初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系

发布时间：2018-05-02 16:03

本文选题：膀胱ICC + 增殖　；参考：《第三军医大学学报》2017年02期

【摘要】：目的探讨初生至成年时期大鼠膀胱Cajal间质细胞(interstitial cells of Cajal,ICC)增殖变化情况及其与相应时间点膀胱兴奋性的关系。方法 40只刚出生的大鼠分为2组,每组20只。第1组从出生后第1、15、30、45、60天对其膀胱进行c-kit和Ki67染色,观察ICC细胞的增殖情况;第2组在上述同一时间对其膀胱进行c-kit和SM-actin染色,共聚焦显微镜下观察ICC的分布密度,并测定同期膀胱肌条的兴奋性。结果膀胱ICC细胞的增殖速度和分布密度从第1天开始逐步增加,到第30天后增殖速度明显下降,分布密度也随之趋于稳定。大鼠逼尿肌肌条兴奋性从出生后第1天逐渐下降,第45~60天趋于稳定。结论大鼠膀胱ICC细胞在出生后迅速增殖,到第45天分布密度趋于稳定。膀胱兴奋性随着ICC分布密度的增加逐渐降低,并趋于稳定。
[Abstract]:Objective to investigate the proliferation of interstitial cells of Cajal in rat bladder from birth to adulthood and its relationship with bladder excitability at the corresponding time point. Methods Forty newborn rats were divided into 2 groups, 20 rats in each group. The proliferation of ICC cells was observed by c-kit and Ki67 staining in the bladder of the first group, and the distribution of ICC was observed by confocal microscope under confocal microscope, c-kit and SM-actin staining were performed on the bladder at the same time. The excitability of bladder muscle strips at the same time was measured. Results the proliferation rate and distribution density of bladder ICC cells increased gradually from the first day to the 30th day, and the distribution density tended to be stable. The excitability of detrusor muscle strips decreased gradually from the first day after birth and stabilized on the 45th day. Conclusion Rat bladder ICC cells proliferate rapidly after birth, and the density of bladder ICC cells tends to be stable at day 45. The excitability of bladder decreased gradually with the increase of ICC density and tended to be stable.
【作者单位】：第三军医大学西南医院全军泌尿外科中心;
【基金】：国家自然科学基金面上项目(81100495)~~
【分类号】：R694

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