磁共振T1rho及T1-mapping成像在大鼠肾缺血再灌注损伤的研究

发布时间：2018-04-13 16:30

本文选题：肾缺血再灌注损伤 + 磁共振成像　；参考：《南方医科大学》2017年硕士论文

【摘要】：第一部分磁共振Tlrho成像监测大鼠肾脏缺血再灌注损伤进程的初步研究研究目的研究3.0T磁共振Tlrho技术对大鼠肾IRI的发生、发展的监测价值,判断诊断大鼠不同肾损伤效能,并初步阐述该模型Tlrho值变化的影响因素。材料与方法制作同时夹闭双侧肾动脉45min制成大鼠肾IRI模型,将对照组及各模型组行磁共振Tlrho检查,扫描结束采取静脉血检测肾功能、处死取肾组织行病理检查,各组Tlrho值与病理结果统计学分析。结果各组血肌酐及尿素氮与对照组比较,仅1d组明显升高(P0.05),其余组稍升高,但无统计学差异且与肾损伤评分不存在相关性(P0.05)。对照组及模型组1h、1d、3d、7d、14d、28d 组的 Tlrho 值均数分别为:149.605±14.77ms、172.560±17.91ms、201.321±21.60ms、271.321±36.59ms、219.703±28.01ms、185.111±17.58ms、170.520±11.61ms,对照组与各模型组的Tlrho值的差异均具有统计学意义(P0.05)。模型组相邻两组比较,除了 14d组与28d组之外,其余组间的Tlrho值比较具有统计学差异。Tlrho值与肾损伤评分呈高度正相关,r=0.893,P0.01。利用ROC预测肾损伤由低-高评分组的AUC分别为0.934、0.957、0.946、0.980。结论磁共振Tlrho技术在监测大鼠肾IRI随时间的发展及判断肾损伤程度的应用中具有较高的价值。第二部分磁共振T1-mapping技术在大鼠肾IRI模型的初步应用以及与Tlrho成像的对比研究研究目的运用3.0T磁共振T1-mapping技术探讨皮髓质T1值在大鼠肾IRI的初步应用。对比研究T1-mapping与Tlrho技术在肾IRI模型的评价效能。材料与方法制作大鼠肾IRI模型后,将对照组及各模型组行磁共振T1-mapping检查,扫描结束后采取静脉血检测肾功能,处死后肾组织行病理检查,各组皮髓质T1值与病理结果进行统计学分析,各组T1值与Tlrho值对比研究。结果对照组及模型组 lh、1d、3d、7d、14d、28d 髓质 T1 值均数 1402.172±71.14ms、1398.475±95.54ms、1574.935±87.55ms、1732.881±110.06ms、1710.61±86.21ms、1550.904±127.49ms、1462.976±60.90ms;皮质 T1 值均值分别为1040.278±43.68ms、1094.6±123.00ms、1242.221±89.97ms、1417.038±115.81ms、1376.789±73.40ms、1148.500±90.19ms、1059.579±54.l0ms。对照组与各模型组的髓质、皮质T1值进行比较,均除了与lh及28d组无统计学差异外(P0.05),与其他组的差异均具有统计学意义(P0.05)。模型组相邻两组比较,皮髓质相邻两个时间点组间比较,均除了 3d组与7d组之间比较无统计学差异外,其余各组间的差异均有统计学意义。髓皮质T1值与肾损伤评分呈高度正相关,髓质相关系数r=0.769,P0.01,皮质r=0.787,P0.01。利用ROC预测肾损伤由低到高评分组,Tlrho值在各组的AUC均大于对应皮髓质T1值,Tlrho值、髓质 T1 值、皮质 T1 值得 AUC 各组分别为 0.934、0.957、0.946、0.980;0.792、0.834、0.925、0.920;0.836、0.887、0.919、0.915。结论 1、磁共振 T1-mapping成像在大鼠肾IRI模型中具有较高的应用价值。2、Tlrho对大鼠肾IRI模型的损伤程度诊断效能较T1-mapping好,两种技术联合运用,可以提高对肾IRI发生、发展的更全面的监测能力。
[Abstract]:The first part of magnetic resonance imaging for monitoring Tlrho renal ischemia reperfusion injury in rats to study the process of 3.0T magnetic resonance Tlrho technology on the kidney of IRI rats, the monitoring value of development, determine whether rat renal injury and discuss factors affecting the effectiveness of the model. The changes of Tlrho value of production materials and methods at the same time occlusion of bilateral renal artery 45min into rat renal IRI model, the control group and the model group underwent magnetic resonance Tlrho examination, the end of the scan take venous blood detection of renal function and renal tissue were sacrificed for histological examination, statistical analysis and pathological results were Tlrho value. Results the serum creatinine and urea nitrogen compared with the control group only, group 1D was significantly higher (P0.05), the other group was slightly increased, but the difference was not statistically significant and the score of renal injury and there is no correlation (P0.05). The control group and the model group, 1H, 1D, 3D, 7d, 14d, 28d group Tlrho The mean was 149.605 + 14.77ms, 172.560 + 17.91ms, 201.321 + 21.60ms, 271.321 + 36.59ms, 219.703 + 28.01ms, 185.111 + 17.58ms, 170.520 + 11.61ms, the control group and the model group differences in Tlrho values were statistically significant (P0.05). The model group adjacent to the two groups, except group 14d with the 28d group, the other groups, the Tlrho value was statistically difference in.Tlrho score was positively correlated with renal damage, r=0.893 damage, grouped by the low high rated AUC respectively with high application value 0.934,0.957,0.946,0.980. conclusion MRI Tlrho technique in renal IRI rats were monitored to determine the time with the development and severity of kidney damage the prediction of ROC by P0.01.. The second part renal magnetic resonance T1-mapping technology in the application of rat renal IRI model and Tlrho imaging to study the use of 3.0T magnetic resonance T1-mapping technology study Corticomedular T1 value preliminary application in rat renal IRI. A comparative study on T1-mapping and Tlrho technology in the evaluation of renal IRI model. The efficiency of rat renal IRI model making materials and methods, the control group and the model group underwent magnetic resonance T1-mapping examination, renal function tests take venous blood after scanning, after the execution of the kidney histologic examination, each medullary T1 values were analyzed statistically with pathological results, the T1 values of each group compared with the Tlrho value. The results of the control group and the model group LH, 1D, 3D, 7d, 14d, 28d, medulla T1 values are 1402.172 + 71.14ms, 1398.475 + 95.54ms, 1574.935 + 87.55ms, 1732.881 + 110.06ms. 1710.61 + 86.21ms, 1550.904 + 127.49ms, 1462.976 + 60.90ms; the mean cortical T1 values were 1040.278 + 43.68ms, 1094.6 + 123.00ms, 1242.221 + 89.97ms, 1417.038 + 115.81ms, 1376.789 + 73.40ms, 1148.500 + 90.19ms, 1059.579 + 54.l0ms. and the control group The model group of the medulla, cortex T1 value, are in addition to the LH and 28d group have no significant difference (P0.05), and the other group differences were statistically significant (P0.05). The model group adjacent to the two groups, corticomedular two adjacent time points were compared between the two groups, except between 3D and 7d group were no significant difference among other groups, the differences were statistically significant. Medullary cortical T1 values score was positively correlated with the renal medulla injury, correlation coefficient r=0.769, P0.01, r=0.787 P0.01. by ROC cortex, prediction of renal injury from low to high score, the Tlrho value corresponding to corticomedular T1 was greater than AUC in each group value, Tlrho value, T1 value is T1 medulla, cortex of AUC groups were 0.934,0.957,0.946,0.980; 0.792,0.834,0.925,0.920; 0.836,0.887,0.919,0.915. 1 conclusion, the application value of.2 T1-mapping magnetic resonance imaging with high IRI in kidney in a rat model of Tlrh. O is more effective than T1-mapping in diagnosing the degree of injury of rat kidney IRI model. Combined application of the two technologies can improve the monitoring ability of the occurrence and development of kidney IRI.

【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R445.2;R692

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