强精片对雷公藤所致不育症大鼠精液质量的影响及机制

发布时间：2018-04-28 10:14

本文选题：不育症 + 精液质量　；参考：《山东医药》2017年20期

【摘要】：目的探讨益肾活血利湿中药强精片对雷公藤所致不育症大鼠精液质量的影响及其机制。方法实验动物为50只雄性SD大鼠,10只作为空白组,另40只以雷公藤灌胃制作不育症模型并随机等分为模型组及强精片低、中、高剂量组,四组均予雷公藤20 mg/(kg·d)灌胃;强精片低、中、高剂量组同时分别予强精片58、105、233 mg/(kg·d)灌胃;空白组予等量生理盐水灌胃;均1次/d。灌胃后4周检测各组精液质量、血清性激素水平及睾丸组织Cx43蛋白表达。结果各强精片组精液质量均好于模型组,血清睾酮水平及睾丸组织Cx43蛋白表达均高于模型组,且呈现出一定的量效关系(P0.05或0.01)。结论益肾活血利湿中药强精片可改善不育症大鼠的精液质量,其作用机制可能为调节血清睾酮水平及睾丸组织Cx43蛋白表达。
[Abstract]:Objective to investigate the effect of Qiangjing tablet on semen quality of rats with infertility induced by Tripterygium wilfordii. Methods Fifty male Sprague-Dawley rats were used as blank group, and 40 male SD rats were treated with tripterygium wilfordii gavage for 20 mg/(kg / d, and 40 male SD rats were divided into two groups randomly: model group, low dose group, middle and high dose group, and four groups were treated with tripterygium wilfordii for 20 mg/(kg d. The middle and high dose groups were treated with Qiangjing tablet for 58105233 mg/(kg d, while the blank group were given the same amount of normal saline. Semen quality, serum sex hormone level and Cx43 protein expression in testis were measured 4 weeks after gastric perfusion. Results the semen quality of each group was better than that of the model group. The level of serum testosterone and the expression of Cx43 protein in testis were higher than those in the model group, and there was a dose-effect relationship between them (P0.05 or 0.01). Conclusion Qiangjing tablet can improve the semen quality of infertility rats by regulating serum testosterone level and Cx43 protein expression in testis.
【作者单位】：郑州人民医院;成都中医药大学临床医学院;成都中医药大学附属医院;成都中医药大学第二附属医院;
【基金】：国家自然科学基金资助项目(81302983)
【分类号】：R698.2

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