促红素对高糖诱导足细胞肾素受体表达的影响

发布时间：2018-02-16 08:35

本文关键词： 促红细胞生成素高糖足细胞肾素受体　出处：《中国糖尿病杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的研究促红细胞生成素(EPO)对高糖培养小鼠肾脏足细胞肾素受体(RR)表达的影响。方法体外培养和分化小鼠肾小球足细胞,按培养条件不同分为正常对照组(Con,含5.6mmol/L葡萄糖),高渗组(含5.6mmol/L葡萄糖+24.4mmol/L甘露醇),高糖1组(含15mmol/L葡萄糖),高糖2组(含30mmol/L葡萄糖)。每个培养条件分别以0、10、20、50U/ml的EPO进行干预,干预24h后收集足细胞。RT-PCR、Western blot检测RR表达情况。结果不同葡萄糖浓度处理后,高糖1组、高糖2组足细胞RR mRNA和蛋白表达相对量与Con组比较,差异有统计学意义(P0.05)。不同EPO浓度干预各组足细胞24h后,各组均在10U/ml EPO浓度时,足细胞RR mRNA和蛋白表达最低,与各组不加EPO干预比较,差异有统计学意义(P0.05)。结论 EPO对高糖诱导的足细胞RR mRNA及蛋白过表达有调节作用。
[Abstract]:Objective to study the effect of erythropoietin (EPO) on the expression of renin receptor (RRR) in renal podocytes of mice cultured with high glucose. Methods the rat glomerular podocytes were cultured and differentiated in vitro. They were divided into normal control group with 5.6 mmol / L glucose, hyperosmotic group (5.6 mmol / L glucose 24.4 mmol / L mannitol), high glucose group 1 (containing 15 mmol / L glucose) and high glucose group 2 (containing 30 mmol / L glucose). Each culture condition was treated with 0 10 mmol / L glucose / L 50 U / ml EPO, respectively. Results the relative amount of RR mRNA and protein expression in high glucose 1 group and high glucose 2 group was compared with that in Con group. The expression of RR mRNA and protein in podocytes was the lowest at the concentration of 10 U / ml EPO after 24 hours of intervention with different concentrations of EPO, and compared with that of each group without the intervention of EPO. Conclusion EPO can regulate the overexpression of RR mRNA and protein in podocytes induced by high glucose.
【作者单位】：青岛市市立医院肾内科;青岛市市立医院中心实验室;
【分类号】：R587.2;R692.9

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