人β-防御素-2基因预防感染性结石的实验研究

发布时间：2018-04-17 13:38

本文选题：人β-防御素-2 + 基因预防　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的：通过对膀胱感染性结石大鼠膀胱灌注脂质体包裹的人β-防御素-2重组真核表达载体（pCAGG-hBD2），观察人β-防御素-2（hBD2）基因对感染性结石的预防作用。方法：健康成年雄性SD大鼠40只，随机分为实验组与对照组，实验组膀胱灌注给予pCAGG-hBD2250μl，对照组经同法给予等量空载体（pCAGG），2天后两组均随机处死3只大鼠，利用小动物成像仪观察重组hBD2在SD大鼠膀胱粘膜的表达，转染成功后两组均膀胱内置入含菌（奇异变形杆菌）聚乙烯管异物。分别于24、48、72小时收集尿液，，进行尿液菌落计数、白细胞计数，30天处死全部大鼠获取膀胱组织，观察结石的形成情况及观察膀胱组织病理学变化。结果:1、实验组大鼠置入含菌（奇异变形杆菌）聚乙烯管异物24、48、72小时后尿液中奇异变形杆菌菌落总数、尿白细胞计数均显著低于对照组，组间差异均有统计学意义（P0.001）；实验组和对照组不同时间大鼠尿液中奇异变形杆菌菌落总数及尿白细胞计数差异有统计学意义。2、对照组大鼠的尿路组织主要表现为肾输尿管膀胱的炎症改变，膀胱内有感染性结石的形成；实验组大鼠的尿路组织病理学无明显变化，膀胱内无结石生成。结论：1、重组hBD2基因对奇异变形杆菌致泌尿系感染性结石有预防作用。 2、重组hBD2基因可增强尿路粘膜的抗感染能力。
[Abstract]:Objective : To observe the effect of human 尾 - defensu - 2 ( hBD2 ) gene on infectious calculus by the recombinant eukaryotic expression vector pCAGG - hBD2 .

Methods : Forty - four healthy male SD rats were randomly divided into experimental group and control group . The experimental group was given pCAGG - hBD222渭l . The control group was given equal amount of empty vector ( pCAGG ) . Two days later , three rats were sacrificed randomly . The urine was collected in 24 , 48 and 72 hours after transfection . All the rats were sacrificed at 24 , 48 and 72 hours . All the rats were sacrificed to obtain bladder tissue , the formation of calculus was observed and the pathological changes of bladder tissues were observed .

Results : 1 . In the experimental group , 24 , 48 and 72 hours later , the total number of bacteria colony and urine white blood cell count in urine were significantly lower than those in the control group ( P 0.001 ) .
In the experimental group and control group , there was a significant difference in the total number of bacteria colony and urine white blood cell count in urine of rats with different time . 2 . The urinary tract structure of the control group was mainly the inflammatory change of the urinary bladder and the formation of infectious calculus in the bladder ;
There was no obvious change in the pathology of urinary tract in the experimental group , and no stone formation in the bladder .

Conclusion : 1 . The recombinant hBD2 gene has a preventive effect on the urinary tract infection of the urinary system .

2 . The recombinant hBD2 gene can enhance the anti - infective ability of urinary tract mucosa .

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R691.3

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