尿路上皮癌诊断中DNA倍体分析和尿脱落细胞学检查的临床价值

发布时间：2018-04-14 17:25

本文选题：膀胱肿瘤 + 尿路上皮癌　；参考：《临床与实验病理学杂志》2015年06期

【摘要】：目的探讨DNA倍体分析和尿脱落细胞学检查对尿路上皮癌诊断的临床价值,寻找早期诊断尿路上皮癌的理想方法。方法随机收集121例尿路上皮癌患者的尿液标本作为实验组,另取95例良性血尿非肿瘤患者的尿液标本作为对照组,分别进行尿脱落细胞学检查和DNA倍体分析。结果尿脱落细胞学检查的敏感性高于DNA倍体分析,但特异性低于DNA倍体分析。尿脱落细胞学检查敏感性为85.10%,特异性为76.80%;DNA倍体分析敏感性为81.80%,特异性为81.10%。121例中,膀胱癌103例,上尿路上皮癌18例。对于不同分型的尿路上皮癌,两种方法在高级别肿瘤中均有较好的敏感性,尤其是DNA倍体分析在浸润性膀胱癌和上尿路上皮癌的诊断中具有极好的敏感性,均94%。结论 DNA倍体分析结合尿脱落细胞学检查,可大大提高尿路上皮癌检测的特异性,以及增加对浸润性膀胱癌及上尿路上皮癌诊断的灵敏度,是对尿路上皮癌检测方法的有利补充。
[Abstract]:Objective to explore the clinical value of DNA ploidy analysis and urine exfoliative cytology in the diagnosis of urothelial carcinoma and to find an ideal method for early diagnosis of urothelial carcinoma.Methods urine samples from 121 patients with urothelial carcinoma were randomly collected as experimental group and 95 patients with benign hematuria as control group. Urine exfoliative cytology and DNA ploidy analysis were performed respectively.Results the sensitivity of urine exfoliative cytology was higher than that of DNA ploidy analysis, but the specificity was lower than that of DNA ploidy analysis.The sensitivity of urine exfoliative cytology was 85.10, the specificity was 76.80 and the sensitivity of DNA ploidy analysis was 81.80, the specificity was 81.10.121, bladder cancer 103 cases, upper urinary epithelial carcinoma 18 cases.For different types of urothelial carcinoma, both methods have a good sensitivity in high grade tumors, especially DNA ploidy analysis has excellent sensitivity in the diagnosis of invasive bladder cancer and upper urinary epithelial carcinoma.Conclusion DNA ploidy analysis combined with urine exfoliative cytology can greatly improve the specificity of the detection of urothelial carcinoma and increase the sensitivity in the diagnosis of invasive bladder cancer and upper urinary epithelial carcinoma.It is a favorable supplement to the detection method of urothelial carcinoma.
【作者单位】：复旦大学遗传工程国家重点实验室;
【分类号】：R737.1

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