荧光原位杂交检测尿脱落细胞中3号、7号、17号染色体及9p16基因突变值与膀胱移行细胞癌病理分级相关性研究

发布时间：2018-03-18 18:22

本文选题：膀胱移行细胞癌　切入点：荧光原位杂交　出处：《吉林大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：利用荧光原位杂交技术，测出膀胱移行细胞癌患者尿脱落细胞中3号染色体、7号染色体、17号染色体及9号染色体p16基因突变值，探讨突变值与膀胱移行细胞癌病理分级之间是否存在相关性，为膀胱移行细胞癌的早期诊断及分级诊断提供一种新的方法。方法： 1、选取实验对象100例（2012年10月至2013年5月期间吉林大学中日联谊医院确诊膀胱占位性病变，且脱落细胞检测发现尿中存在脱落细胞）。2、进行荧光原位杂交检查，并记录尿脱落细胞中3号染色体、7号染色体、17号染色体及9号染色体p16基因突变值。3、术中留取组织标本（已获得医学伦理委员会认可），HE染色后，行组织病理学检查，确定肿瘤分级。分级标准采用WHO2004分级法。结果：FISH检测所有患者尿脱落细胞均可见有荧光标记，且3号染色体、17号染色体异常细胞个数及3号染色体、7号染色体、17号染色体异常细胞内非整倍染色体的个数（即突变值）与肿瘤病理分级呈正相关性(Sig＜0.005)；结论：3号、17号染色体异常细胞个数及3号、7号、17号染色体异常细胞内非整倍染色体的个数（即突变值）与膀胱移行细胞癌病理分级呈正相关性，9号染色体p16基因的丢失与病理分级之间无明显相关性。可认为荧光原位杂交技术检测能够区分不同病理级别的膀胱移行细胞癌，，可用于临床早期病理分级诊断。
[Abstract]:Objective: to detect the mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells of patients with transitional cell carcinoma of bladder by fluorescence in situ hybridization. To explore the correlation between mutation value and pathological grade of bladder transitional cell carcinoma (TCC), and to provide a new method for early diagnosis and grading diagnosis of TCC. Methods:. 1. From October 2012 to May 2013, 100 patients (from October 2012 to May 2013) were selected to confirm the bladder occupying lesions, and the exfoliated cells were detected by fluorescence in situ hybridization, and the exfoliated cells were found to exist in the urine. The mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells was recorded. The grade of tumor was determined. The standard of classification was WHO2004. Results the urine exfoliated cells of all the patients were detected with fluorescent markers by the urine sample. The number of abnormal cells on chromosome 3 and 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 were positively correlated with pathological grade of tumor. Conclusion: the number of abnormal cells on chromosome 3, 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 are positively correlated with the pathological grade of bladder transitional cell carcinoma. There is no significant correlation between loss and pathological grade. Fluorescence in situ hybridization can be used to distinguish transitional cell carcinoma of bladder with different pathological grades. It can be used for clinical early pathological grading diagnosis.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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