趋化因子配体10及趋化因子受体3在膀胱癌中的表达及意义

发布时间：2018-04-10 18:05

本文选题：膀胱肿瘤 + 趋化因子配体　；参考：《重庆医学》2015年25期

【摘要】：目的检测趋化因子配体10(CXCL10)及趋化因子受体3(CXCR3)在膀胱癌中的表达并探讨其临床意义。方法80例膀胱癌患者和33例健康体检者分别纳入试验组和对照组。采用ELISA检测血清中CXCL10的表达,采用逆转录-聚合酶链反应(RT-PCR)法检测外周血单一核细胞中CXCR3mRNA的表达,并将CXCL10和CXCR3的表达强度与各临床参数进行相关性分析。结果试验组外周血清中CXCL10及外周血单一核细胞中CXCR3mRNA的表达量均显著高于对照组;试验组血清CXCL10及CXCR3mRNA的表达与年龄、病理分期、分化程度、肿瘤大小等均不存在相关性(P0.05),但均与有无淋巴结转移相关(P0.05)。结论 CXCL10及CXCR3的表达与膀胱癌的发生发展及淋巴结转移可能存在相关性,阻断CXCL10/CXCR3反应轴,可能会成为膀胱癌治疗的一个新靶点。
[Abstract]:Objective to investigate the expression of chemokine ligand 10 (CXCL10) and chemokine receptor 3 (CXCR3) in bladder cancer and its clinical significance.Methods 80 patients with bladder cancer and 33 healthy controls were included in the trial group and the control group.The expression of CXCL10 in serum was detected by ELISA and the expression of CXCR3mRNA in peripheral blood mononuclear cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). The correlation between the expression of CXCL10 and CXCR3 and the clinical parameters was analyzed.Results the expression of CXCL10 in peripheral serum and CXCR3mRNA in peripheral blood mononuclear cells in the test group was significantly higher than that in the control group, and the expression of CXCL10 and CXCR3mRNA in the serum of the test group was associated with age, pathological stage and differentiation.There was no correlation between tumor size and tumor size, but there was no correlation between tumor size and lymph node metastasis.Conclusion the expression of CXCL10 and CXCR3 may be related to the occurrence and development of bladder cancer and lymph node metastasis. Blocking the reaction axis of CXCL10/CXCR3 may become a new target for the treatment of bladder cancer.
【作者单位】：海南医学院附属医院泌尿外科;中南大学湘雅医院泌尿外科;
【基金】：海南省教育厅科研项目(HNKY2014-55) 海南省科技厅科研项目(2011年度811210)
【分类号】：R737.14

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