肾积水后缺氧诱导因子-1、血管内皮生长因子、微小RNA-210的动态表达

发布时间：2017-12-26 21:40

本文关键词：肾积水后缺氧诱导因子-1、血管内皮生长因子、微小RNA-210的动态表达　出处：《重庆医学》2015年30期 　论文类型：期刊论文

【摘要】：目的观察小鼠肾积水后缺氧诱导因子-1(HIF-1)、血管内皮生长因子(VEGF)、微小RNA(miRNA)-210在不同时间点的表达变化并探讨其调控机制。方法采用丝线结扎单侧输尿管的方法制备急性完全性单侧肾积水模型(UUO模型),分为假手术对照组和单侧肾积水组,于单侧肾积水后2、5、9、14d分别颈椎脱臼处死。采用实时荧光定量PCR检测肾积水后患肾各时间点HIF-1mRNA、VEGF mRNA及miRNA-210的表达变化,采用Western blot方法检测肾积水后各时间点HIF-1蛋白的表达变化。结果 HIF-1mRNA表达水平在肾积水后表达量逐渐上调(P0.05)。VEGF mRNA及miRNA-210表达水平在肾积水后2d升高并达到高峰,于UUO后5、9、14d表达量逐渐下调(P0.05)。HIF-1蛋白表达量逐渐上调。结论肾积水后HIF-1mRNA及蛋白表达逐步上调,VEGF mRNA及miRNA-210表达一过性上调,可能与肾积水后肾皮质被压迫机体对缺氧、缺血产生适应性反应有关。
[Abstract]:Objective To observe the expression changes of hypoxia inducible factor -1 (HIF-1), vascular endothelial growth factor (VEGF) and tiny RNA (miRNA) -210 at different time points after hydronephrosis in mice, and to explore their regulatory mechanisms. Methods a rat model of acute complete unilateral hydronephrosis (UUO model) was prepared by silk ligation of unilateral ureter. It was divided into sham operation control group and unilateral hydronephrosis group. Cervical dislocations were executed at 2, 5, 9 and 14d after unilateral hydronephrosis. Real time fluorescence quantitative PCR was used to detect the expression changes of HIF-1mRNA, VEGF mRNA and miRNA-210 at different time points after hydronephrosis. Western blot was used to detect the expression of HIF-1 protein at different time points after hydronephrosis. Results the expression of HIF-1mRNA expression increased gradually after hydronephrosis (P0.05). The expression level of VEGF mRNA and miRNA-210 increased and reached the peak of 2D after hydronephrosis, and the expression of 5, 9 and 14d decreased gradually after UUO (P0.05). The expression of HIF-1 protein was gradually up-regulated. Conclusion the expression of HIF-1mRNA and protein is gradually up-regulated after hydronephrosis, and the expression of VEGF mRNA and miRNA-210 is up regulated. It may be related to the adaptive response of renal cortex compressed organism to hypoxia and ischemia after hydronephrosis.
【作者单位】：南昌大学第一附属医院泌尿外科;
【基金】：江西省科技厅支撑计划(20111BBG70025-1) 江西省自然科学基金(2010GZY0314)
【分类号】：R692.2
【正文快照】： 微小RNA(miRNA)是在真核生物中发现的一类内源性的具有调控功能的非编码RNA,其能抑制或降低靶基因mR-NA的翻译或使其表达量下调[1]。miRNA-210是一种具有缺氧诱导活性的miRNA,在缺氧条件下表达可上调[2]。缺氧诱导因子-1(HIF-1)是一随着细胞内氧浓度的变化而调节靶基因表达的

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