吲哚胺2,3-双加氧酶及叉头螺旋转录因子在儿童肾母细胞瘤中的表达及意义

发布时间：2018-02-24 10:22

本文关键词： 肾母细胞瘤 IDO FoxP3 肿瘤免疫　出处：《重庆医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：通过检测IDO、FoxP3在儿童肾母细胞瘤组织中的表达，分析其与肾母细胞瘤临床病理学参数的相关性，研究IDO、FoxP3在肾母细胞瘤免疫耐受过程中的作用，并探讨二者能否作为判断儿童肾母细胞瘤预后的指标。方法：回顾性分析重庆医科大学附属儿童医院泌尿外科2010.7-2011.8期间诊断为肾母细胞瘤的临床资料，，包括患儿性别、年龄、临床分期、病例类型，应用免疫组织化学法对IDO和FoxP3在儿童肾母细胞瘤中的表达进行定位和半定量分析，并将其与患儿性别、发病年龄、肿瘤临床分期、病理类型进行相关性分析。结果：20例肾母细胞瘤中有10例IDO呈阳性表达，阳性表达率为50%。6例FoxP3阳性表达，阳性表达率为30%。IDO和FoxP3在同一样本中均阳性表达的有5例，阳性率为25%。20例中有9例IDO和FoxP3均为阴性。同一样本中IDO阳性和FoxP3阴性者5例（25%），同一样本中IDO阴性和FoxP3阳性者1例（20%）; IDO、FoxP3的表达与肾母细胞瘤发病年龄、患儿性别、肿瘤临床分期、病理类型均无显著相关性（P＞0.05）；IDO和FoxP3二者的表达具有正相关性（P＜0.05）；同时表达IDO和FoxP3的肿瘤在患儿性别、年龄、肿瘤临床分期、病理类型等方面均无显著差异（P＞0.05）。20例肾母细胞瘤中1例复发，但其IDO、FoxP3表达均为阴性。结论：IDO和FoxP3在儿童肾母细胞瘤中阳性率低，提示二者可能不参与肾母细胞瘤免疫耐受的形成，其原因可能是肾母细胞瘤作为一种胚胎性肿瘤，与成人肿瘤发病机制不同。IDO的表达与FoxP3的表达呈正相关，提示在肾母细胞瘤中IDO和FoxP3+Tregs之间可能存在相互作用。因本研究样本量有限，IDO和FoxP3在肾母细胞瘤免疫耐受中的作用尚需进一步验证，其能否作为判断儿童肾母细胞瘤预后的临床指标还有待深入研究。
[Abstract]:Objective: to study the role of IDO- FoxP3 in immunological tolerance of nephroblastoma by detecting the expression of IDO- FoxP3 in children's nephroblastoma, and analyzing its correlation with clinicopathological parameters of nephroblastoma. To explore whether they can be used as a prognostic index for children with nephroblastoma. Methods: the clinical data of nephroblastoma diagnosed in Urology Department, affiliated Children's Hospital of Chongqing Medical University, were retrospectively analyzed, including sex, age, clinical stage and type of cases. The expression of IDO and FoxP3 in children's nephroblastoma was determined by immunohistochemical method. The expression of IDO and FoxP3 was correlated with sex, age, clinical stage and pathological type of children. Results among 20 cases of nephroblastoma, 10 cases were positive for IDO, and the positive expression rate was 50.6 cases. The positive expression rate of 30. IDO and FoxP3 in the same sample was 5 cases. The positive rate of IDO and FoxP3 was negative in 9 out of 25. 20 cases. In the same sample, 5 cases were IDO positive and FoxP3 negative, 1 case was IDO negative and 1 case was FoxP3 positive in the same sample, and the expression of IDOX FoxP3 was associated with the onset age, sex, clinical stage of tumor. There was no significant correlation between the expression of IDO and FoxP3 in pathological types (P > 0.05) and the positive correlation between the expression of IDO and FoxP3 (P < 0.05), and the expression of IDO and FoxP3 in children with sex, age, clinical stage of tumor. There was no significant difference in pathological types (P > 0.05). One of 20 cases of nephroblastoma recurred, but the expression of FoxP3 was negative. Conclusion the low positive rate of FoxP3 and VIDO in children nephroblastoma suggests that they may not participate in the formation of immune tolerance in nephroblastoma, which may be due to the fact that Wilms tumor is an embryonic tumor. The expression of. IDO was positively correlated with the expression of FoxP3, which was different from the pathogenesis of adult tumors. It is suggested that there may be interaction between IDO and FoxP3 Tregs in nephroblastoma. The role of IDO and FoxP3 in immunological tolerance of nephroblastoma needs to be further verified. Whether it can be used as a clinical index to judge the prognosis of child nephroblastoma remains to be further studied.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.11

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