大鼠衰老过程中勃起功能改变与去乙酰化酶SIRT1的关系研究

发布时间：2017-12-28 18:02

本文关键词：大鼠衰老过程中勃起功能改变与去乙酰化酶SIRT1的关系研究　出处：《中华男科学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的:研究衰老过程中大鼠勃起功能变化、抗衰老蛋白SIRT1表达及其相关因子改变,探索SIRT1与老年性勃起功能障碍(ED)发生的关系。方法:SD大鼠按不同月龄分组,利用电刺激大鼠盆腔星状神经节法测定阴茎海绵体内压(ICP),颈动脉穿刺测定平均动脉压(MAP),ICP/MAP作为勃起功能的评价指标;马松染色观察胶原纤维变化;Western印迹测定不同月龄大鼠阴茎海绵体中SIRT1、P53及FOXO3a的表达变化;NO、c GMP检测试剂盒测定阴茎海绵体中NO、c GMP含量。结果:随月龄增长,大鼠勃起功能(ICP/MAP)在8月龄达到峰值,其后随月龄增长降低。c GMP含量变化与勃起功能一致。随月龄增加,大鼠阴茎海绵体中胶原纤维增多。SIRT1的表达随月龄增加不断降低;而凋亡因子P53、氧化应激因子FOXO3a的表达随月龄增加不断上升。结论:NO/c GMP通路活性降低、凋亡及氧化应激可能是衰老导致ED的原因。抗衰老蛋白SIRT1与其调控的凋亡、氧化应激因子改变与勃起功能一致,推测SIRT1与老年性ED的发病有关。
[Abstract]:Objective: To investigate the changes of erectile function, the expression of anti-aging protein SIRT1 and related factors in aging rats, and to explore the relationship between SIRT1 and the pathogenesis of erectile dysfunction (ED). Methods: SD rats were divided into two groups according to different age, determination of intracavernous pressure by electrical stimulation of pelvic stellate ganglion in rats (ICP), carotid artery puncture determination of mean arterial pressure (MAP, ICP/MAP) as the evaluation index of erectile function; to observe the changes of collagen fiber Masson staining; Western blot to determine the expression changes of SIRT1 and P53 FOXO3a and penis of rats of different ages in NO, C; GMP detection kit for determination of NO and C in corpus cavernosum GMP content. Results: the erectile function (ICP/MAP) reached a peak at 8 month old with the increase of the age of the month, and then decreased with the age of the month. The changes of C GMP content were in accordance with the erectile function. With the increase of age, the collagen fibers in the corpus cavernosum of the rat were increased. The expression of SIRT1 decreased with the increase of monthly age, while the expression of apoptotic factor P53 and oxidative stress factor FOXO3a increased with the increase of monthly age. Conclusion: the decrease in activity of NO/c GMP pathway, apoptosis and oxidative stress may be the cause of ED in aging. Anti senescence protein SIRT1 is associated with its regulation of apoptosis, oxidative stress factor changes and erectile function, and that SIRT1 is associated with the onset of senile ED.
【作者单位】：南京大学医学院附属鼓楼医院男科;
【分类号】：R698+.1
【正文快照】： Supported by a grant from National Natural Science Foundation of China(80772175).Correspondence to:DAI Yu-tian,email:ytdai@hotmail.comReceived:September 6,2016;accepted:November 8,2016在勃起功能障碍(erectile dysfunction,ED)的病因中,年龄被认为是一重要的高

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