丙泊酚对小鼠小脑皮层分子层和颗粒细胞层感觉信息传递的影响

发布时间：2021-04-01 14:04

　　[目的]丙泊酚是一种中枢神经系统抑制剂,在体内条件下剂量依赖性的抑制大脑新皮层的信息处理。然而,丙泊酚对活体动物小脑皮质分子层感觉信息处理的影响还不清楚。因此,我们在乌拉坦麻醉麻醉下,应用电生理学和药理学方法研究丙泊酚对感觉刺激诱发小鼠小脑分子层场电位反应的影响。[方法]6-8周龄的成年ICR小鼠通过腹腔注射体重为1.3g/kg乌拉坦麻醉后,进行气管插管以避免呼吸道堵塞,然后将小鼠固定在定制的立体定位装置上。在小脑相应部位作一个防水外流灌流槽后,在小脑Crus Ⅱ区的相对应部位行开颅手术,钻一个直径为1-1.5mm的小孔,暴露记录部位的小脑表面,并用蠕动泵在暴露脑表面持续灌流含氧的人工脑脊液（artificial cerebrospinal fluid, ACSF）o使用Axopatch-200B膜片钳放大器（Molecular Devices, Foster City, CA）记录感觉刺激诱发小脑皮层分子层场电位,并通过D/A转换器1440和Clampex 10.3软件获取感觉刺激诱发场电位活动的数据。记录电极内填充的是人工脑脊液,阻抗在3-5 MΩ之间。丙泊酚和gabazine购置... 

【文章来源】：延边大学吉林省 211工程院校

【文章页数】：89 页

【学位级别】：博士

【文章目录】：
英文缩写词表
Chapter 1 Propofol modulates sensory information processing in cerebellar molecular layerin vivo in mice
    Abstract
    摘要
    1.1 Introduction
    1.2 Experimental procedure
        1.2.1 Anesthesia and surgical procedures
        1.2.2 Electrophysiological recordings and drug application
        1.2.3 Statistical analysis
    1.3 Results
        1.3.1 Effects of propofol on the facial stimulation-evoked field potential responsesin cerebellar molecular layer
A receptoractivity">        1.3.2 Propofol affected sensory responses via the modulation of GABA_A receptoractivity
    1.4 Discussion
        1.4.1 Propofol inhibited the sensory stimulation evokes GABAergic component offield potential responses in cerebellar molecular layer
        1.4.2 Propofol enhanced the sensory stimulation evokes excitatory component offield potential responses in cerebellar molecular layer
A receptor">        1.4.3 Propofol modulated the sensory stimulation-evoked field potentialresponses in cerebellar molecular layer via GABA_A receptor
    1.5 Conclusion
    Acknowledgement
    References
Chapter 2 Propofol enhances facial stimulation-evoked responses in
    Abstract
    摘要
    2.1 Introduction
    2.2 Experimental procedure
        2.2.1 Anesthesia and surgical procedures
        2.2.2 Electrophysiological recordings and drug application
        2.2.3 Chemicals
        2.2.4 Statistical analysis
    2.3 Results
        2.3.1 Effects of propofol on the facial stimulation-evoked field potential responsesin cerebellar granule cell layer
on in cerebellar granule cell layer">        2.3.2 Blocking GABAa receptor activity failed to prevent the propofol enhancedfacial stimulation-evoked R_on in cerebellar granule cell layer
on, and abolished the propofol-induced enhancement of R_on">        2.3.3 Blockade of NMDA receptors activity attenuated facial stimulation-evokedR_on, and abolished the propofol-induced enhancement of Ron

    2.4 Discussion
        2.4.1 P roperties of sensory information transferred by cerebellar granule cell
        2.4.2 Propofol affects sensory information transferring in cerebellar granule
A receptor">        2.4.3 Propofol enhanced facial stimulation evoked responses in granule cell layervia modulation of NMDA receptor, but not GABA_A receptor
    2.5 Conclusions
    Acknowledgement
    References
Review:Mechanisms of propofol modulate neuronal activity and synaptictransmission
    References
在读博士期间申请到的课題、获得的奖项及发表的论文
致谢



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