骨髓间充质干细胞即刻与延时治疗修复阴茎海绵体神经损伤大鼠勃起功能的研究

发布时间：2018-01-30 03:06

本文关键词： 骨髓间充质干细胞勃起功能障碍海绵体神经大鼠　出处：《中华男科学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:拟观察在双侧阴茎海绵体神经(CN)损伤的大鼠神经性勃起功能障碍(NED)模型中即刻和延时向阴茎海绵体注射骨髓间充质干细胞(BM-MSCs)对大鼠阴茎勃起功能的修复作用。方法:选取28只8周龄、体重200~250 g雄性SD大鼠随机分为4组:假手术组找到双侧海绵体神经后不做处理直接关腹并缝合皮肤;对照组、即刻治疗组和延时治疗组均通过钳夹的方式损伤双侧阴茎海绵体神经建立NED模型随后关腹缝合。假手术组和对照组向阴茎海绵体内注射对照剂,即刻治疗组注射BM-MSCs,延时治疗组术后两周注射BM-MSCs。术后12周采用电刺激CN记录阴茎海绵体内压(ICP),颈动脉穿刺测定平均动脉压(MAP),ICP/MAP作为勃起功能的评价指标来评估大鼠的勃起功能。在勃起功能检测后处死大鼠,取阴茎海绵体中段组织检测平滑肌、胶原和神经纤维。结果:在手术后12周,即刻治疗组和延时治疗组ICP和ICP/MAP均较对照组升高(P0.05)。即刻治疗组和延时治疗能提高大鼠海绵体组织内平滑肌与胶原的比例(P0.05),同时两组阴茎海绵体内平滑肌含量高于对照组(P0.05),阴茎海绵体背神经内神经微丝(NF)蛋白阳性神经纤维数目和nNOS的表达高于对照组(P0.05)。结论:阴茎海绵体内注射BM-MSCs能对双侧CN损伤大鼠的勃起功能恢复有促进作用。BM-MSCs治疗可以提高海绵体组织内平滑肌与胶原的比例,改善纤维化,并能提高海绵体组织中平滑肌含量、阴茎背神经的神经丝含量和nNOS的表达水平,术后延时治疗同样能取得一定的治疗效果。
[Abstract]:Objective: to observe the injection of bone marrow mesenchymal stem cells (BMSCs) into the cavernous corpus cavernosum of the rat model of neuroerectile dysfunction (NED) in rats with bilateral cavernous nerve injury. Methods: 28 rats (8 weeks old) were selected to repair the penile erectile function. Male SD rats weighing 200 ~ 250g were randomly divided into 4 groups: sham operation group found bilateral cavernous nerves and closed abdomen and sutured skin without treatment; In the control group, immediate treatment group and delayed treatment group, the bilateral cavernous nerve was injured by clamp to establish NED model and then closed abdominal suture. The sham operation group and control group were injected into the corpus cavernosum. BM-MSCs was injected into the immediate treatment group, and BM-MSCs was injected into the delayed treatment group two weeks after operation. ICP was recorded by electrical stimulation of CN 12 weeks after operation. The average arterial pressure (MAPP / map) was measured by carotid puncture to evaluate the erectile function of rats. The rats were killed after the examination of erectile function. Smooth muscle collagen and nerve fibers were measured in the middle part of the corpus cavernosum. Results: 12 weeks after operation. ICP and ICP/MAP in immediate treatment group and delayed treatment group were higher than those in control group (P 0.05). The ratio of smooth muscle to collagen in cavernous tissue was increased in immediate treatment group and delayed treatment group (2). P0.05). At the same time, the content of smooth muscle in corpus cavernosum of both groups was higher than that of control group (P0.05). The number of positive nerve fibers and the expression of nNOS in the dorsal cavernous nerve of the penis were higher than those in the control group (P0.05). ... conclusion:. Intracavernous injection of BM-MSCs can promote the recovery of erectile function in bilateral CN injured rats. BM-MSCs can improve the ratio of smooth muscle to collagen in cavernous tissue. It can improve fibrosis and increase the content of smooth muscle in cavernous body, the content of neurofilament and the expression of nNOS in dorsal penile nerve.
【作者单位】：东南大学附属中大医院泌尿外科;
【基金】：国家自然科学基金(81300472)~~
【分类号】：R698
【正文快照】： Supported by a grant from National Natural Science Foundation of China(81300472).Correspondence to:CHEN Ming,email:mingchen0712@hotmail.comReceived:November 25,2016;accepted:February 16,2017阴茎海绵体神经(cavernous nerves,CN)是诱导阴茎勃起的主要神经,CN从

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