肾癌组织miR-222、miR-122表达变化及其临床意义

发布时间：2018-03-09 03:32

本文选题：肾癌　切入点：微小RNA-　出处：《山东医药》2017年44期 　论文类型：期刊论文

【摘要】：目的探讨微小RNA-222(miR-222)、miR-122在肾癌发生、发展中的作用。方法选择肾癌患者72例,收集其术中切除的肾癌组织及其配对癌旁正常组织标本。采用实时荧光定量PCR法检测肾癌组织及其配对癌旁正常组织miR-222、miR-122表达,分析肾癌组织miR-222、miR-122表达与患者临床病理参数的关系及二者的相关性。结果肾癌组织及癌旁正常组织miR-222相对表达量分别为2.38±0.64、1.12±0.55,miR-122相对表达量分别为2.86±0.39、0.98±0.43;肾癌组织miR-222、miR-122相对表达量均高于癌旁正常组织(P均0.01)。肾癌组织miR-222、miR-122表达与患者性别、年龄、病理类型、肿瘤直径均无关(P均0.05),与患者病理分级、TNM分期、浸润深度、淋巴结转移均有关(P均0.01)。肾癌组织miR-222、miR-122相对表达量呈正相关(r=0.517,P0.05)。结论肾癌组织miR-222、miR-122表达均升高,二者可能协同促进肾癌的发生、发展;癌组织miR-222、miR-122表达变化有助于病情评估。
[Abstract]:Objective to study the effect of RNA-222 (miR-222) and miR-122 in renal cell carcinoma and its role in the development. Methods 72 cases of renal cell carcinoma, collecting the resection of renal carcinoma tissue and adjacent normal tissue specimens. Detected by real-time fluorescence quantitative PCR in renal cell carcinoma and adjacent normal tissue miR-222, miR-122 expression, analysis of renal cell carcinoma miR-222, the correlation relationship between miR-122 expression and clinicopathological parameters of patients and two. The results of renal cell carcinoma tissues and adjacent normal tissue miR-222 expression levels were 2.38 + 0.64,1.12 + 0.55, miR-122 expression levels were 2.86 + 0.39,0.98 + 0.43; renal cell carcinoma miR-222, miR-122 expression were higher than those in adjacent normal tissues (P 0.01). Renal cell carcinoma miR-222, miR-122 expression and gender, age, pathological type, tumor diameter were independent (P 0.05), and patients with pathological grade, TNM stage, depth of invasion, Lymph node metastasis (P < 0.01). Renal tissue miR-222, miR-122 expression was positively correlated (r=0.517, P0.05). Conclusion miR-222 in renal cell carcinoma, the expression of miR-122 was increased, the two may promote the development of cooperative, renal cancer; miR-222, miR-122 expression changes to help assess the condition.

【作者单位】：连云港市第一人民医院;
【基金】：江苏省科技计划发展项目(BK2014187)
【分类号】：R737.11

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