全反维甲酸协同增强阿霉素对去势抵抗前列腺癌的疗效

发布时间：2018-02-10 00:31

本文关键词： 全反式维甲酸阿霉素联合用药去势抵抗前列腺癌　出处：《生物技术》2017年03期 　论文类型：期刊论文

【摘要】：[目的]探究全反维甲酸与阿霉素联用对去势抵抗前列腺癌的疗效。[方法]以PC3细胞作为去势抵抗前列腺癌细胞模型,MTT法检测联合用药对细胞的增殖抑制效果;流式细胞仪检测联合用药对细胞凋亡和细胞周期的影响;反转录PCR检测联合用药对凋亡相关基因表达的影响。[结果]2μmol/L全反维甲酸与80 nmol/L阿霉素联用协同增强对PC3细胞的抑制效果(为51.2±1.41%),与单独用药存在显著性差异(P0.05),促进PC3细胞的凋亡(为17.80±0.54%),同时阻滞细胞的G1和G2期,上调Bax及caspase 3基因的表达,下调Bcl-2基因的表达。[结论]全反维甲酸与阿霉素联用可增强对PC3细胞的增殖抑制和凋亡效果,Bcl-2、Bax及caspase 3基因参与了联合用药诱导PC3细胞凋亡的调控,从而增强对PC3细胞的疗效。
[Abstract]:[objective] to investigate the efficacy of total retinoic acid (TRA) combined with doxorubicin (ADM) in the treatment of prostate cancer. [methods] PC3 cells were used as a model of castration against prostate cancer to detect the inhibitory effect of combined drug on the proliferation of prostate cancer cells. The effect of combined medication on apoptosis and cell cycle was detected by flow cytometry. The effect of reverse transcription PCR assay combined with drug therapy on the expression of apoptosis-related genes. [results] 2 渭 mol/L total retinoic acid combined with 80 nmol/L adriamycin synergistically enhanced the inhibitory effect on PC3 cells (51.2 卤1.41, P 0.05). The apoptosis of PC3 cells (17.80 卤0.54) blocked the G1 and G2 phases of the cells at the same time. [conclusion] Total retinoic acid combined with doxorubicin can enhance the proliferation inhibition and apoptosis of PC3 cells. Bcl-2 Bax and caspase 3 genes participate in the regulation of PC3 cell apoptosis induced by combined drug therapy. [conclusion] Total retinoic acid combined with doxorubicin can enhance the proliferation and apoptosis of PC3 cells. In order to enhance the efficacy of PC3 cells.
【作者单位】：南开大学生命科学学院生物活性材料教育部重点实验室;
【基金】：国家自然科学基金项目(“双重环境敏感的血管靶向性药物载体的构建及其性能研究”,No.51173083)
【分类号】：R737.25

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