高糖诱导肾小管上皮细胞发生上皮-间质转化过程中Snail 1对IGF-1表达的影响

发布时间：2018-03-08 17:45

本文选题：糖尿病肾脏病　切入点：肾纤维化　出处：《广东医学》2017年15期 　论文类型：期刊论文

【摘要】：目的研究锌指转录因子1(Snail 1)对高糖诱导的发生上皮-间质转化(epithelial to mesenchymal transition,EMT)的大鼠肾小管上皮细胞(NRK-52E)中胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)表达的影响,探讨糖尿病肾脏病发生机制及Snail 1与自分泌IGF-1的相关性。方法大鼠近端肾小管上皮细胞系(NRK-52E),分组如下:对照组、高糖组、高糖+NT组、高糖+siRNA组。分别于培养48 h、72 h收获细胞。用免疫荧光、原位杂交等方法检测细胞Snail 1、IGF-1、E-钙黏蛋白(E-cadherin)和纤维连接蛋白(fibronectin,FN)mRNA及蛋白的表达。结果与对照组相比,高糖组细胞IGF-1、Snail 1和FN的mRNA及蛋白表达明显增加,差异有统计学意义(P0.01)。E-cadherin的mRNA及蛋白表达明显减少,差异有统计学意义(P0.01);与高糖组相比,高糖+siRNA组细胞IGF-1、Snail 1和FN的mRNA及蛋白表达明显减少,差异有统计学意义(P0.01),E-cadherin的mRNA及蛋白表达明显增加,差异有统计学意义(P0.01);与高糖组相比,高糖+NT组IGF-1、Snail 1、E-cadherin和FN的mRNA及蛋白表达,差异无统计学意义(P0.05)。Spearman's相关性分析显示,NRK-52E细胞中Snail 1与IGF-1蛋白的表达呈正相关(r=0.936,P0.05)。结论高糖诱导肾小管上皮细胞发生EMT的过程中Snail 1可能通过调节IGF-1促进糖尿病肾脏病肾脏纤维化。
[Abstract]:Objective to study the zinc finger transcription factor 1 (Snail 1) on the occurrence of epithelial mesenchymal transition induced by high glucose (epithelial to mesenchymal transition, EMT) of the rat renal tubular epithelial cells (NRK-52E) in insulin-like growth factor -1 (insulin-like growth factor 1, IGF-1) on the expression of relationship between pathogenesis and Snail diabetes kidney disease. Methods 1 and autocrine IGF-1 rat renal proximal tubular epithelial cell line (NRK-52E), control group, high glucose group, high glucose group, high glucose group +siRNA +NT, respectively. After 48 h, 72 h. Cells were harvested by immunofluorescence and in situ hybridization were used to detect cell Snail 1, IGF-1 E-, E-cadherin (E-cadherin) and fibronectin (fibronectin, FN) mRNA and protein expression. Results compared with the control group, high glucose group were IGF-1, mRNA and protein expression of Snail 1 and FN increased significantly, the difference was statistically significant (P0.01.E-c) MRNA and protein expression of adherin was significantly reduced, the difference was statistically significant (P0.01); compared with the high glucose group, high glucose group +siRNA cells IGF-1, mRNA and protein expression of Snail 1 and FN were significantly reduced, the difference was statistically significant (P0.01), mRNA and protein expression of E-cadherin increased significantly, the difference was statistically significant (P0.01); compared with the high glucose group, high glucose group +NT IGF-1, Snail 1, mRNA and protein expression of E-cadherin and FN, there was no statistically significant difference (P0.05).Spearman's correlation analysis showed that Snail cells in NRK-52E 1 and IGF-1 protein expression were positively correlated (r=0.936, P0.05). The Snail of renal tubular epithelial cells induced by EMT conclusion high glucose in 1 by regulating IGF-1 promotes renal fibrosis in diabetic kidney disease.

【作者单位】：贵州医科大学免疫学教研室;贵州省人民医院肾内科;
【基金】：国家自然科学基金资助项目(编号:81200539) 贵州省科技厅-贵州省人民医院联合基金项目(黔科合LH字[2014]7002号,黔科合LH字[2015]7155号,黔科合LH字[2015]7154号,黔科合LH字[2016]7169号) 贵州省卫生计生委科学技术基金项目(编号:gzwkj2014-1-046)
【分类号】：R587.2;R692.9

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