小鼠不同部位体内前列腺癌骨转移模型的建立及方法改进

发布时间：2018-01-30 16:25

本文关键词： 前列腺肿瘤模型动物四肢脊柱骨转移　出处：《北京大学学报(医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的:构建并改进前列腺癌(prostate cancer,PCa)体内股骨及脊柱转移动物模型,为不同部位的PCa骨转移的诊治及骨应力改变的研究提供工具。方法:将不同浓度的PCa细胞(RM-1)注射到小鼠(C57BL/6)股骨骨髓腔和腰椎内,观察记录小鼠活动变化、成瘤情况,测定瘤体大小及记录存活时间。取模型的双侧股骨组织,进行影像学检查后测算灰度值(骨密度),并行组织病理学检查,确定建模情况及成瘤类型。结果:各细胞浓度梯度小鼠均成瘤,各股骨转移模型的肿瘤发生时间、生长速度同组间无差别(P0.05),病理学检测结果证实所有股骨骨髓腔和腰椎内PCa骨转移模型构建成功,且较低RM-1细胞浓度构建的PCa骨转移动物模型生存时间较前期构建的动物模型明显延长(约1倍,至少2周)。结论:成功用同一PCa细胞株构建了不同部位(四肢骨和中轴骨)的PCa骨转移体内动物模型,经股骨骨髓腔穿刺注射肿瘤细胞建立PCa骨转移动物模型成瘤率高,肿瘤生长迅速,且可稳定复制,为进一步PCa骨转移的骨应力改变及防治的研究提供平台。
[Abstract]:Objective: to establish and improve the animal model of femoral and spinal metastasis in prostate cancer. To provide a tool for the diagnosis and treatment of PCa bone metastasis and the study of bone stress changes in different sites. Methods: different concentrations of PCa cells were injected into C57BL / 6 mice. Femoral medullary cavity and lumbar vertebrae. The changes of activity, tumorigenesis, tumor size and survival time were observed and recorded. The bilateral femur tissues of the model were taken and the gray values (bone density and histopathology) were measured after imaging examination. Results: each cell concentration gradient mice were tumorigenic, and the tumorigenesis time and growth rate of each femur metastasis model were not different (P0.05). Pathological findings confirmed that all femoral medullary and lumbar PCa bone metastasis models were successfully constructed. The survival time of PCa bone metastasis animal model with low RM-1 cell concentration was significantly longer than that of the previous model (about twice as long). Conclusion: the animal model of PCa bone metastasis in different parts (limb bone and midaxial bone) was successfully constructed with the same PCa cell line. The animal model of PCa bone metastasis was established by injecting tumor cells through femoral medullary cavity with high tumorigenesis rate, rapid tumor growth and stable replication. To provide a platform for further study of bone stress change and prevention and treatment of PCa bone metastasis.
【作者单位】：云南省第一人民医院昆明理工大学附属医院泌尿外科;云南省第一人民医院昆明理工大学附属医院康复科;中山大学附属第一医院泌尿外科;
【基金】：云南省医疗卫生单位内设研究机构科研项目(2014NS250,2014NS251) 云南省省级人培项目(KKSY201360115)资助~~
【分类号】：R-332;R737.25
【正文快照】： 前列腺癌(prostate cancer,PCa)是男性泌尿生殖系统最常见的恶性肿瘤之一[1-2],也是欧美国家男性常见的恶性肿瘤,位居美国男性恶性肿瘤发病率榜首[3]。根据世界卫生组织GLOBOCAN 2008数据(全球流行病统计数据)显示,至2008年,PCa发病率仅次于肺癌,在全球男性恶性肿瘤中位居第二

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