超抗原葡萄球菌肠毒素B诱导IgA肾病小鼠模型的建立

发布时间：2018-04-22 16:33

本文选题：IgA肾病 + 动物模型　；参考：《中国比较医学杂志》2017年12期

【摘要】：目的建立Ig A肾病小鼠模型,并观察模型的生化及病理指标特点。方法 12只BALB/c小鼠随机分为正常组(6只)和模型组(6只),模型组单次尾静脉注射葡萄球菌肠毒素B(SEB)0.8 mg/kg,第二周开始,连续注射三周,第四周结束后检测小鼠24 h尿蛋白定量,尿微量白蛋白,肾功能BUN、Scr、UA;蛋白指标TP、ALB;肝功能ALT、AST、ALP;血脂TC、TG、LDL的情况,肾脏免疫荧光Ig A的沉积,肾脏病理HE、PAS、PASM、Masson染色以及透射电镜的观察肾脏超微结构,以及肝脏与小肠HE染色、免疫荧光Ig A的沉积变化。结果与正常组比较,模型组小鼠24 h尿蛋白定量与尿微量白蛋白均升高(P0.01);模型组小鼠肾功能指标CREA,UA均高于正常组(P0.05),BUN差异无显著性;模型组小鼠蛋白指标TP、ALB差异无显著性;模型组小鼠肝功能指标AST水平高于正常组(P0.05),ALT、ALP差异无显著性;模型组小鼠血脂TG水平低于正常组(P0.05),LDL水平高于正常组(P0.01),TC差异无显著性。肾脏免疫荧光检查可见模型组小鼠肾小球系膜区呈颗粒状、团块状的Ig A沉积;模型组小鼠肾脏病理轻中度损伤,系膜区免疫复合物增多;模型组小鼠肝脏HE染色可见少量炎性细胞浸润伴有部分肝细胞坏死,小肠绒毛缺损,肠绒毛变短变细、间距明显增宽,有部分分上皮脱落,中央央乳糜管扩张显著,淋巴细胞增多,明显可见炎性细胞浸润。结论使用超抗原SEB尾静脉注射小鼠可以成功复制Ig A肾病动物模型。
[Abstract]:Objective to establish a mouse model of IgA nephropathy and observe the biochemical and pathological characteristics of the model. Methods Twelve BALB/c mice were randomly divided into normal group (n = 6) and model group (n = 6). Staphylococcal enterotoxin B(SEB)0.8 mg / kg was injected into the tail vein of model group. Urinary Microalbumin, Renal function Bun ScrUAA; protein Index TPTASALB; liver function alt ASTGN ALP; Lipid TCU TGG LDL; deposition of Renal Immunofluorescence IgA; Renal Pathology, PASPASMMA Masson staining and Transmission Electron microscope observation of Renal Ultrastructure, liver and small intestine HE staining, Changes of immunoreactive deposition of IgA. Results compared with the normal group, the urine protein quantity and urinary microalbumin in the model group increased significantly (P 0.01), the renal function index of the model group was higher than that of the normal group (P 0.05 bun), the protein index of the model group was not significantly different from that of the control group, and there was no significant difference between the model group and the control group. There was no significant difference in AST level between the model group and the normal group, but there was no significant difference between the model group and the normal group. Renal immunofluorescence showed that glomerular Mesangial area was granular and massive IgA was deposited in the model group, and the renal pathology of model group was mild to moderate injury, and the immune complex of Mesangial region was increased in the model group. In the model group, a small amount of inflammatory cells infiltrated with hepatocyte necrosis, small intestinal villi defect, the intestinal villi became shorter and thinner, the spacing was obviously widened, some of the epithelium fell off, and the central central chylotube dilated significantly. Lymphocyte increase, inflammatory cell infiltration can be seen obviously. Conclusion the model of IgA nephropathy can be successfully established by injecting superantigen SEB into tail vein of mice.
【作者单位】：上海中医药大学附属曙光医院肾病科上海中医药大学中医肾病研究所肝肾疾病病证教育部重点实验室上海市中医临床重点实验室;
【基金】：上海市中医药事业发展三年行动计划项目(ZYSNXD-CC-YJXYY);上海市中医药事业发展三年行动计划项目(ZYSNXD-CCMZY044)
【分类号】：R-332;R692.31

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