PD-L1基因3’UTR单核苷酸多态性与膀胱尿路上皮癌关系的病例对照研究

发布时间：2018-04-13 16:00

本文选题：膀胱肿瘤 + 程序性死亡配体　；参考：《中国肿瘤生物治疗杂志》2017年07期

【摘要】：目的:探讨免疫关卡点分子程序性死亡配体1(programmed death ligand 1,PD-L1)基因3’端非翻译区(3’untranslated region,3’UTR)单核苷酸多态性(single nucleotide polymorphism,SNP)与膀胱尿路上皮癌(bladder urothelial carcinoma,BUC)发病风险及临床病理特征之间的关系。方法:采用病例-对照研究方法,PCR-LDR技术分别检测2013年6月至2015年12月在青岛大学附属医院泌尿外科住院手术治疗的213例BUC患者和251例同期健康体检者PD-L1基因3’UTR的rs4143815位点和rs2297136位点基因型分布频率,采用卡方检验和非条件多因素Logistic回归分析不同基因型与BUC发病风险以及临床病理特征之间的关系。结果:BUC组PD-L1基因3’UTR的rs4143815位点基因型分布频率与对照组相比存在明显差异,GG基因型个体发生BUC的风险是CC基因型的2.83倍(95%CI:1.82~4.64,P0.01),携带G突变基因(CG/GG基因型)个体BUC发病风险是CC型基因个体的1.53倍(95%CI:1.01~2.24,P0.01),同时BUC组rs4143815位点携带G突变基因频率与BUC病理分级和临床分期具有相关性(P0.05或P0.01);而在rs2297136位点,BUC组和对照组基因型分布频率无显著差异,CC、CT及TT基因型个体之间发生BUC的风险亦无显著差异(均P0.05)。结论:PD-L1基因3’UTR的rs4143815位点SNP与BUC的发病风险和恶性进展可能具有相关性。
[Abstract]:Objective: to investigate the relationship between single nucleotide polymorphisms (SNPs) of the 3'untranslated region (3untranslated region) of the 1(programmed death ligand 1 PD-L1) gene and the risk and clinicopathological features of bladder urothelial carcinomaBUC. objective: to investigate the relationship between the single nucleotide polymorphismSNPs and the clinicopathological features of the 3 'untranslated region of the 1(programmed death ligand 1 PD-L1 gene.Methods: PCR-LDR technique was used to detect PD-L1 gene 3' in 213 patients with BUC and 251 healthy controls from June 2013 to December 2015 in Urology Department, Qingdao University affiliated Hospital.The genotypes of rs4143815 locus and rs2297136 locus in UTR,Chi-square test and non-conditional multivariate Logistic regression analysis were used to analyze the relationship between different genotypes and the risk of BUC and clinicopathological features.Results there were significant differences in the frequency of genotype distribution of rs4143815 locus in PD-L1 gene 3'UTR between the two groups. The risk of developing BUC in individuals with GG genotype was 2.83 times higher than that in CC genotype. 95% CI: 1.82% 4.64% P0.01G and G mutation gene CGP / GG genotype were detected in individuals with BUC.The risk of the disease was 1.53 times as high as 95% of CC gene, and the frequency of G mutation gene at rs4143815 locus in BUC group was correlated with BUC pathological grade and clinical stage (P0.05 or P0.01), but there was no significant difference in genotype distribution between BUC group and control group in rs2297136 locus.There was no significant difference in the risk of BUC among individuals with CCT and TT genotypes (all P 0.05).Conclusion the rs4143815 SNP of the 3'UTR of the 1: PD-L1 gene may be associated with the risk and malignant progression of BUC.
【作者单位】：青岛大学附属医院儿科中心;青岛大学附属医院泌尿外科;
【基金】：国家自然科学基金资助项目(No.81101932) 青岛市应用基础研究计划资助项目(No.15-9-1-105-jch)~~
【分类号】：R737.14

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