miRNA-196a2及miRNA-499基因多态性与膀胱癌的相关性研究

发布时间：2018-03-03 02:35

本文选题：膀胱癌　切入点：微小RNA　出处：《医学研究生学报》2015年03期 　论文类型：期刊论文

【摘要】：目的微小RNA(microRNA,miRNA)基因多态性与肿瘤的关系密切。文中探讨rs11614913(miRNA-196a2)及rs3746444(miRNA-499)的基因多态性与膀胱癌及其病理参数的相关性。方法选取膀胱癌患者283人作为膀胱癌组,正常体检者283人作为正常对照组。利用Massarray单核苷酸多态性检测技术对膀胱癌组和正常对照组的rs11614913(miRNA-196a2)及rs3746444(miRNA-499)基因多态性进行分析;采用Logistic回归模型分析各基因型与膀胱癌发生的关系并比较不同基因型与膀胱癌及其病理参数的关系。结果 rs11614913位点的各基因型在2组中分布差异有统计学意义(χ2=15.077,P0.001),其中TT基因型在膀胱癌组中的频率(19.08%)显著低于对照组(33.95%),差异有统计学意义(P0.05);TT基因型发生膀胱癌的风险是CC基因型的0.45倍(ORadjusted=0.45,95%CI:0.28~0.73)。rs3746444位点的各基因型在2组中分布差异无统计学意义(χ2=5.107,P=0.078),但GG基因型在膀胱癌组中分布频率(7.77%)显著高于对照组(3.56%),差异有统计学意义(P0.05);GG基因型发生膀胱癌的风险是AA的2.39倍(ORadjusted=2.39,95%CI:1.10~5.17)。rs11614913及rs3746444位点的基因型分布频数在不同肿瘤分期、周围淋巴结转移、远端淋巴结转移组间的差异无统计学意义(P0.05)。然而,在不同分化程度的肿瘤分组中2个位点的基因型分布频数差异有统计学意义(rs11614913:χ2=15.066,P=0.005;rs3746444:χ2=11.120,P=0.025)。结论 rs11614913(miRNA-196a2)及rs3746444(miRNA-499)基因多态性与膀胱癌的发生相关,2个位点的基因多态性与肿瘤的分化程度相关。
[Abstract]:Objective to investigate the association between microRNA-miRNA-499) gene polymorphisms and bladder cancer and its pathological parameters. Methods 283 patients with bladder cancer were selected as bladder cancer group, and the relationship between the polymorphism of rs11614913 miRNA-196a2) and rs3746444 miRNA-499) gene polymorphism was studied. The rs11614913 miRNA-196a2 and rs3746444miRNA-499) gene polymorphisms were detected by Massarray single nucleotide polymorphisms (SNP) in normal controls. Logistic regression model was used to analyze the relationship between genotypes and bladder carcinogenesis, and to compare the relationship between different genotypes and bladder cancer and its pathological parameters. Results there were significant differences in the distribution of rs11614913 loci between the two groups (蠂 2 = 15.07 7, P 0.001). The frequency of TT genotype in bladder cancer group (19.08) was significantly lower than that in control group (33.95%). The difference was statistically significant. The risk of bladder cancer of TT genotype was 0.45 times higher than that of CC genotype. The frequency of GG genotype distribution in bladder cancer group was significantly higher than that in control group (7.77). There was a statistically significant difference in the risk of bladder cancer with P0.05 GG genotype. The risk of bladder cancer was 2.39 times higher than that of AA, and the distribution frequency of GG genotype was 2.39 times higher than that of AA, and the distribution frequency of Rs11614913 and rs3746444 locus was different in different stages of the tumor, and the frequency of GG genotype distribution was significantly higher than that of control group (P < 0.05), but the distribution frequency of GG genotype in bladder cancer group was significantly higher than that in control group (P = 3.56). The risk of GG genotype was 2.39 times higher than that of AA. There was no significant difference between the peripheral lymph node metastasis group and the distal lymph node metastasis group (P 0.05). There were significant differences in the genotypic frequencies of two loci in different differentiated tumor groups. There were significant differences in the genotypic frequencies of two loci: rs11614913: 蠂 ~ 2 ~ 2 ~ (15.0666) P ~ (0.005) rs37466 ~ (4): 蠂 ~ (2) ~ (2) ~ (11.120) (P ~ (0.02525)). Conclusion the polymorphism of rs11614913 miRNA-196a2 and rs3746444miRNA-499) gene polymorphisms are associated with the development of bladder cancer, and the gene polymorphisms of two loci are related to the differentiation degree of the tumor.
【作者单位】：南京医科大学附属南京医院泌尿外科;
【基金】：南京医科大学科技发展基金(2010NJMU241) 南京市医学科技发展项目(YKK11116,QRX11251)
【分类号】：R737.14

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