MicroRNA-200c、ZEB1及ZEB2在膀胱癌中的表达及意义

发布时间：2018-05-04 12:03

本文选题：膀胱癌 + MicroRNA-200c　；参考：《天津医科大学》2014年硕士论文

【摘要】：目的：观察microRNA-200c及转录因子ZEB1. ZEB2(SIP1)在膀胱癌组织中的表达情况,并分析其与肿瘤数目、肿瘤大小、病理分级、临床分期等临床病理学参数之间的关系。探讨microRNA-200c与ZEB1和ZEB2在膀胱癌组织中表达的相关性,从而为进一步研究microRNA-200c在膀胱癌EMT过程中的作用及其可能的作用机制提供依据,并阐明其在膀胱癌患者中的临床预后意义。方法：对58例膀胱癌组织(实验组)及58例正常膀胱粘膜组织(对照组)采用实时定量PCR方法检测microRNA-200c的相对表达量,采用免疫组织化学方法(超敏型S-P法)检测ZEB1、ZEB2的表达情况,分别比较三者在实验组和对照组中的表达差异,并分析三者在膀胱癌组织中的表达与肿瘤数目、肿瘤大小、病理分级、临床分期等临床病理学参数之间的关系,进一步分析其表达之间的关联性。结果： 1. MicroRNA-200c及ZEB1、ZEB2在膀胱癌中的表达均高于其在正常膀胱粘膜中的表达(P0.05)。 2.膀胱癌中microRNA-200c的表达差异与肿瘤数目及肿瘤大小无关(P0.05),而与肿瘤病理分级及临床分期有关,microRNA-200c的表达强度在非肌层浸润性膀胱癌组高于肌层浸润性膀胱癌组(P0.05),在低分级膀胱癌组高于高分级膀胱癌组(P0.05)。 3.膀胱癌中ZEB1及ZEB2的表达差异与肿瘤数目及肿瘤大小无关(P0.05),而与肿瘤病理分级及临床分期有关,ZEB1及ZEB2在肌层浸润性膀胱癌中的表达阳性率高于其在非肌层浸润性膀胱癌中的表达阳性率(P0.05),在高分级膀胱癌中的表达阳性率高于其在低分级膀胱癌中的表达阳性率(P0.05)。 4.在膀胱癌组织中,microRNA-200c的表达水平与ZEB1的表达之间有明显相关性,但和ZEB2的表达之间无明显相关性,microRNA-200c的表达量在ZEB1阴性组明显高于ZEB1阳性组(P0.05),但在ZEB2阴性组和ZEB2阳性组之间无明显差异(P0.05)。结论： 1. MicroRNA-200c在膀胱癌中的表达强度随肿瘤病理分级和临床分期的增加而降低,ZEB1和ZEB2在膀胱癌中的表达水平随肿瘤病理分级和临床分期的增加而增高,提示它们可能参与了膀胱癌的发生、发展过程,并在其侵袭和转移过程中发挥作用。通过检测膀胱癌中microRNA-200c及ZEB1、ZEB2的表达有助于膀胱癌的诊断和治疗,并可能成为判断膀胱癌预后的重要指标。 2. MicroRNA-200c在膀胱癌中的表达与ZEB1的表达之间存在明显相关性,在microRNA-200c表达上调时,会出现ZEB1的低表达,提示ZEB1的表达可能受到microRNA-200c的调节,ZEB1可能是microRNA-200c的靶基因,它们共同参与了膀胱癌的EMT过程。 3.本研究为抑制膀胱癌的侵袭和转移提供了新的研究方向,ZEB1、ZEB2有望成为治疗膀胱癌新的干预靶点,microRNA-200c可能成为膀胱癌新的肿瘤标记物。
[Abstract]:Objective: MicroRNA-200c and transcription factor ZEB1 were observed. The expression of ZEB2 and SIP1 in bladder cancer and its relationship with tumor number, tumor size, pathological grade, clinical stage and other clinicopathological parameters were analyzed. To investigate the correlation between microRNA-200c, ZEB1 and ZEB2 expression in bladder cancer tissues, so as to provide a basis for further study on the role of microRNA-200c in the process of bladder cancer EMT and its possible mechanism, and to elucidate the clinical prognostic significance of microRNA-200c in bladder cancer patients. Methods: The relative expression of microRNA-200c in 58 cases of bladder cancer (experimental group) and 58 cases of normal bladder mucosa (control group) was detected by real-time quantitative PCR, and the expression of ZEB1 and ZEB2 was detected by immunohistochemistry (hypersensitive S-P method). To compare the difference of expression between the three groups in the experimental group and the control group, and to analyze the relationship between the expression of the three proteins and the clinicopathological parameters, such as tumor number, tumor size, pathological grade, clinical stage, and so on. Further analysis of the relationship between its expression. Results: 1. The expression of MicroRNA-200c and ZEB2 in bladder cancer was higher than that in normal bladder mucosa. 2. The difference of microRNA-200c expression in bladder cancer was not related to the number and size of tumor, but the expression of microRNA-200c was related to the pathological grade and clinical stage of the tumor. The expression of microRNA-200c in non-muscular invasive bladder cancer group was higher than that in myometrial invasive bladder cancer group, and in the low score, the expression level of microRNA-200c was higher in non-myometrial invasive bladder cancer group than in myometrial invasive bladder cancer group. The grade grade bladder cancer group was higher than the high grade bladder cancer group (P 0.05). 3. The difference of ZEB1 and ZEB2 expression in bladder cancer is not related to the number and size of tumor, but the positive rate of ZEB1 and ZEB2 in myometrial invasive bladder cancer is higher than that in non-myometrial invasive bladder cancer. The positive rate of cysteine carcinoma was higher in high grade bladder cancer than in low grade bladder cancer. 4. There was a significant correlation between the expression of microRNA-200c and the expression of ZEB1 in bladder cancer. The expression of microRNA-200c in ZEB1 negative group was significantly higher than that in ZEB1 positive group, but there was no significant difference between ZEB2 negative group and ZEB2 positive group. Conclusion: 1. The expression of MicroRNA-200c in bladder cancer decreased with the increase of tumor pathological grade and clinical stage, and the expression of ZEB1 and ZEB2 in bladder cancer increased with the increase of tumor pathological grade and clinical stage, suggesting that they may be involved in the occurrence of bladder cancer. Development process and play a role in its invasion and metastasis. The expression of microRNA-200c and ZEB1 / ZEB2 in bladder cancer is helpful to the diagnosis and treatment of bladder cancer and may be an important index to judge the prognosis of bladder cancer. 2. There was a significant correlation between the expression of MicroRNA-200c and the expression of ZEB1 in bladder cancer. When the expression of microRNA-200c was up-regulated, the low expression of ZEB1 appeared, suggesting that the expression of ZEB1 might be regulated by microRNA-200c. ZEB1 might be the target gene of microRNA-200c. They are involved in the EMT process of bladder cancer. 3. This study provides a new research direction for inhibiting invasion and metastasis of bladder cancer. ZEB1 / ZEB2 may be a new intervention target for bladder cancer. MicroRNA-200c may be a new tumor marker for bladder cancer.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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