染料木黄酮对体外培养乳鼠睾丸组织全基因表达谱的影响

发布时间：2018-01-28 10:35

本文关键词： 全基因表达谱染料木黄酮睾丸体外培养　出处：《四川大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的利用基因芯片技术研究染料木黄酮(Genistein,Gen)对体外培养的小鼠睾丸组织全基因表达谱的影响,从基因水平分析Gen影响睾丸细胞增殖凋亡的作用机制。方法利用旋转通气法体外培养乳鼠睾丸组织,给予5μmol/L Gen干预,同时设立DMSO溶剂对照组。培养72h后提取组织总RNA,用单标Agilent小鼠全基因芯片检测两组睾丸组织基因表达,筛选差异基因,进行基因功能分类。结果与对照组比较,Gen组筛选出84个表达差异有统计学意义的基因(P0.05),其中47个基因表达上调,37个基因表达下调。按差异基因功能富集分析(GO)分类标准进行分类,筛选出有统计学意义的GO分类14个(P0.05),主要涉及生殖发育、细胞增殖、细胞周期等功能。结论通过全基因芯片检测发现,Gen主要影响睾丸组织生殖发育、细胞增殖和细胞周期等功能相关基因表达,从而影响精子发生和睾丸细胞的增殖和凋亡,影响睾丸的功能。
[Abstract]:Objective to study the effect of Genistein Genin on the gene expression profile of mouse testis in vitro. Methods testicular tissue of neonatal rats was cultured by rotating ventilation in vitro and treated with 5 渭 mol/L Gen. At the same time, the DMSO solvent control group was set up. After 72 hours of culture, the total RNAs were extracted. The gene expression in testis of two groups was detected by single standard Agilent mouse whole gene chip, and the differentially expressed genes were screened. Results compared with the control group, 84 genes with significant difference in gene expression were screened out, among which 47 genes were up-regulated. The expression of 37 genes was down-regulated. According to the criteria of differential gene function enrichment analysis, 14 (P0.05) of go classification were selected, which were mainly related to reproductive development. Conclusion Genistein mainly affects the reproductive development of testis, cell proliferation and cell cycle related gene expression. Thus affecting spermatogenesis and testicular cell proliferation and apoptosis, affecting testicular function.
【作者单位】：四川大学华西公共卫生学院营养食品卫生与毒理学系;泰山医学院附属医院营养科;
【基金】：国家自然科学基金(No.81072309)资助
【分类号】：R697
【正文快照】： 染料木黄酮(Genistein,Gen)是一种异黄酮类植物雌激素,也是大豆异黄酮的主要生物活性成分[1]。有研究报道Gen可能干扰雄性生殖发育,对雄性生殖功能产生负面影响[2]。现有的关于Gen对雄性生殖系统的影响及相关机制的研究主要关注生殖器官的形态变化、激素的合成及相关蛋白的表

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