膀胱癌microRNA-214表达的生物学功能与调控机制及临床应用探讨

发布时间：2018-01-28 06:02

本文关键词： microRNA- 膀胱癌生物学功能调控机制应用探讨　出处：《中华肿瘤防治杂志》2016年S2期 　论文类型：期刊论文

【摘要】：目的分析microRNA-214在膀胱癌中表达的生物学功能、调控机制以及临床应用探讨。方法选取我院2014-2015年收治的膀胱癌120例患者作为研究对象,设为实验组,取120例患者的膀胱癌新鲜冰冻组织,其中包括40例NMIBC,80例MIBC,选取120例正常人作为对照组。采用qRT-PCR方法对两组患者中的生化上皮细胞以及两种膀胱癌细胞系中的表达情况,采用体外用脂体2000将miR-214模拟物进行转染至膀胱癌细胞中,设计阴性对照组和空白对照组。采用qRT-PCR方法评估各组转染效率。利用综合生物学信息分析平台,联合五种计算方法,寻找miR-214的靶基因,并且采用双荧光素酶报告系统进行验证。采用siRNA对膀胱癌细胞系中的靶基因的表达水平进行干扰。结果 miR-214在两种膀胱癌细胞中的表达与生化上皮细胞比较明显下降,P0.05。对患者进行随访发现,120例患者中有66例膀胱癌复发,复发率为55%。总生存患者72例,总生存率为60%。结论 miR-214分析在膀胱癌组织中出现表达水平下降,与膀胱癌负面性临床病例特征有密切联系,提示膀胱癌疾病的发生和发展,在其过程中发挥重要的作用。MiR-214在膀胱癌疾病中发挥抑制癌基因的作用,抑制膀胱癌细胞的增值和迁移,促进癌细胞凋亡。
[Abstract]:Objective to analyze the biological function of microRNA-214 expression in bladder cancer. Methods 120 patients with bladder cancer admitted in our hospital from 2014 to 2015 were selected as the experimental group. Fresh frozen tissues were collected from 120 patients with bladder cancer, including 40 patients with NMIBCU and 80 patients with MIBC. The expression of biochemical epithelial cells and two kinds of bladder cancer cell lines in two groups were detected by qRT-PCR method. MiR-214 mimics were transfected into bladder cancer cells by liposome 2000 in vitro. The negative control group and the blank control group were designed. The transfection efficiency of each group was evaluated by qRT-PCR method. Looking for the target gene of miR-214. The expression level of target gene in bladder cancer cell line was interfered by siRNA. The expression of miR-214 in two kinds of bladder cancer cells was significantly lower than that in biochemical epithelial cells. P0.05. 66 of 120 patients with bladder cancer recurred and the recurrence rate was 555.The total survival rate was 72 patients. The overall survival rate is 60. Conclusion the expression level of miR-214 in bladder cancer is decreased, which is closely related to the negative clinical characteristics of bladder cancer, suggesting the occurrence and development of bladder cancer disease. MiR-214 plays an important role in inhibiting oncogene, inhibiting proliferation and migration of bladder cancer cells, and promoting apoptosis of bladder cancer cells.
【作者单位】：郑州大学第二附属医院;
【分类号】：R737.14
【正文快照】： 中华肿瘤防治杂志,2016,23(S2):11-12膀胱癌的临床发病率非常高,致死率极高。膀胱癌致病因素非常多,病理变化也复杂多变,其中吸烟和长期暴露在化学品中是导致膀胱癌的主要发病因素,吸烟时间的长短和强度与膀胱癌疾病的发生呈正相关[1-2]。膀胱癌的分期能够充分反映肿瘤浸润深

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