CYLD抑制自噬提高膀胱癌细胞吉西他滨化疗敏感性

发布时间：2018-03-23 15:35

本文选题：膀胱肿瘤　切入点：吉西他滨　出处：《山东大学学报(医学版)》2017年08期

【摘要】：目的探讨肿瘤抑制因子CYLD提高膀胱癌细胞吉西他滨化疗敏感性。方法构建CYLD过表达质粒转染的人膀胱癌细胞株T24、253J,将细胞分为仅加入脂质体的对照组,转染空载质粒的空载组和CYLD组。Transw ell实验检测CYLD对膀胱癌细胞迁移/侵袭能力的影响,CCK8法检测膀胱癌细胞对吉西他滨的化疗敏感性,Western blotting、吖啶橙染色、电镜等检测自噬蛋白表达水平。结果与对照组和空载组相比,CYLD组通过Transw ell小室的细胞数目明显减少(P0.05),24 h半抑制浓度(IC50)明显降低(P0.05),自噬相关蛋白LC3、Beclin 1表达降低,p62表达增高,吖啶橙染色及电镜观察发现自噬溶酶体及自噬泡减少。结论肿瘤抑制因子CYLD可以抑制膀胱癌细胞的迁移/侵袭能力,并通过抑制自噬提高膀胱癌细胞对吉西他滨的化疗敏感性。
[Abstract]:Objective to investigate the effect of tumor suppressor CYLD on the chemosensitivity of bladder cancer cell line gemcitabine. Methods A human bladder cancer cell line T24253J transfected with CYLD overexpression plasmid was constructed and divided into two groups: T24253Jcells were treated with liposome only. The effect of CYLD on the migration / invasion of bladder cancer cells was detected by ell assay. The chemosensitivity of bladder cancer cells to gemcitabine was detected by CCK8 method. Western blotting and acridine orange staining were used to detect the chemosensitivity of bladder cancer cells to gemcitabine. Results compared with control group and no-load group, the expression of autophagy protein in Cyd group decreased significantly compared with control group and no-load group. Results compared with control group and no-load group, the number of cells passing through Transw ell chamber was significantly decreased by IC50), and the expression of autophagy associated protein LC3nBeclin 1 was decreased significantly, and the expression of p62 was increased. Acridine orange staining and electron microscopy showed that autophagy lysosomes and autophagy decreased. Conclusion tumor suppressor CYLD can inhibit the migration / invasion of bladder cancer cells and increase the chemosensitivity of bladder cancer cells to gemcitabine by inhibiting autophagy.
【作者单位】：山东大学附属省立医院泌尿外科;山东大学附属生殖医院医务科;山东大学附属省立医院心内科;
【基金】：山东省自然科学基金面上项目(2014ZRB14081);山东省自然科学基金(2014ZRB14513) 山东省重点研发计划(2015GSF118055) 山东省医药卫生科技发展计划(2014WS0341)
【分类号】：R737.14

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