excitation of cerebellar MLIs
        1.4.3 MLIs play a key rolr in controlling the output of PCs
    1.5 Conclusion
    References
Chapter 2 Effect of NMDA on spontaneous complex spikes of cerebellar Purkinjecells in vivo in mice
    Abstract
    摘要
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Animals and Anesthesia
        2.2.2 Experiment Equipment
        2.2.3 Surgical procedures
        2.2.4 Electrophysiological recordings
        2.2.5 Drug administration
        2.2.6 Statistical analysis
    2.3 Results
        2.3.1 Effects of NMDA on CS activity of PCs in the cerebellar cortex
        2.3.2 NMDA enhanced CS-evoked AHP currents
        2.3.3 Blockade of NMDAR attenuated the strength of spontaneous CS
        2.3.4 Blockade AMPARs abolished spontaneous CS firing and NMDARs activity
    2.4 Discussion
    2.5 Conclusions
    References
Review NMDA receptor and it's funcation in cerebellar cortex
    References
Publication
Acknowledgement



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