Wnt-β-catenin信号分子在急性肾损伤大鼠肾组织中的表达及意义

发布时间：2018-02-25 17:36

本文关键词： Wnt-β-catenin信号分子急性肾损伤再灌注损伤信号通路　出处：《中国免疫学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨Wnt-β-catenin信号分子在急性肾损伤大鼠肾组织中的表达。方法:将48只大鼠按照随机数字表法分为正常对照组(Control组)、假手术组(Sham组)和缺血再灌注组(IRI组),每组16只;Control组正常喂养,IRI组建立大鼠缺血再灌注损伤模型,Sham组背侧切开后直接将伤口关闭。分别于术后第1天、第3天、第5天、第7天处死4只大鼠,观察三组大鼠肾脏组织病理学改变,并检测三组大鼠血肌酐、尿素氮水平;采用免疫印迹法检测三组大鼠肾脏组织Wnt4、β-catenin、Lrp6蛋白表达,实时定量RT-PCR检测肾脏组织Wnt4、β-catenin、Lrp6 mRNA表达。结果:Control组、Sham组各时段肾脏损伤评分、血肌酐比较差异无统计学意义(P0.05);IRI组术后第1天至第7天肾脏损伤评分、血肌酐、尿素氮先上升后逐渐降低,其中术后第3天肾脏损伤评分、肌酐、尿素氮最高;IRI组术后1、3、5、7 d肾脏损伤评分、血肌酐、尿素氮显著高于Control组、Sham组(P0.05)。Control组、Sham组各时段Wnt4、β-catenin、Lrp6蛋白表达差异无统计学意义(P0.05);IRI group术后Wnt4、β-catenin、Lrp6蛋白逐渐增加,至术后第5天达表达高峰,随后逐渐降低;IRI组术后1、3、5、7 d Wnt4、β-catenin、Lrp6蛋白均显著高于Control组、Sham组(P0.05);Control组、Sham组各时段Wnt4、β-catenin、Lrp6 mRNA表达差异无统计学意义(P0.05);IRI group术后Wnt4、β-catenin、Lrp6 mRNA显著增加,至术后第3天达表达高峰,随后逐渐降低;IRI组术后1、3、5、7 d Wnt4、β-catenin、Lrp6 mRNA均显著高于Control组、Sham组(P0.05)。结论:Wnt-β-catenin信号分子在急性肾损伤组织中表达显著增加,激活的Wnt-β-catenin信号通路参与了肾脏组织的修复过程。
[Abstract]:Objective: to investigate the expression of Wnt- 尾 -catenin signal molecules in renal tissue of rats with acute renal injury. Methods: 48 rats were randomly divided into control group (control group), sham-operated group (Sham group) and ischemia-reperfusion group (IRI group). 16 rats in control group were fed with IRI group to establish the model of ischemia-reperfusion injury. The wound was closed directly after dorsal incision in Sham group, respectively on the 1st day after operation. On day 3, day 5 and day 7, four rats were killed to observe the histopathological changes of kidney in the three groups, and the levels of serum creatinine and urea nitrogen in the three groups were detected, and the expression of Wnt4, 尾 -cateninine Lrp6 protein in the renal tissues of the three groups was detected by Western blot. Real time quantitative RT-PCR was used to detect the expression of Wnt4, 尾 -cateninine Lrp6 mRNA in renal tissue. Results there was no significant difference in renal injury score and serum creatinine between 1 and 7 days after operation in the control group. The renal injury score, creatinine and serum creatinine in the IRI group were increased at first and then decreased gradually at the 3rd day after operation, and the renal injury score and serum creatinine in the IRI group were higher than those in the IRI group on the 3rd day after operation. There was no significant difference in the expression of Wnt4, 尾 -cateninine Lrp6 protein between Control group and Sham group (P 0.05), and the expression of 尾 -cateninine lrp6 protein increased gradually after IRI group, and reached the peak at the 5th day after operation. Then the expression of Wnt4, 尾 -cateninine lrp6 protein in Control group was significantly higher than that in Sham group (P 0.05), and there was no significant difference in the expression of Wnt4, 尾 -catenin Control Lrp6 mRNA, 尾 -cateninine Control Lrp6 mRNA after group, and the expression of Wnt4, 尾 -cateninine lrp6 mRNA increased significantly after group operation, and the expression of Wnt4 and 尾 -cateninine lrp6 mRNA increased significantly at the third day after operation, and the expression of Wnt4 and 尾 -catenininine Lrp6 mRNA reached the peak at the 3rd day after operation. Subsequently, Wnt4, 尾 -catenin Lrp6 mRNA in IRI group was significantly higher than that in Control group (P 0.05). Conclusion the expression of Wnt- 尾 -catenin signal molecules in acute renal injury tissues was significantly increased, and the activated Wnt- 尾 -catenin signaling pathway was involved in the process of renal tissue repair.
【作者单位】：厦门大学附属第一医院肾脏内科;厦门大学附属第一医院皮肤科;
【分类号】：R692

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