促红细胞生成素对糖尿病大鼠造影剂急性肾损伤的影响

发布时间：2018-01-09 23:13

本文关键词：促红细胞生成素对糖尿病大鼠造影剂急性肾损伤的影响　出处：《现代预防医学》2015年02期 　论文类型：期刊论文

【摘要】：目的探讨促红细胞生成素(EPO)对糖尿病(DM)鼠造影剂急性肾损伤(CI-AKI)的保护作用及其机制。方法 SD实验大鼠28只,随机分为正常组(CON组,n=7),糖尿病组(DM组,n=7)、造影剂组(CM组,n=7)、EPO预处理组(EPO组,n=7)。观察造影后各时间点6 h、12 h、24 h、48 h、72 h血肌酐(Scr)、尿肾损伤因子(Kim-1)的变化。并检测肾皮质内丙二醛(MDA)含量、超氧化物歧化酶(SOD)的活性变化。结果与DM组相比,CM组第48 h肾皮质MDA含量升高,SOD活性下降,而EPO组肾皮质MDA含量下降,SOD活性升高;CM组Scr第6 h、12 h差异无统计学意义(P0.05),24 h、48 h、72 h均显著升高(P0.05),EPO组Scr 24 h、48 h、72 h则显著下降(P0.01);尿Kim-1各时间点CM组较CON组显著增加(P0.05),而EPO组较CM组则显著减少(P0.05)。结论促红细胞生成素预处理能降低急性肾损伤后Scr、Kim-1的水平,抑制氧化应激反应,减轻糖尿病大鼠造影剂所致的急性肾损伤。
[Abstract]:Objective to investigate the protective effect of erythropoietin (EPO) on acute renal injury (CI-AKI) in diabetic rats with DM.Methods 28 SD rats were tested. They were randomly divided into normal group (Con group), diabetic group (DM group), and contrast agent group (CM group). EPO group was pretreated with EPO. The serum creatinine creatinine (Scrs) was observed at 6 h, 12 h, 24 h, 48 h and 72 h after angiography. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in renal cortex were measured. The results were compared with those in DM group. In CM group, the content of MDA in renal cortex increased and the activity of sod decreased at 48h, while in EPO group, the content of MDA in renal cortex decreased and the activity of sod increased. In CM group, there was no significant difference in Scr at the 6th hour and 12th hour (P 0.05, P 0.05 at 24 h and 48 h and 72 h, respectively). In EPO group, Scr decreased significantly at 24 h, 48 h and 72 h (P 0.01). The urinary Kim-1 in CM group was significantly higher than that in CON group (P 0.05). Conclusion Erythropoietin pretreatment can reduce the level of Scr-Kim-1 and inhibit oxidative stress after acute renal injury in EPO group compared with CM group. To alleviate acute renal injury induced by contrast medium in diabetic rats.
【作者单位】：山东省潍坊医学院临床学院(潍坊医学院附属医院)肾内科;
【基金】：潍坊医学院科技创新基金项目(2012 K11QC1010)
【分类号】：R587.2;R692.9
【正文快照】： 随着造影剂在临床医疗中应用的增加,使得造影剂肾病(Contrast-Induced Nephropathy,CIN)日趋增多,成为造影剂常见的主要并发症之一。据报道,普通人群发病率为0.6%~2.3%,而在糖尿病(Diabetes Mellitus,DM)及慢性肾脏病患者中上升至50%[1,2],糖尿病已成为CIN的独立危险因素,CIN

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