自制免疫磁珠在前列腺肿瘤组织干细胞提取中的应用

发布时间：2018-03-26 14:06

本文选题：肿瘤干细胞　切入点：免疫磁化分离　出处：《中国组织工程研究》2016年01期

【摘要】：背景:有效分离干细胞是前列腺癌发生发展研究的基础,目前的主流方法是免疫磁珠分选。目的:应用自制的免疫磁珠分选前列腺肿瘤组织中的CD133~+/CD44~+细胞,并进一步培养和传代进行免疫学鉴定,以确定免疫磁珠分选效果。方法:应用独创的磁珠制备免疫磁珠,分选肿瘤组织中细胞膜表达CD133和CD44的细胞。观察分选细胞无血清培养成球情况,及传代后其在形态学、增殖能力方面与标准肿瘤细胞株的差异,并用免疫荧光法检测其特异性抗体的表达。结果与结论:制备的免疫磁珠粒径小、分选效果佳。分选的细胞成球效果好,培养传代后的细胞依旧明显表达CD133和CD44抗原。分选的细胞非诱导培养后可见多种形态,生长旺盛。转化生长因子β诱导培养后细胞形态较单一且生长缓慢。两组细胞与标准肿瘤细胞株培养后的增殖情况差异均有显著性意义(P0.05)。结果表明应用自制免疫磁珠从肿瘤细胞中分选的CD133~+/CD44~+细胞经过初步形态及功能鉴定具有干细胞的特性,可为前列腺癌干细胞的提取及进一步培养研究奠定基础。
[Abstract]:Background: the effective isolation of stem cells is the basis of prostate cancer development, and the main method is immunomagnetic bead sorting. Objective: to separate CD133- / CD44~ cells from prostate cancer tissue by self-made immunomagnetic beads. In order to determine the separation effect of immunomagnetic beads, the immunomagnetic beads were prepared by using the original magnetic beads. The cell membrane expressing CD133 and CD44 in tumor tissue was selected. The granulation of the cells without serum was observed, and the morphological and proliferative abilities of the cells were compared with those of the standard tumor cell line after passage. Results and conclusion: the prepared immunomagnetic beads have small particle size and good sorting effect. The cultured cells still expressed CD133 and CD44 antigens. After induction of transforming growth factor 尾 (TGF- 尾), the cell morphology was simpler and slower. There were significant differences in proliferation between the two groups and standard tumor cell lines. The results showed that self-made immunity was used. The CD133- / CD44~ cells separated by magnetic beads from tumor cells were identified as stem cells by preliminary morphology and function. It can lay a foundation for the extraction and further culture of prostate cancer stem cells.
【作者单位】：天津医科大学第二医院药学部;天津市儿童医院检验科;天津大学药物科学与技术学院;天津医科大学第二医院泌尿外科天津市泌尿外科研究所;
【基金】：天津市卫生和计划生育委员会科技基金(2014KZ093)~~
【分类号】：R737.25

【共引文献】