大鼠hs-CRP水平与动脉粥样硬化性肾病病程的关系

发布时间：2018-02-15 12:49

本文关键词： 动脉粥样硬化肾病超敏C反应蛋白大鼠　出处：《青海大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的研究主要通过构建动脉粥样硬化性肾病模型，分别检测两组大鼠hs-CRP及肾功能（BUN、CREA）水平，了解大鼠hs-CRP水平与动脉粥样硬化性肾病病程的关系。方法选取40只健康成年雄性Wistar大鼠，将其随机分为2组：对照组和模型组（动脉粥样硬化性肾病组，AS组），对照组20只，模型组20只，造模组采用高脂饲料喂养+维生素D3腹腔注射方法构建动脉粥样硬化性肾病大鼠模型，造模周期为12周。各组分别于造模0周、造模6周、造模12周采集血标本测定血脂四项、肾功能（肌酐、尿素氮）及hs-CRP水平；造模结束后处死大鼠，制作主动脉、肾动脉及肾组织切片观察病理变化。结果造模6周及12周模型组血清hs-CRP水平分别为0.015±0.002、0.032±0.006，均高于对照组（P0.05）；6周模型组尿素氮及肌酐分别为10.33±0.81、46.8±1.3,12周模型组尿素氮及肌酐分别为12.58±0.48、72.4±2.2，均明显高于对照组（P0.05）；且6周和12周hs-CRP与BUN、CREA均呈显著正相关，6周r分别为0.606、0.947（P0.01），12周r分别为0.911、0.945（P0.01）。结论同一时期中，模型组较对照组血清hs-CRP含量增高，hs-CRP水平与肾功能指标呈显著正相关，随着hs-CRP的升高，血清尿素氮、肌酐含量随之升高，hs-CRP水平与动脉粥样硬化性肾病病程相关，hs-CRP值越高病情越重。
[Abstract]:Objective to study the relationship between hs-CRP level and the course of atherosclerotic nephropathy (Atherosclerosis nephropathy) in two groups by measuring the levels of hs-CRP and renal function BUNCREAA. Methods Forty healthy adult male Wistar rats were randomly divided into two groups: control group and model group (atherosclerotic nephropathy group, control group, control group, model group, n = 20). The rat model of atherosclerotic nephropathy was established by intraperitoneal injection of vitamin D _ 3 with high fat diet. The model period was 12 weeks. The blood samples were collected at 0 weeks, 6 weeks and 12 weeks to determine the blood lipid in each group. Renal function (creatinine, urea nitrogen) and hs-CRP level, the rats were killed after model making, and the pathological changes of aorta, renal artery and renal tissue were observed. Results the serum hs-CRP levels in the model group were 0.015 卤0.002 卤0.032 卤0.006, respectively, which were higher than those in the control group at 6 weeks (P 0.05) and the levels of urea nitrogen and creatinine in the model group were 10.33 卤0.81C, 46.8 卤1.3N and 12.58 卤0.4872.4 卤2.2respectively, which were significantly higher than those in the control group at 6 weeks and 6 weeks, respectively, and the levels of creatinine in the model group were significantly higher than those in the control group at the 6th week and the 6th week, and the creatinine levels in the model group were 12.58 卤0.4872.4 卤2.2, respectively. At 12 weeks, there was a significant positive correlation between hs-CRP and BUNA at 6 weeks (r = 0. 606, P 0. 047, P 0. 01, P 0. 01, r = 0. 911, P 0. 045, P 0. 01, r = 0. 911, P 0. 01, P 0. 01, respectively). Conclusion in the same period, the level of serum hs-CRP in the model group was significantly higher than that in the control group, and the level of hs-CRP was positively correlated with the renal function index. With the increase of hs-CRP, the serum urea nitrogen level was significantly increased. Creatinine content increased and the level of hs-CRP was associated with the course of atherosclerotic nephropathy. The higher the level of hs-CRP was, the more serious the disease was.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R543.5;R692

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