荧光原位杂交技术在膀胱癌诊断中的应用

发布时间：2018-04-08 22:38

本文选题：荧光原位杂交　切入点：膀胱肿瘤　出处：《中华医院感染学杂志》2009年14期

【摘要】：目的应用染色体特异性探针对尿脱落细胞进行FISH分析膀胱移行细胞癌的染色体畸变。方法选用3、7、9、17号染色体特异性探针对50例膀胱移行细胞癌患者尿液的脱落细胞进行荧光原位杂交研究,并同时做细胞学检查。结果膀胱癌患者尿液脱落细胞中3、7、9、17号染色体数目畸变阳性率分别为28%、32%、56%和38%,其中9号染色体畸变率较高,但与膀胱癌的分级无明显相关关系;3、7和17号染色体数目畸变与膀胱癌的分期密切相关。结论膀胱癌的发生发展与染色体的畸变有关,应用FISH检测膀胱癌患者尿液细胞染色体数目畸变,可以作为膀胱癌诊断、预后判断的一项辅助方法,该方法具有快速、准确、灵敏度高的优点。
[Abstract]:Objective to analyze chromosomal aberration of bladder transitional cell carcinoma (TCC) by FISH with chromosome specific probe.Methods the exfoliated cells from 50 patients with transitional cell carcinoma of bladder were studied by fluorescence in situ hybridization with specific probes of chromosome 3 and chromosome 17, and cytological examination was also performed at the same time.Results in urine exfoliated cells of bladder cancer patients, the positive rates of chromosome 3, 7 and 17 were 2832, 56% and 38, respectively, and the rate of chromosome 9 was higher.However, there was no significant correlation between the number aberration of chromosome 37 and chromosome 17 and the stage of bladder cancer.Conclusion the occurrence and development of bladder cancer is related to chromosome aberration. The detection of chromosome aberration in urine by FISH can be used as an auxiliary method for the diagnosis and prognosis of bladder cancer. This method is rapid and accurate.The advantage of high sensitivity.
【作者单位】：第三军医大学西南医院检验科;第三军医大学西南医院泌尿外科;
【基金】：卫生部科研基金项目(WKJ2007-3-001)
【分类号】：R737.14

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